Biotechnological applications of bioluminescence and chemiluminescence

RODA, A

doi:10.1016/s0167-7799(04)00085-x

Cited by 28 publications

(37 citation statements)

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“…BL and CL are dark field techniques, which means they do not require an excitation source, therefore, reducing the background fluorescence and greatly improving the sensitivity and potential limits of detection (LODs) when used for detection. BL/CL have been applied widely, including in microarrays and nanoarrays, in in vivo imaging ranging from whole animals down to single cells, in numerous biosensors, and as tracers in immunoassays such as enzyme-linked immunosorbent assays (ELISAs) (38)(39)(40). The only major disadvantages of using BL and CL are that multiple wash and reagent addition steps are often required in the "development" of the signal, which can make the analysis time longer, and the user typically has a limited time in which to collect the generated signal as the local substrate is consumed rapidly.…”

Section: Enzyme-generated Luminescencementioning

confidence: 99%

Fluorescence Spectroscopy: Applications in Chemical Biology

Sapsford

Berti²,

Medintz

2008

Wiley Encyclopedia of Chemical Biology

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Fluorescence spectroscopy in all of its variations can be considered among the most powerful types of analysis available to chemical biology. However, to be useful almost all applications are dependent on optimal labeling of biomolecules with a fluorophore and on the appropriate choice of analytical technique. In this article, we examine the applications and contributions of fluorescent spectroscopy to chemical biology in three inter‐related sections. We first examine the properties of the common fluorophores available from many disparate structural and functional classes, which includes a discussion of their individual benefits and liabilities in the context of their application. The available conjugation chemistries used to attach fluorophores to myriad biomolecules are next reviewed. As each class of biomolecule differs in both structure and function, the focus here is on strategies for the specific labeling of different functional groups. Last, some major types of fluorescent spectroscopy and the associated biologic questions and analysis that can be addressed with them are covered briefly.

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Section: Enzyme-generated Luminescencementioning

confidence: 99%

Fluorescence Spectroscopy: Applications in Chemical Biology

Sapsford

Berti²,

Medintz

2008

Wiley Encyclopedia of Chemical Biology

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“…It has been widely applied in various fields, including clinical diagnosis, biotechnology, pharmacology, food safety, and environmental chemistry [1]. Lucigenin (N,N -dimethyl-9,9 -biacridinium dinitrate) is one of the most efficient chemiluminescent agents, as reported by Gleu and Petsch [2].…”

Section: Introductionmentioning

confidence: 99%

Imidazolium-based ionic liquid derivative/CuII complexes as efficient catalysts of the lucigenin chemiluminescence system and its application to H2O2 and glucose detection

et al. 2015

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The effects of six synthetic imidazolium-based ionic liquids (ILs) on the Cu(II)-catalyzed chemiluminescence of lucigenin (Luc-CL) in the pH range 6.0-11 were investigated. Preliminary experiments found that the CL emission was strongly enhanced or inhibited in the presence of the ILs. The degree of enhancement or inhibition of the CL intensity in the presence of each IL was related to the molecular structure of the IL, the medium used, and the pH. The maximum enhancement of the CL intensity was observed at pH 9.0 (amplification factor = 443). This decrease in the pH at which maximum CL enhancement occurred and the substantial signal amplification of the Luc-CL may be related to a strong interaction between Cu(II) and the imidazolium ring of superior ILs at this pH. Additionally, the formation of IL microdomains in semi-aqueous media permitted more solubility of the product yielded by the Luc-CL reaction (N-methylacridone), which could increase the CL intensity. To obtain consistent data on the catalytic efficiency of Cu(II) in the presence of various ILs as well as the corresponding CL emission intensities, fluorescence quantum yields (Φ F) of lucigenin were measured under the same conditions. Comparison of the data pointed to the mechanism that controls the properties of Luc-CL in the presence of the Cu(II)/IL complexes. Based on the catalytic effect of the Cu(II)/IL complex and the measurement of the enzymatically generated H2O2, a novel, simple, and sensitive CL method for determining glucose with a detection limit (LoD) of 6.5 μM was developed. Moreover, this method was satisfactorily applied to the determination of glucose in human serum and urine samples. Graphical Abstract The lucigenin chemiluminescence assay for H2O2 and glucose using imidazolium-based ionic liquid derivatives/Cu(II) complexes as efficient catalysts at pH 9.0.

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“…They have been used as sensitive tools for monitoring gene expression, protein localization, and protein-protein interactions, detection of infections, monitoring of cell death and apoptosis, tumor growth and metastasis in whole animals [31,32], reporter gene assays in a wide area of application [33], protein trafficking [32], drug screening [34], and detection of environmental contamination [35,36]. The major advantages of techniques based on bioluminescence are the very low background in biological systems, versatility, noninvasiveness, reproducibility, high rate, and ease of assay performance with high sensitivity and low cost [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, red-emitting variants of luciferase are also desirable for multiple tagging systems in whole cells as well as for dual-reporter systems [32,33,40]. Sample medium or environmental conditions produce high variability in the response, which is the main negative aspect encountered in an assay using luciferase as a reporter.…”

Section: Introductionmentioning

confidence: 99%

Molecular enigma of multicolor bioluminescence of firefly luciferase

Hosseinkhani

2010

Cell. Mol. Life Sci.

150

138

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Firefly luciferase-catalyzed reaction proceeds via the initial formation of an enzyme-bound luciferyl adenylate intermediate. The chemical origin of the color modulation in firefly bioluminescence has not been understood until recently. The presence of the same luciferin molecule, in combination with various mutated forms of luciferase, can emit light at slightly different wavelengths, ranging from red to yellow to green. A historical perspective of development in understanding of color emission mechanism is presented. To explain the variation in the color of the bioluminescence, different factors have been discussed and five hypotheses proposed for firefly bioluminescence color. On the basis of recent results, light-color modulation mechanism of firefly luciferase propose that the light emitter is the excited singlet state of OL(-) [(1)(OL(-))*], and light emission from (1)(OL(-))* is modulated by the polarity of the active-site environment at the phenol/phenolate terminal of the benzothiazole fragment in oxyluciferin.

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Biotechnological applications of bioluminescence and chemiluminescence

Cited by 28 publications

References 0 publications

Fluorescence Spectroscopy: Applications in Chemical Biology

Fluorescence Spectroscopy: Applications in Chemical Biology

Imidazolium-based ionic liquid derivative/CuII complexes as efficient catalysts of the lucigenin chemiluminescence system and its application to H2O2 and glucose detection

Molecular enigma of multicolor bioluminescence of firefly luciferase

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