Biotechnological Applications of Products Released by Marine Microorganisms for Cold Adaptation Strategies: Polyunsaturated Fatty Acids, Antioxidants, and Antifreeze Proteins

Lauritano, Chiara; Coppola, Daniela

doi:10.3390/jmse11071399

Cited by 3 publications

(2 citation statements)

References 149 publications

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“…Arachidonic acid methyl ester (C20:4n-6) was the most abundant PUFAs, followed by α-linolenic acids (C18:3) and linoleic acids (C18:2) methyl esters. PUFAs synthesis at cold temperature represents a well-known strategy for the maintenance of membrane fluidity in microalgae [48][49][50]. Our results are consistent with those reported for the Antarctic diatom Navicula UMACC 231, which showed around 30% of PUFAs when cultivated at 4 • C [50].…”

Section: Discussionsupporting

confidence: 91%

Bioprospection of the Antarctic Diatoms Craspedostauros ineffabilis IMA082A and Craspedostauros zucchelli IMA088A

Trentin,

Moschin,

Custódio

et al. 2024

Marine Drugs

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In extreme environments such as Antarctica, a diverse range of organisms, including diatoms, serve as essential reservoirs of distinctive bioactive compounds with significant implications in pharmaceutical, cosmeceutical, nutraceutical, and biotechnological fields. This is the case of the new species Craspedostauros ineffabilis IMA082A and Craspedostauros zucchellii IMA088A Trentin, Moschin, Lopes, Custódio and Moro (Bacillariophyta) that are here explored for the first time for possible biotechnological applications. For this purpose, a bioprospection approach was applied by preparing organic extracts (acetone and methanol) from freeze-dried biomass followed by the evaluation of their in vitro antioxidant properties and inhibitory activities on enzymes related with Alzheimer’s disease (acetylcholinesterase: AChE, butyrylcholinesterase: BChE), Type 2 diabetes mellitus (T2DM, α–glucosidase, α–amylase), obesity (lipase) and hyperpigmentation (tyrosinase). Extracts were then profiled by ultra-high-performance liquid chromatography–mass spectrometry (UPLC–HR–MS/MS), while the fatty acid methyl ester (FAME) profiles were established by gas chromatography–mass spectrometry (GC–MS). Our results highlighted strong copper chelating activity of the acetone extract from C. ineffabilis and moderate to high inhibitory activities on AChE, BChE, α–amylase and lipase for extracts from both species. The results of the chemical analysis indicated polyunsaturated fatty acids (PUFA) and their derivatives as the possible compounds responsible for the observed activities. The FAME profile showed saturated fatty acids (SFA) as the main group and methyl palmitoleate (C16:1) as the predominant FAME in both species. Overall, our results suggest both Antarctic strains as potential sources of interesting molecules with industrial applications. Further studies aiming to investigate unidentified metabolites and to maximize growth yield and natural compound production are required.

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Section: Discussionsupporting

confidence: 91%

Bioprospection of the Antarctic Diatoms Craspedostauros ineffabilis IMA082A and Craspedostauros zucchelli IMA088A

Trentin,

Moschin,

Custódio

et al. 2024

Marine Drugs

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“…Moreover, in this Special Issue, Lauritano and Coppola [6] reviewed bacterial and microalgal compounds of great interest for the market, which are produced as strategies implemented by microorganisms during the cold-adaptation process. In particular, they focused on polyunsaturated fatty acids, antioxidants, and antifreeze proteins, for which the compound annual growth rate is expected to increase by 5.35-36.3% in the near future.…”

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confidence: 99%

Biodiversity, Adaptation Strategies, and Opportunities in Extreme Marine Environments

Lauritano,

Coppola

2023

JMSE

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Salinity Stress Acclimation Strategies in Chlamydomonas sp. Revealed by Physiological, Morphological and Transcriptomic Approaches

Lauritano,

Bazzani,

Montuori

et al. 2024

Marine Drugs

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Climate changes may include variations in salinity concentrations at sea by changing ocean dynamics. These variations may be especially challenging for marine photosynthetic organisms, affecting their growth and distribution. Chlamydomonas spp. are ubiquitous and are often found in extreme salinity conditions. For this reason, they are considered good model species to study salinity adaptation strategies. In the current study, we used an integrated approach to study the Chlamydomonas sp. CCMP225 response to salinities of 20‰ and 70‰, by combining physiological, morphological, and transcriptomic analyses, and comparing differentially expressed genes in the exponential and stationary growth phases under the two salinity conditions. The results showed that the strain is able to grow under all tested salinity conditions and maintains a surprisingly high photosynthetic efficiency even under high salinities. However, at the highest salinity condition, the cells lose their flagella. The transcriptomic analysis highlighted the up- or down-regulation of specific gene categories, helping to identify key genes responding to salinity stress. Overall, the findings may be of interest to the marine biology, ecology, and biotechnology communities, to better understand species adaptation mechanisms under possible global change scenarios and the potential activation of enzymes involved in the synthesis of bioactive molecules.

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Biotechnological Applications of Products Released by Marine Microorganisms for Cold Adaptation Strategies: Polyunsaturated Fatty Acids, Antioxidants, and Antifreeze Proteins

Cited by 3 publications

References 149 publications

Bioprospection of the Antarctic Diatoms Craspedostauros ineffabilis IMA082A and Craspedostauros zucchelli IMA088A

Bioprospection of the Antarctic Diatoms Craspedostauros ineffabilis IMA082A and Craspedostauros zucchelli IMA088A

Biodiversity, Adaptation Strategies, and Opportunities in Extreme Marine Environments

Salinity Stress Acclimation Strategies in Chlamydomonas sp. Revealed by Physiological, Morphological and Transcriptomic Approaches

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