Biosynthetic Potential of a Novel Antarctic Actinobacterium Marisediminicola antarctica ZS314T Revealed by Genomic Data Mining and Pigment Characterization

Abstract

Rare actinobacterial species are considered as potential resources of new natural products. Marisediminicola antarctica ZS314T is the only type strain of the novel actinobacterial genus Marisediminicola isolated from intertidal sediments in East Antarctica. The strain ZS314T was able to produce reddish orange pigments at low temperatures, showing characteristics of carotenoids. To understand the biosynthetic potential of this strain, the genome was completely sequenced for data mining. The complete genome had 3,352,609 base pairs (bp), much smaller than most genomes of actinomycetes. Five biosynthetic gene clusters (BGCs) were predicted in the genome, including a gene cluster responsible for the biosynthesis of C50 carotenoid, and four additional BGCs of unknown oligosaccharide, salinixanthin, alkylresorcinol derivatives, and NRPS (non-ribosomal peptide synthetase) or amino acid-derived compounds. Further experimental characterization indicated that the strain may produce C.p.450-like carotenoids, supporting the genomic data analysis. A new xanthorhodopsin gene was discovered along with the analysis of the salinixanthin biosynthetic gene cluster. Since little is known about this genus, this work improves our understanding of its biosynthetic potential and provides opportunities for further investigation of natural products and strategies for adaptation to the extreme Antarctic environment.

Investigation of a night soil compost psychrotrophic bacteriumGlutamicibacter arilaitensisLJH19 for its safety, efficient hydrolytic and plant growth-promoting potential

“…The presence carotenoid gene cluster supports the indicative yellow color of the LJH19 colonies. Besides pigmentation, the major function of carotenoids in bacteria is to protect the cell from UV radiations, oxidative damage and modify membrane fluidity (Liao et al, 2019).…”

Natural Pigments of Microbial Origin

“…Indeed, approximately 90% of the biosynthetic gene clusters for secondary metabolites has been observed to be included into these categories (Baltz, 2017). Different recent works include the detection of pigment gene clusters (Liao et al, 2019;Xu et al, 2019;Mandakovic et al, 2020). The genome information given by the industrial strain M. purpureus YY-1, compared with closely related filamentous fungi, showed adaptation to starch-based foods.…”

Marine Terpenoids from Polar Latitudes and Their Potential Applications in Biotechnology

“…Indeed, some pigmented bacteria owe their colors to the presence of carotenoids. The genome mining of Marisediminicola antarctica ZS314T, isolated from intertidal sediments of the cost near the Chinese Antarctic Zhongshan Station in East Antarctica, demonstrated the biosynthetic potential of this orange Actinobacterium in producing carotenoids and their derivatives [ 221 ]. Cellulophaga fucicola strain 416 and Zobellia laminarie 465, yellow and orange pigmented bacteria, respectively [ 98 , 99 ], isolated from Antarctic sea sponges, were found to be resistant to UV-B and UV-C radiation, thanks to the expression of carotenoids isolated and chemically identified by ultra-high-performance liquid chromatography with photodiode array and mass spectrometry detectors.…”