Biosynthetic nanobubbles for targeted gene delivery by focused ultrasound

Tayier, Baihetiya; Deng, Zhiting; Wang, Yu; Wang, Wei; Mu, Yuming; Yan, Fei

doi:10.1039/c9nr03402a

Cited by 56 publications

(35 citation statements)

References 34 publications

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“…Moreover, the cavitation effect of ultrasound increased the permeability of the cell membrane and facilitated the entry of the drug into cells, greatly improving the therapeutic effect of the drugs. [28][29][30][31] The experimental results confirmed that G250-TNBs combined with UTND more significantly inhibited the proliferation of tumor cells and promoted their apoptosis than G250-TNBs alone, and showed a superior effect compared with TEM alone (P<0.05). This phenomenon is consistent with previous studies, 15 that is, UTND can cause the concentrated release of drugs in NBs, thereby improving the anti-proliferative effect of drugs while reducing the influence of drugs on the surrounding normal cells and reducing their side effects.…”

Section: Discussionmentioning

confidence: 61%

<p>G250 Antigen-Targeting Drug-Loaded Nanobubbles Combined with Ultrasound Targeted Nanobubble Destruction: A Potential Novel Treatment for Renal Cell Carcinoma</p>

Wang

et al. 2020

IJN

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Purpose: We intended to design G250 antigen-targeting temsirolimus-loaded nanobubbles (G250-TNBs) based on the targeted drug delivery system and to combine G250-TNBs with ultrasound targeted nanobubble destruction (UTND) to achieve a synergistic treatment for renal cell carcinoma (RCC). Methods: The filming-rehydration method was combined with mechanical shock and electrostatic interactions to prepare temsirolimus-loaded nanobubbles (TNBs). G250-TNBs were prepared by attaching anti-G250 nanobodies to the surface of TNBs using the biotinstreptavidin-bridge method. The ability of G250-TNBs to target the G250 antigen of RCC cells and the synergistic efficacy of G250-TNBs and UTND in the treatment of RCC were assessed. Results: The average diameter of the prepared G250-TNBs was 368.7 ± 43.4 nm, the encapsulation efficiency was 68.59% ± 5.43%, and the loading efficiency was 5.23% ± 0.91%. In vitro experiments showed that the affinity of G250-TNBs to the human RCC 786-O cells was significantly higher than that of TNBs (P <0.05), and the inhibitory effect on 786-O cell proliferation and the induction of 786-O cell apoptosis was significantly enhanced in the group treated with G250-TNBs and UTND (G250-TNBs+ UTND group) compared with the other groups (P <0.05). In a nude mouse xenograft model, compared with TNBs, G250-TNBs could target the transplanted tumors and thus significantly enhance the ultrasound imaging of the tumors. Compared with all other groups, the G250-TNBs+UTND group exhibited a significantly lower tumor volume, a higher tumor growth inhibition rate, and a higher apoptosis index (P <0.05). Conclusion: The combined G250-TNBs and UTND treatment can deliver anti-tumor drugs to local areas of RCC, increase the local effective drug concentration, and enhance antitumor efficacy, thus providing a potential novel method for targeted therapy of RCC.

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Section: Discussionmentioning

confidence: 61%

<p>G250 Antigen-Targeting Drug-Loaded Nanobubbles Combined with Ultrasound Targeted Nanobubble Destruction: A Potential Novel Treatment for Renal Cell Carcinoma</p>

Wang

et al. 2020

IJN

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“… 99 , 102 Because of their inherent advantageous physicochemical features and their facile production methods, as well as their ability to disrupt the blood–brain barrier (BBB) discovered in recent years, 103 NBs have received widespread attention especially in the biomedical field. 18 Their biomedical applications include nanomotor-based machinery, 104 drug delivery, 23 , 105 gene delivery, 106 cancer immunotherapy and chemotherapy, 107 , 108 and wound-healing and tissue regeneration. 19 …”

Section: Nbs: Formation and Stabilitymentioning

confidence: 99%

Review of Oxygenation with Nanobubbles: Possible Treatment for Hypoxic COVID-19 Patients

Afshari

Akhavan

Hamblin

et al. 2021

ACS Appl. Nano Mater.

