Biotech Green Approaches to Unravel the Potential of Residues into Valuable Products

Gudiña, Eduardo J.; Amorim, Cláudia; Braga, Adelaide; Costa, Ângela Maria Araújo; Rodrigues, Joana Lúcia Lima Correia; Silvério, Sara C.; Rodrigues, L. R.

doi:10.1007/978-3-030-42284-4_5

Cited by 5 publications

(2 citation statements)

References 306 publications

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“…Therefore, it is important to develop more sustainable production processes. In the past decades, engineered microorganisms have been demonstrated to be a suitable option to produce numerous compounds with industrially relevant interest [12][13][14][15][16][17] as the production processes are more environmentally friendly and have the potential to become more cost-effective. The use of synthetic biology and metabolic engineering approaches has allowed to construct more efficient strains able to produce heterologous compounds with higher concentrations and yields [18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Heterologous Production of Acrylic Acid: Current Challenges and Perspectives

Rodrigues

2022

SynBio

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Acrylic acid (AA) is a chemical with high market value used in industry to produce diapers, paints, adhesives and coatings, among others. AA available worldwide is chemically produced mostly from petroleum derivatives. Due to its economic relevance, there is presently a need for innovative and sustainable ways to synthesize AA. In the past decade, several semi-biological methods have been developed and consist in the bio-based synthesis of 3-hydroxypropionic acid (3-HP) and its chemical conversion to AA. However, more recently, engineered Escherichia coli was demonstrated to be able to convert glucose or glycerol to AA. Several pathways have been developed that use as precursors glycerol, malonyl-CoA or β-alanine. Some of these pathways produce 3-HP as an intermediate. Nevertheless, the heterologous production of AA is still in its early stages compared, for example, to 3-HP production. So far, only up to 237 mg/L of AA have been produced from glucose using β-alanine as a precursor in fed-batch fermentation. In this review, the advances in the production of AA by engineered microbes, as well as the hurdles hindering high-level production, are discussed. In addition, synthetic biology and metabolic engineering approaches to improving the production of AA in industrial settings are presented.

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Section: Introductionmentioning

confidence: 99%

Heterologous Production of Acrylic Acid: Current Challenges and Perspectives

Rodrigues

2022

SynBio

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“…Metagenomics has emerged as a culture-independent technique that allows exploring the genetic material of whole microbial communities present in a given environment [6]. This technique has been successfully used to identify novel enzymes with promising catalytic activities and some of them have been patented and already translated to the market [7].…”

Section: Introductionmentioning

confidence: 99%

Metagenomic Approaches as a Tool to Unravel Promising Biocatalysts from Natural Resources: Soil and Water

et al. 2022

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Natural resources are considered a promising source of microorganisms responsible for producing biocatalysts with great relevance in several industrial areas. However, a significant fraction of the environmental microorganisms remains unknown or unexploited due to the limitations associated with their cultivation in the laboratory through classical techniques. Metagenomics has emerged as an innovative and strategic approach to explore these unculturable microorganisms through the analysis of DNA extracted from environmental samples. In this review, a detailed discussion is presented on the application of metagenomics to unravel the biotechnological potential of natural resources for the discovery of promising biocatalysts. An extensive bibliographic survey was carried out between 2010 and 2021, covering diverse metagenomic studies using soil and/or water samples from different types and locations. The review comprises, for the first time, an overview of the worldwide metagenomic studies performed in soil and water and provides a complete and global vision of the enzyme diversity associated with each specific environment.

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