Biosynthesis of value-added bioproducts from hemicellulose of biomass through microbial metabolic engineering

Geng, Biao; Jia, Xiuyi; Peng, Xiaowei; Han, Young-Tak

doi:10.1016/j.mec.2022.e00211

Cited by 8 publications

(2 citation statements)

References 152 publications

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“…A possible explanation for this problem might be the limited expression levels of galactose metabolic genes in the engineered strain during acetoin fermentation. On the other hand, the arabinose transporter of Bacillus subtilis has different affinities for arabinose, xylose, and galactose, and this may cause the galactose uptake rate to be lower than xylose and arabinose (Geng et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Liu

Guo

et al. 2023

AMB Expr

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Okara, a renewable biomass resource, is a promising fermentative raw material for the bio-production of value-added chemicals due to its abundance and low-costs. we developed a process for the enzymatic hydrolysis of okara, and then engineered Bacillus subtilis to utilize mixed sugars to produce acetoin in okara hydrolysis without the addition of a supplemental nitrogen source. Okara was initially hydrolyzed with cellulase, β-glucosidase, and pectinase to obtain okara hydrolysate containing mixed sugars (32.78 ± 0.23 g/L glucose, 1.43 ± 0.064 g/L arabinose, 7.74 ± 0.11 g/L galactose) and amino acids. In this study, Bacillus subtilis 168 was used as the acetoin-producing strain, and the key genes bdhA and acoA of the acetoin catabolism pathway were knocked out to improve the fermentation yield of acetoin. In order to utilize the galactose in the hydrolysate, the recombinant strain BS03 (Bacillus subtilis168∆bdhA∆acoA) was used to overexpress the arabinose transporter-encoding gene (araE) drive heterologous expression of the Leloir pathway gene (galKTE). The corn dry powder concentration was optimized to 29 g/L in the reducing sugar okara hydrolysate. The results show that the recombinant bacterium BS03 could still synthesize 11.79 g/L acetoin without using corn dry powder as a nitrogen source. Finally, using okara enzymatic hydrolysate as the carbon and nitrogen source, 11.11 g/L and 29.7 g/L acetoin were obtained by batch fermentation and fed-batch fermentation, respectively, which was further converted to 5.33 g/L and 13.37 g/L tetramethylpyrazine (TTMP) by reaction with an ammonium salt.

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Section: Discussionmentioning

confidence: 99%

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Liu

Guo

et al. 2023

AMB Expr

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“…Recent decades have seen a substantial expansion in using microorganisms to produce fuels and chemicals derived from renewable resources [1][2][3]. This trend reflects mounting concerns regarding global warming and the limited availability of fossil fuels [4,5].…”

Section: Introductionmentioning

confidence: 99%

Hydroxybenzoic Acid Production Using Metabolically Engineered Corynebacterium glutamicum

Doke¹,

Kishida²,

Hirata³

et al. 2023

Synthetic Biology and Engineering

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Hydroxybenzoic acids (HBAs), including 4-HBA, 3-HBA, and 2-HBA, are valuable platform chemicals for production of commodity materials and fine chemicals. Herein, we employed metabolic engineering techniques to enhance the production of these HBAs in Corynebacterium glutamicum ATCC 13032. Our approach augmented the shikimate pathway and eliminated genes associated with HBA degradation, particularly phenol 2-monooxygenase encoded by cg2966. Increased titers of 3-HBA and 4-HBA were achieved via selection of suitable promoters for 3-hydroxybenzoate synthase and chorismate pyruvate lyase. A tac-M1 promoter was suitable for chorismate pyruvate lyase expression and 8.3 g/L of 4-HBA production was achieved. Efficient production of 2-HBA was enabled by maintaining a balanced expression of isochorismate synthase and isochorismate pyruvate lyase. Consequently, strains KSD5-tacM1-H and KSD5-J2-PE exhibited production levels of 19.2 g/L of 3-HBA and 12.9 g/L of 2-HBA, respectively, using 1 L jar fermenter containing 80 g/L of glucose. Therefore, this engineered strain platform holds significant potential for production of other valuable products derived from chorismate.

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Utilizing fungal biodegradation for valorisation of lignocellulosic waste biomass and its diverse applications

Mehta,

Chelike

2024

Applied Research

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Myco degradation is an effective technique for breaking down waste plant substances made of lignin, cellulose, and hemicellulose, which are collectively known as lignocellulose. This abundant organic material is found throughout the world. Due to its recalcitrant nature, lignocellulose poses a challenge for efficient conversion into biofuels, biochemicals, and other valuable products. Myco degradation, which involves the use of fungi to degrade lignocellulosic materials, offers a sustainable and cost‐efficient resolution to this challenge. This review provides an overview of the mechanisms and applications of myco degradation for lignocellulosic biomass degradation. The review discusses the various types of fungi involved in lignocellulose degradation, their enzymatic systems, and the factors that influences their performance. Furthermore, the potential applications of myco degradation products, such as biofuels, enzymes, and bioplastics, are reviewed. It also highlights the implications of myco degradation for waste management and sustainable development. Overall, myco degradation represents a promising technology for the efficient deprivation of lignocellulosic waste biomass, and further research in this field holds great potential for the sustainable creation of bio‐based products.This article is protected by copyright. All rights reserved.

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Biosynthesis of value-added bioproducts from hemicellulose of biomass through microbial metabolic engineering

Cited by 8 publications

References 152 publications

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Hydroxybenzoic Acid Production Using Metabolically Engineered Corynebacterium glutamicum

Utilizing fungal biodegradation for valorisation of lignocellulosic waste biomass and its diverse applications

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