Biotechnological applications of mammalian odorant-binding proteins

Gonçalves, Filipa; Ribeiro, Artur; Silva, Carla

doi:10.1080/07388551.2020.1853672

Cited by 13 publications

(4 citation statements)

References 96 publications

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“…Because vertebrate OBPs are remarkably stable, relatively easy to express, and have the desired binding properties that can be modified through site-directed mutagenesis and molecular modeling [30,31], they have great potential for biotechnological applications [32,33]. OBPs can be used for the development of sensing elements in biosensors for odor monitoring, clinical analysis, or food sensory evaluation [34][35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Ligand Binding Properties of Odorant-Binding Protein OBP5 from Mus musculus

Moitrier

Belloir

Lalis

et al. 2022

Biology

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Odorant-binding proteins (OBPs) are abundant soluble proteins secreted in the nasal mucus of a variety of species that are believed to be involved in the transport of odorants toward olfactory receptors. In this study, we report the functional characterization of mouse OBP5 (mOBP5). mOBP5 was recombinantly expressed as a hexahistidine-tagged protein in bacteria and purified using metal affinity chromatography. The oligomeric state and secondary structure composition of mOBP5 were investigated using gel filtration and circular dichroism spectroscopy. Fluorescent experiments revealed that mOBP5 interacts with the fluorescent probe N-phenyl naphthylamine (NPN) with micromolar affinity. Competitive binding experiments with 40 odorants indicated that mOBP5 binds a restricted number of odorants with good affinity. Isothermal titration calorimetry (ITC) confirmed that mOBP5 binds these compounds with association constants in the low micromolar range. Finally, protein homology modeling and molecular docking analysis indicated the amino acid residues of mOBP5 that determine its binding properties.

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Section: Introductionmentioning

confidence: 99%

Ligand Binding Properties of Odorant-Binding Protein OBP5 from Mus musculus

Moitrier

Belloir

Lalis

et al. 2022

Biology

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“…These sensory data are then transmitted to various brain regions for further interpretation by the complex neural circuitry of the olfactory bulb (Sharma et al., 2019). Initially, aroma compounds bind reversibly to odorant‐binding proteins (OBPs), which transport hydrophobic aroma compounds across the nasal mucus to bind with ORs within the mucus, triggering a sequence of intracellular events culminating in an electrical signal to the brain's olfactory bulb (Goncalves et al., 2021). Notably, in contrast to other mammals, only two OBP genes, OBP2A and OBP2B, are expressed in humans and their expressions in the human OE do not exhibit any enhancement (Olender et al., 2016).…”

Section: The Brain's Role In Aroma Perceptionmentioning

confidence: 99%

The coming future: The role of the oral–microbiota–brain axis in aroma release and perception

Xi,

Yu,

et al. 2024

Comp Rev Food Sci Food Safe

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The field of aroma release and perception during the oral process has been well studied. However, the traditional approaches have not fully explored the integration of oral biology, microbiology, and neurology to further understand aroma release and perception mechanisms. Herein, to address the existing challenges in this field, we introduce the oral–microbiota–brain axis (OMBA), an innovative framework that encapsulates the interactive relationships among saliva and the oral mucosa, the oral microbiota, and the brain in aroma release and perception. This review introduces the OMBA and highlights its role as a key interface facilitating the sensory experience of aroma. Based on a comprehensive literature survey, the specific roles of the oral mucosa, oral microbiota, saliva, and brain in the OMBA are discussed. This integrated approach reveals the importance of each component and the interconnected relationships within this axis in the overall process of aroma release and perception. Saliva and the oral mucosa play fundamental roles in aroma release and perception; the oral microbiota regulates aroma release and impacts olfactory perception; and the brain's intricate neural circuitry is central to the decoding and interpretation of aroma signals. The components of this axis are interdependent, and imbalances can disrupt aroma perception. The OMBA framework not only enhances our comprehension of aroma release and perception but also paves the way for innovative applications that could heighten sensory experiences.

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“…Biological or biomimetic receptor components, such as whole animals, [ 31 ] insect tentacles, [ 32 ] ORs, [ 33 ] odorant‐binding protein (OBP), [ 34 ] peptides, [ 35 ] olfactory cells and tissues, [ 36 ] molecularly imprinted polymers (MIPs), [ 37 ] as the element of the biosensors, allow a significant improvement of selectivity and specificity with simultaneous reduction of the problems associated with cross‐reactivity and complex sample matrix. The secondary transducers are non‐biological devices, which are used to convert and amplify biological signals.…”

Section: Development Of Bioelectronic Nosementioning

confidence: 99%

Artificial Olfactory Biohybrid System: An Evolving Sense of Smell

Qin

Wang

et al. 2022

Advanced Science

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The olfactory system can detect and recognize tens of thousands of volatile organic compounds (VOCs) at low concentrations in complex environments. Bioelectronic nose (B-EN), which mimics olfactory systems, is becoming an emerging sensing technology for identifying VOCs with sensitivity and specificity. B-ENs integrate electronic sensors with bioreceptors and pattern recognition technologies to enable medical diagnosis, public security, environmental monitoring, and food safety. However, there is currently no commercially available B-EN on the market. Apart from the high selectivity and sensitivity necessary for volatile organic compound analysis, commercial B-ENs must overcome issues impacting sensor operation and other problems associated with odor localization. The emergence of nanotechnology has provided a novel research concept for addressing these problems. In this work, the structure and operational mechanisms of biomimetic olfactory systems are discussed, with an emphasis on the development and immobilization of materials. Various biosensor applications and current developments are reviewed. Challenges and opportunities for fulfilling the potential of artificial olfactory biohybrid systems in fundamental and practical research are investigated in greater depth.

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Biotechnological applications of mammalian odorant-binding proteins

Cited by 13 publications

References 96 publications

Ligand Binding Properties of Odorant-Binding Protein OBP5 from Mus musculus

Ligand Binding Properties of Odorant-Binding Protein OBP5 from Mus musculus

The coming future: The role of the oral–microbiota–brain axis in aroma release and perception

Artificial Olfactory Biohybrid System: An Evolving Sense of Smell

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