“…They have been used as sensitive tools for monitoring gene expression, protein localization, and protein-protein interactions, detection of infections, monitoring of cell death and apoptosis, tumor growth and metastasis in whole animals [31,32], reporter gene assays in a wide area of application [33], protein trafficking [32], drug screening [34], and detection of environmental contamination [35,36]. The major advantages of techniques based on bioluminescence are the very low background in biological systems, versatility, noninvasiveness, reproducibility, high rate, and ease of assay performance with high sensitivity and low cost [31][32][33].…”