Biosynthesis of zinc oxide nanoparticles using Fumaria parviflora extract and evaluation of their antibacterial and antioxidant activities

Abstract

Green synthesis is a biocompatible and stable method of producing zinc oxide nanoparticles (ZnONPs).In the present study, ZnONPs were biosynthesized using Fumaria parviflora extract by the green method, and the antibacterial and antioxidant properties of these NPs were evaluated. The characteristics of the synthesized ZnONPs were determined by ultraviolet-visible spectrophotometry (UV-VIS), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The antioxidant activity of the NPs was tested by the α-diphenyl-β-picrylhydrazyl (DPPH) method. Antibacterial properties of the synthesized ZnONPs were evaluated against Staphylococcus aureus and Escherichia coli by disc diffusion and microdilution methods. The results of UV-VIS spectroscopy revealed an absorption peak at 370 nm. XRD results showed the formation of a hexagonal wurtzite structure, and SEM analyses demonstrated that ZnONPs had a spherical shape with an average size 42 to 60 nm. Free radical scavenging capacity of ZnONPs was assessed using the DPPH assay with varying concentrations of ZnONPs, and scavenging activity was observed with IC 50 of 30.86 μg/ml. In the antibacterial assay, the inhibition zone of the synthesized NPs at 100 μg/ml concentration for S. aureus (24.6 ± 0.72) was greater than that of the antibiotics vancomycin (23 ± 0.51) and a mikacin (13 ± 0.40) and was greater for E. coli (13.2 ± 0.81)than that of vancomycin (12 ± 0.41) ( P ≤ 0.05). The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of ZnONPs for S. aureus and E. coli were 1.56 and 3.125 μg/ml, and 6.25 and 12.5 μg/ml, respectively. The biosynthesized ZnONPs showed strong antibacterial and antioxidant activities.

Antibacterial potential of biosynthesized silver nanoparticles using Nepeta sessilifolia Bunge and Salvia hydrangea DC. ex Benth. extracts from the natural habitats of Iran's Rangelands

“…The maximum absorption peak in the range of 400 to 450 nm indicates the synthesis of silver nanoparticles. It is related to the surface plasmon resonance of silver nanoparticles, which is related to the induction of free electrons in the nanoparticles [ 65 ].

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Effects of Titanium Dioxide Nanoparticles and Coenzyme Q10 on Testicular Ischemia-Reperfusion Injury: Role of the Mitochondrial Apoptosis Pathway

“…The biosynthesis of NPs through plants is a proper biocompatible method that is under consideration by researchers [27]. Numerous studies have been performed on the antioxidant properties of NPs and their ability to reduce apoptosis.…”

Comparative Evaluation of Biogenesis of ZnO Nanoparticles Using Leaf Extracts of Bacopa monnieri, Acacia arabica and Catharanthus roseus

“…Zinc oxide nanoparticles (ZnONPs) have been widely used in various fields such as electronics, optoelectronics, biomedical, textile, cosmetics, water treatment, food packaging, and agriculture applications (1,2) . In chemical-based methods for the synthesis of ZnONPs typically involve the use of toxic chemicals, solvents, and https://www.indjst.org/ stabilizers, which are harmful for human health and the environment (3) .…”