Biotechnological applications of the red alga Furcellaria lumbricalis and its cultivation potential in the Baltic Sea

Kersen, Priit; Paalme, Tiina; Pajusalu, Liina; Martin, Georg

doi:10.1515/bot-2016-0062

Cited by 16 publications

(10 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…R-phycoerythrin (R-PE) is one of the major phycobiliproteins in P. yezoensis. Its purity is generally calculated by the absorbance ratio (A 565 /A 280 ), and the market price is around 200 USD/mg (Sigma-Aldrich, product number: 52412-1MG-F) [7,8]. Therefore, R-PE is regarded as a bioactive molecule with high value.…”

Section: R-phycoerythrin (R-pementioning

confidence: 99%

Efficient Purification of R-phycoerythrin from Marine Algae (Porphyra yezoensis) Based on a Deep Eutectic Solvents Aqueous Two-Phase System

Wang

Hou

2020

Marine Drugs

View full text Add to dashboard Cite

R-phycoerythrin (R-PE), a marine bioactive protein, is abundant in Porphyra yezoensis with high protein content. In this study, R-PE was purified using a deep eutectic solvents aqueous two-phase system (DES-ATPS), combined with ammonium sulphate precipitation, and characterized by certain techniques. Firstly, choline chloride-urea (ChCl-U) was selected as the suitable DES to form ATPS for R-PE extraction. Then, single-factor experiments were conducted: the purity (A565/A280) of R-PE was 3.825, and the yield was 69.99% (w/w) under optimal conditions (adding 0.040 mg R-PE to ChCl-U (0.35 g)/K2HPO4 (0.8 g/mL, 0.5 mL) and extracting for 20 min). The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) results revealed that the purified R-PE contained three main bands. One band was presented after purification in native-PAGE. The UV-vis spectra showed characteristic absorption peaks at 495, 540, and 565 nm. R-PE displayed an emission wavelength at 570 nm when excited at 495 nm. All spectra results illustrated that the structure of R-PE remained unchanged throughout the process, proving the effectiveness of this method. Transmission electron microscope (TEM) showed that aggregation and surrounding phenomena were the driving forces for R-PE extraction. This study could provide a green and simple purification method of R-PE in drug development.

show abstract

Section: R-phycoerythrin (R-pementioning

confidence: 99%

Efficient Purification of R-phycoerythrin from Marine Algae (Porphyra yezoensis) Based on a Deep Eutectic Solvents Aqueous Two-Phase System

Wang

Hou

2020

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…seasonality, water transparency, depth and density of the algal community (Martin et al 2006a,b, Kotta et al 2008, Paalme et al 2011. The commercial utilization of the loose-lying F. lumbricalis and Coccotylus truncatus community in Kassari Bay was started in 1966 by the local company ESTAGAR (ESTAGAR 2019) and until now it has been mostly based on the extraction of furcellaran, that is widely used as a stabilizing, thickening and gelling agent in the food, pharmaceutical, cosmetics and agriculture industries (Tuvikene et al 2006, Tuvikene and Robal 2015a, Kersen et al 2017. Furcellarans of F. lumbricalis from the Baltic Sea are characterized by unique chemical composition and properties, as they are a hybrid of κ and β carrageenan (Tuvikene et al 2006, Tuvikene andRobal 2015a).…”

Section: Harvest Of Furcellaria Lumbricalismentioning

confidence: 99%

“…At present, there is an increasing interest in a new potential biotechnological application of unattached F. lumbricalis biomass as a raw material for extraction of the red pigment R-phycoerythrin Robal 2015b, Kersen et al 2017). Due to its different bioactive properties, R-phycoerythrin can be used not only in the food industry as a natural food colorant, but also in medicine and cosmetics (Kersen et al 2017).…”

Section: Harvest Of Furcellaria Lumbricalismentioning

confidence: 99%

Seaweed resources of the Baltic Sea, Kattegat and German and Danish North Sea coasts

2019

Self Cite

View full text Add to dashboard Cite

Due to low salinity and lack of hard substrata, the Baltic Sea and Kattegat area and German and Danish North Sea coasts are characterized by a relatively low diversity of seaweeds. At the same time the areas are severely eutrophicated, which has caused extensive shifts in macroalgal communities toward opportunistic species. Unattached seaweed communities dominated by Furcellaria lumbricalis, which have been a resource for hydrocolloid production since the 1940s, have been severely reduced due to eutrophication and unsustainable harvesting and are nowadays only exploited commercially in Estonia. On the other hand, the biomass of opportunistic seaweeds of various red, green and brown algal genera has increased. They cause ecological problems, are a nuisance on many tourist beaches and constitute at the same time a potential bioresource that is so far only exploited to a limited extent for production of energy and fertilizer. Commercial seaweed cultivation is largely focused on Saccharina latissima and still very limited, but is currently being expanded as a compensation measure for sea-based fish aquaculture. Also land-based seaweed cultivation is primarily employed for recycling of nutrients in tank animal aquaculture, but in most cases so far only on an experimental scale.

show abstract

“…Commercial PE from microalgae has been produced by Spirulina , Rhodella , and Porphyridium [10,11,12]. The market price of this purified pigment is around $200 USD mg −1 (Sigma-Aldrich, product number: 52412-1MG-F), this being why PE is regarded as a molecule of high value [13]. Porphyridium purpureum has the ability to grow in different culture systems and locations [14], coupled with the capacity to produce and accumulate a large amount of PE, which production can be enhanced when the microalgae are cultured under stress conditions [11].…”

Section: Introductionmentioning

confidence: 99%

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

et al. 2019

View full text Add to dashboard Cite

Several factors have the potential to influence microalgae growth. In the present study, nitrogen concentration and light intensity were evaluated in order to obtain high biomass production and high phycoerythrin accumulation from Porphyridium purpureum. The range of nitrogen concentrations evaluated in the culture medium was 0.075–0.450 g L−1 and light intensities ranged between 30 and 100 μmol m−2 s−1. Surprisingly, low nitrogen concentration and high light intensity resulted in high biomass yield and phycoerythrin accumulation. Thus, the best biomass productivity (0.386 g L−1 d−1) and biomass yield (5.403 g L−1) were achieved with NaNO3 at 0.075 g L−1 and 100 μmol m−2 s−1. In addition, phycoerythrin production was improved to obtain a concentration of 14.66 mg L−1 (2.71 mg g−1 of phycoerythrin over dry weight). The results of the present study indicate that it is possible to significantly improve biomass and pigment production in Porphyridium purpureum by limiting nitrogen concentration and light intensity.

show abstract

Biotechnological applications of the red alga Furcellaria lumbricalis and its cultivation potential in the Baltic Sea

Abstract: There is a growing need for diversification of seaweed aquaculture practices in Europe. In the Baltic Sea, very few seaweed species are utilised commercially, and only the kelp

Cited by 16 publications

References 64 publications

Efficient Purification of R-phycoerythrin from Marine Algae (Porphyra yezoensis) Based on a Deep Eutectic Solvents Aqueous Two-Phase System

Efficient Purification of R-phycoerythrin from Marine Algae (Porphyra yezoensis) Based on a Deep Eutectic Solvents Aqueous Two-Phase System

Seaweed resources of the Baltic Sea, Kattegat and German and Danish North Sea coasts

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Contact Info

Product

Resources

About