Biosynthesis of ZnO nanoparticles using Jacaranda mimosifolia flowers extract: Synergistic antibacterial activity and molecular simulated facet specific adsorption studies

Abstract

The naturally occurring biomolecules present in the plant extracts have been identified to play an active role in the single step formation of nanoparticles with varied morphologies and sizes which is greener and environmentally benign. In the present work, spherical zinc oxide nanoparticles (ZnO NPs) of 2-4nm size were synthesized using aqueous extract of fallen Jacaranda mimosifolia flowers (JMFs), treated as waste. The microwave assisted synthesis was completed successfully within 5min. Thereafter, phase identification, morphology and optical band gap of the synthesized ZnO NPs were done using X-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM) and UV-Visible spectroscopy techniques. The composition of JMFs extract was analyzed by gas chromatography-mass spectrometry (GC-MS) and the ZnO NPs confirmation was further explored with fourier transform infrared spectroscopy (FTIR). The GC-MS results confirmed the presence of oleic acid which has high propensity of acting as a reducing and capping agent. The UV-Visible data suggested an optical band gap of 4.03eV for ZnO NPs indicating their small size due to quantum confinement. Further, facet specific adsorption of oleic acid on the surface of ZnO NPs was studied computationally to find out the impact of biomolecules in defining the shape and size of NPs. The viability of gram negative Escherichia coli and gram positive Enterococcus faecium bacteria was found to be 48% and 43%, respectively at high concentration of NPs.

The Study of Structural, Physical and Electrochemical Activity of Zno Nanoparticles Synthesized by Green Natural Extracts of Sageretia Thea

“…Since bacterial pathogens have been found to develop resistance against antibiotics [6, 19,20]. There are only a few reports demonstrating antibacterial activity of ZnO nanoparticles against multiple-antibiotic-resistant (MAR) strains [21]. The first evidence of ZnO action against multidrug resistance S. aureus came, found that the killing ability of zinc oxide against multidrug resistance cells increased in the presence of some common antibiotics such as ciprofloxacillin [6,20,21].…”

“…There are only a few reports demonstrating antibacterial activity of ZnO nanoparticles against multiple-antibiotic-resistant (MAR) strains [21]. The first evidence of ZnO action against multidrug resistance S. aureus came, found that the killing ability of zinc oxide against multidrug resistance cells increased in the presence of some common antibiotics such as ciprofloxacillin [6,20,21]. Although the combination therapy is found useful against multidrug resistance bacteria, the exact mechanism of nanoparticle action still remains ambiguous.…”

“…The mechanism for bactericidal property of ZnO has been described to be the production of Reactive Oxygen Species (ROS) that internalize the bacterial cell envelope and the damage it leads to subsequent cell death. A number of studies have indicated that the primary cause of the antibacterial action might be from the disruption of cell membrane [6,[19][20][21].…”

Flower-Based Green Synthesis of Metallic Nanoparticles: Applications beyond Fragrance

“…These AgNPs displayed a better antibacterial activity in both Gram-negative and Gram-positive bacteria than the extract, and the maximum recorded antibacterial activity was against vancomycin-resistant enterococci (Van-A type), i.e., Enterococcus faecium. Sharma et al (2016) synthesized spherical zinc oxide nanoparticles (ZnO NPs), with a size of 2-4 nm, from fallen Jacaranda mimosifolia flower aqueous extract (JMFs) [83]. In GC-MS analysis, the oleic acid in the flower extract was found to act as a reducing and capping agent, and the presence of oleic acid stabilized ZnO NPs, showing antibacterial activity against both Gram-positive E. faecium and Gram-negative E. coli bacteria.…”

New frontiers in the biosynthesis of metal oxide nanoparticles and their environmental applications: an overview

“…The assynthesized nanomaterial were used as antibiotics against multi-drug resistant clinical bacterial isolates. In another study, Sharma and his coworkers [54] used leaf extract of Azadirachta indica (Neem) plant as a reducing agent for Fig. 2 Mechanism of bioreduction of titanyl hydroxide to TiO 2 NPs using Euphorbia heteradena Jaub root extract as bio-template [51].…”