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The coronavirus disease (COVID-19) pandemic, which has spread around the world, caused the death of many affected patients, partly because of the lack of oxygen arising from impaired respiration or blood circulation. Thus, maintaining an appropriate level of oxygen in the patients’ blood by devising alternatives to ventilator systems is a top priority goal for clinicians. The present review highlights the ever-increasing application of nanobubbles (NBs), miniature gaseous vesicles, for the oxygenation of hypoxic patients. Oxygen-containing NBs can exert a range of beneficial physiologic and pharmacologic effects that include tissue oxygenation, as well as tissue repair mechanisms, antiinflammatory properties, and antibacterial activity. In this review, we provide a comprehensive survey of the application of oxygen-containing NBs, with a primary focus on the development of intravenous platforms. The multimodal functions of oxygen-carrying NBs, including antimicrobial, antiinflammatory, drug carrying, and the promotion of wound healing are discussed, including the benefits and challenges of using NBs as a treatment for patients with acute hypoxemic respiratory failure, particularly due to COVID-19.

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“…Due to their very small size, they are able to traverse intercellular gaps and deposit deep into tissues, perhaps making them less desirable to endothelial transfection. They are desirable for applications such as tumor targeting, where they are able to infiltrate deep into the tumor for treatment [168]. They were also shown to be safer to cells than microbubbles, causing transfection in an in vitro model without impacting cell viability [169].…”

Section: Optimizing Bubble Size and Charge For Deliverymentioning

confidence: 99%

Ultrasound and Microbubbles for Targeted Drug Delivery to the Lung Endothelium in ARDS: Cellular Mechanisms and Therapeutic Opportunities

et al. 2021

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Acute respiratory distress syndrome (ARDS) is characterized by increased permeability of the alveolar–capillary membrane, a thin barrier composed of adjacent monolayers of alveolar epithelial and lung microvascular endothelial cells. This results in pulmonary edema and severe hypoxemia and is a common cause of death after both viral (e.g., SARS-CoV-2) and bacterial pneumonia. The involvement of the lung in ARDS is notoriously heterogeneous, with consolidated and edematous lung abutting aerated, less injured regions. This makes treatment difficult, as most therapeutic approaches preferentially affect the normal lung regions or are distributed indiscriminately to other organs. In this review, we describe the use of thoracic ultrasound and microbubbles (USMB) to deliver therapeutic cargo (drugs, genes) preferentially to severely injured areas of the lung and in particular to the lung endothelium. While USMB has been explored in other organs, it has been under-appreciated in the treatment of lung injury since ultrasound energy is scattered by air. However, this limitation can be harnessed to direct therapy specifically to severely injured lungs. We explore the cellular mechanisms governing USMB and describe various permutations of cargo administration. Lastly, we discuss both the challenges and potential opportunities presented by USMB in the lung as a tool for both therapy and research.

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Biosynthetic nanobubbles for targeted gene delivery by focused ultrasound

Abstract: Cationic biosynthetic nanobubbles combined with ultrasound lead to significantly improved gene transfection efficiency.

Cited by 56 publications

References 34 publications

<p>G250 Antigen-Targeting Drug-Loaded Nanobubbles Combined with Ultrasound Targeted Nanobubble Destruction: A Potential Novel Treatment for Renal Cell Carcinoma</p>

<p>G250 Antigen-Targeting Drug-Loaded Nanobubbles Combined with Ultrasound Targeted Nanobubble Destruction: A Potential Novel Treatment for Renal Cell Carcinoma</p>

Review of Oxygenation with Nanobubbles: Possible Treatment for Hypoxic COVID-19 Patients

Ultrasound and Microbubbles for Targeted Drug Delivery to the Lung Endothelium in ARDS: Cellular Mechanisms and Therapeutic Opportunities

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