Biotechnological applications of fiber-optic sensing: multiple uses of a fiber-optic fluorimeter

Anders, K.; Wehnert, G.; Thordsen, O.; Scheper, T.; Rehr, Bert; Sahm, Hermann

doi:10.1016/0925-4005(93)85279-j

Cited by 22 publications

(12 citation statements)

References 28 publications

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“…appl. of fiber sensors: multiple uses of a fiber-optic fluorimeter [36] Optical sensors for bioprocess control [145] Applied biosensors (review with 42 refs.) [168] Real-time biomass concentration monitoring in animal cell cultures [248] Two FIA-based biosensor systems studied for bioprocess monitoring [390] Optical sensors for biotechnological applications [391 ] Sensors for monitoring biotechnological processes [389] [26] Sensors with frequency output, stimulated Brillouin scattering in fibers [63] Review of emitters and detectors for optical gas and chemical sensing [117] Distributed optical fiber sensors (49 refs.…”

Section: Generalmentioning

confidence: 99%

Opto-Chemical and Opto-Immuno Sensors

Gauglitz¹

1996

Sensors Update

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Section: Generalmentioning

confidence: 99%

Opto-Chemical and Opto-Immuno Sensors

Gauglitz¹

1996

Sensors Update

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“…Many important molecules for bioprocesses monitoring and control fluorescence. This includes proteins with aromatic amino acids (tryptophan), NAD(P)H (biomass), adenosinetriphosphate, pyruvate, vitamins, pyridoxines, coenzymes, and flavins [89,116,[127][128][129][130][131]. After excitation of these fluorophores with light of a specific frequency, molecules are elevated to a higher energy level.…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Sensor systems for bioprocess monitoring

Biechele

Busse

Solle

et al. 2015

Engineering in Life Sciences

168

158

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The ability to measure all process variables is of great importance in the field of bioprocess monitoring and control, and continuous, real‐time measurements are highly desired. The more complete and real‐time the measurements are, the more stable, reproducible, and efficient the process can be, leading to reproducibly high‐quality products. This additional information allows the operator to better document the entire process. The process analytical technologies initiative of the US Food and Drug Administration is strongly related to the analysis and control of biopharmaceutical processes. The aim of the initiative is to create processes, generating products of ensured quality by measuring quality‐related process variables. The quality of the product is enhanced by a deep understanding of the process, which is enabled by an effective and suitable sensor system. The aim of this review is to provide an overview of current and emerging sensors for bioprocess monitoring. Sensors directly interfaced to bioreactors for measuring important variables from the gas phase, such as oxygen and carbon dioxide concentration, are discussed, as well as sensors for the monitoring of the biomass concentration and morphology and of the changing medium composition. Furthermore, sensor systems are discussed. These involve sensors (especially biosensors) that are not implemented directly inside the bioreactor but rather are used in conjunction with sample‐taking systems such as flow injection analysis. A major focus is given to spectroscopic sensors, which are noninvasive and offer interesting options for a simultaneous analysis of various compounds. Since data handling is extremely important for this kind of sensor, chemometrics are also included. Soft sensors are discussed as technology that allows a user to incorporate more process data as it become available. Finally, the current state of disposable sensor technology is presented. These sensors are needed for the growing area of disposable and continuous biomanufacturing.

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“…Process-relevant molecules like proteins with aromatic amino acids, NAD(P)H (correlated with biomass), ATP, pyruvate, vitamins, pyridoxines, coenzymes, and flavins are fluorescent [101,[107][108][109][110][111]. Each fluorescent compound has a specific pair of excitation and emission wavelengths at which the signal is maximized, and these are easily detected through an optical window.…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Sensors for disposable bioreactors

Busse

Biechele

Vries

et al. 2017

Engineering in Life Sciences

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Modern bioprocess monitoring demands sensors that provide on-line information about the process state. In particular, sensors for monitoring bioprocesses carried out in single-use bioreactors are needed because disposable systems are becoming increasingly important for biotechnological applications. Requirements for the sensors used in these single-use bioreactors are different than those used in classical reusable bioreactors. For example, long lifetime or resistance to steam and cleaning procedures are less crucial factors, while a requirement of sensors for disposable bioreactors is a cost that is reasonable on a per-use basis. Here, we present an overview of current and emerging sensors for single-use bioreactors, organized by the type of interface of the sensor systems to the bioreactor. A major focus is on non-invasive, in-situ sensors that are based on electromagnetic, semiconducting, optical, or ultrasonic measurements. In addition, new technologies like radio-frequency identification sensors or free-floating sensor spheres are presented. Notably, at this time there is no standard interface between single-use bioreactors and the sensors discussed here.In the future, manufacturers should address this shortcoming to promote single-use bioprocess monitoring and control.

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Biotechnological applications of fiber-optic sensing: multiple uses of a fiber-optic fluorimeter

Cited by 22 publications

References 28 publications

Opto-Chemical and Opto-Immuno Sensors

Opto-Chemical and Opto-Immuno Sensors

Sensor systems for bioprocess monitoring

Sensors for disposable bioreactors

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