Biosynthesis, production and applications of bacterial cellulose

Lin, Shin Ping; Loira, Iris; Catchmark, Jeffrey M.; Liu, Je-Ruei; Demirci, Ali; Cheng, Kuan‐Chen

doi:10.1007/s10570-013-9994-3

Cited by 396 publications

(212 citation statements)

References 225 publications

Supporting

Mentioning

204

Contrasting

Unclassified

Order By: Relevance

“…Medium pH was found to be critical for high BC productivity in many studies [10,11]. Obviously, gluconic acid reduces the pH of the medium down to 3.0, which can result in inhibition of BC accumulation.…”

Section: Discussionmentioning

confidence: 99%

“…Analysis of known results enables determination of several main problems of BC production in general. It is a rather long process (up to 336 h), and the final product yield is influenced by many factors (type and concentration of the carbon source in the medium, composition and pH of the nutrient medium) [11]. Various carbon sources were examined for the purpose of BC synthesis, while glucose appeared to be the high-usage carbon source for the synthesis of BC [12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

“Deceived” Concentrated Immobilized Cells as Biocatalyst for Intensive Bacterial Cellulose Production from Various Sources

Stepanov

Ефременко

2018

Catalysts

View full text Add to dashboard Cite

Abstract:A new biocatalyst in the form of Komagataeibacter xylinum B-12429 cells immobilized in poly(vinyl alcohol) cryogel for production of bacterial cellulose was demonstrated. Normally, the increased bacteria concentration causes an enlarged bacterial cellulose synthesis while cells push the polysaccharide out to pack themselves into this polymer and go into a stasis. Immobilization of cells into the poly(vinyl alcohol) cryogel allowed "deceiving" them: bacteria producing cellulose pushed it out, which further passed through the pores of cryogel matrix and was accumulated in the medium while not covering the cells; hence, the latter were deprived of a possible transition to inactivity and worked on the synthesis of bacterial cellulose even more actively. The repeated use of immobilized cells retaining 100% of their metabolic activity for at least 10 working cycles (60 days) was performed. The immobilized cells produce bacterial cellulose with crystallinity and porosity similar to polysaccharide of free cells, but having improved stiffness and tensile strength. Various media containing sugars and glycerol, based on hydrolysates of renewable biomass sources (aspen, Jerusalem artichoke, rice straw, microalgae) were successfully applied for bacterial cellulose production by immobilized cells, and the level of polysaccharide accumulation was 1.3-1.8-times greater than suspended cells could produce.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“Deceived” Concentrated Immobilized Cells as Biocatalyst for Intensive Bacterial Cellulose Production from Various Sources

Stepanov

Ефременко

2018

Catalysts

View full text Add to dashboard Cite

show abstract

“…Cellulose is widely utilized in industrial applications (textiles, dietary supplements, gauze, scaffolds for tissue regeneration, and even headphones) (72,73). Microbial cellulose is coveted because of its purity and is typically harvested from Gluconacetobacter xylinus (73,74). One of the largest problems with the industrial production of microbial cellulose from G. xylinus is the high production costs (74).…”

Section: Discussionmentioning

confidence: 99%

“…Microbial cellulose is coveted because of its purity and is typically harvested from Gluconacetobacter xylinus (73,74). One of the largest problems with the industrial production of microbial cellulose from G. xylinus is the high production costs (74). Glucose-based medium is used for G. xylinus growth and cellulose production, and the metabolic by-products produced during growth actually inhibit cellulose production (74).…”

Section: Discussionmentioning

confidence: 99%

The Disulfide Bonding System Suppresses CsgD-Independent Cellulose Production in Escherichia coli

2014

View full text Add to dashboard Cite

The bacterial extracellular matrix encases cells and protects them from host-related and environmental insults. The Escherichia coli master biofilm regulator CsgD is required for the production of the matrix components curli and cellulose. CsgD activates the diguanylate cyclase AdrA, which in turn stimulates cellulose production through cyclic di-GMP (c-di-GMP). Here, we identified and characterized a CsgD-and AdrA-independent cellulose production pathway that was maximally active when cultures were grown under reducing conditions or when the disulfide bonding system (DSB) was compromised. The CsgD-independent cellulose activation pathway was dependent on a second diguanylate cyclase, called YfiN. c-di-GMP production by YfiN was repressed by the periplasmic protein YfiR, and deletion of yfiR promoted CsgD-independent cellulose production. Conversely, when YfiR was overexpressed, cellulose production was decreased. Finally, we found that YfiR was oxidized by DsbA and that intraprotein YfiR disulfide bonds stabilized YfiR in the periplasm. Altogether, we showed that reducing conditions and mutations in the DSB system caused hyperactivation of YfiN and subsequent CsgD-independent cellulose production.

show abstract

“…However, the relatively high cost of BC production limits its application in the production of high value-added products and specialty chemicals 7, 10, 11 . The yield of a BC biosynthesis process depends on such factors as temperature, ratio of the surface area to the volume of the substrate and on the time of synthesis 12, 13 .…”

Section: Introductionmentioning

confidence: 99%

Effect ofGluconacetobacter xylinuscultivation conditions on the selected properties of bacterial cellulose

Fijałkowski

Żywicka

Drozd

et al. 2016

Polish Journal of Chemical Technology

View full text Add to dashboard Cite

The aim of the study was to analyze the changes in the parameters of bacterial cultures and bacterial cellulose (BC) synthesized by four reference strains of Gluconacetobacter xylinus during 31-day cultivation in stationary conditions. The study showed that the most visible changes in the analyzed parameters of BC, regardless of the bacterial strain used for their synthesis, were observed in the fi rst 10-14 days of the experiment. It was also revealed, that among parameters showing dependence associated with the particular bacterial strain were the rate and period of BC synthesis, the growth rate of bacteria anchored to the cellulose fi brils, the capacity to absorb water and the water release rate. The results presented in this work may be useful in the selection of optimum culturing conditions and period from the point of view of good effi ciency of the cellulose synthesis process.

show abstract

Biosynthesis, production and applications of bacterial cellulose

Cited by 396 publications

References 225 publications

“Deceived” Concentrated Immobilized Cells as Biocatalyst for Intensive Bacterial Cellulose Production from Various Sources

“Deceived” Concentrated Immobilized Cells as Biocatalyst for Intensive Bacterial Cellulose Production from Various Sources

The Disulfide Bonding System Suppresses CsgD-Independent Cellulose Production in Escherichia coli

Effect ofGluconacetobacter xylinuscultivation conditions on the selected properties of bacterial cellulose

Contact Info

Product

Resources

About