Biosynthesis, property comparison, and hemocompatibility of bacterial and haloarchaeal poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

Zhao, Youxi; Rao, Zhiming; Xue, Yanfen; Gong, Ping; Ji, Yizhi; Ma, Yupo

doi:10.1007/s11434-015-0923-8

Cited by 16 publications

(9 citation statements)

References 41 publications

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“…That was well within the limit of 5% hemolysis, which is permissible for biomaterials [46]. The hemolysis rate on the PHBV-B film was close to that of the control sample, while it was the lowest for PHBV-H [47].…”

Section: A Study Of Hemocompatible Properties Of Phassupporting

confidence: 70%

The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response

Shishatskaya

Menzyanova

Shumilova

2019

International Journal of Biological Macromolecules

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Section: A Study Of Hemocompatible Properties Of Phassupporting

confidence: 70%

The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response

Shishatskaya

Menzyanova

Shumilova

2019

International Journal of Biological Macromolecules

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“…PHAs concentration and composition were determined using 80 mg dry cell samples and analyzed by gas chromatography (GC). At first, dried cells were subjected to derivatization in a mixture of 2 mL esterified liquid (1 g/L sodium benzoate was used as an internal standard and dissolved in 97% (v/v) methanol and 3% (v/v) concentrated sulfuric acid) and 2 mL chloroform solution at 100°C for 4 h. en, the resulting sample (1 μL) methanolyzed was assayed using GC Clarus 580 (Perki-nElmer, USA) equipped with a BD-FFAP column (30 m × 0.32 nm × 0.25 μm) [26]. Nitrogen was separation carrier gas (2 mL/min).…”

Section: Gc and Gc-ms Analysismentioning

confidence: 99%

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production by Rhodospirillum rubrum Using a Two-Step Culture Strategy

Liu

Zhao

Diao

et al. 2019

Journal of Chemistry

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Polyhydroxyalkanoates (PHAs) are microbially synthesized biopolyesters which have attracted great attentions as a new biological material, potential alternative to traditional fossil fuel-based plastic due to their biodegradability and biocompatibility. Poly-3-hydroxybutyrate (PHB) and poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are the most common members of PHAs. In this study, the nonsulfur and facultatively phototrophic bacterium Rhodospirillum rubrum was cultivated to accumulate PHA by a two-step culture strategy. Gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), and nuclear magnetic resonance spectroscopy (NMR) analyses showed that PHAs synthesized from fructose was PHBV, in which the 3HV content was 46.5 mol%, which means the better mechanical property. The molecular weight, distribution, and thermal features were characterized by gel permeation chromatography (GPC), differential scanning calorimeter (DSC), and thermo gravimetric analysis (TGA), respectively. The low PDI of 1.08 revealed the narrow and evenly molar mass distribution which shows the stable features. The high melting temperature and their other physical properties implied their potential applications. The traditional process of producing PHBV involves related carbon sources such as valeric acid. However, our study clearly described a new medium formula with fructose and a complete fermentation method to produce PHBV with a high 3HV faction and low molecular distribution.

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“…We found that, after 24 h of exposure, the concentrations of molecular markers were significantly different from those of the blank control for rabbit and human blood. The increase in the concentrations of molecular markers may be related to the physical and chemical properties of the surface of the materials [21][22][23], including surface roughness and molecular structure (Fig. 1).…”

Section: Discussionmentioning

confidence: 99%

Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices

Wang

et al. 2020

Thrombosis Research

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Biosynthesis, property comparison, and hemocompatibility of bacterial and haloarchaeal poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

Cited by 16 publications

References 41 publications

The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response

The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production by Rhodospirillum rubrum Using a Two-Step Culture Strategy

Feasibility study of use of rabbit blood to evaluate platelet activation by medical devices

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