Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY

Melamed, Jacob; Kocev, Alexander; Торгов, В. И.; Veselovsky, V. V.; Brockhausen, Inka

doi:10.1007/s10719-022-10040-4

“…L-3-cyano-alanine, a non-proteinogenic cyanoamino acid produced by bacteria to detoxify cyanide from endogenous and exogenous sources, was also found in the DSS lymph. 76 Several components of the bacterial wall were also found in the afferent lymph following DSS-mediated damage including mucic acid, 77 rhamnose, 78 and β- d -mannosyl phosphomycoketide, a glycolipid known to activate immune cells through CD1d binding. Also elevated was methylglutaric acid, which induces lipid peroxidation and mitochondrial disfunction 79 …”

Section: Discussionmentioning

confidence: 99%

Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load

Nanaware,

Khan,

Clement

et al. 2024

Cell Reports

0

View full text Add to dashboard Cite

“…The appearance of these biofilms suggests that in these mutants, related cells are fully dispersed from each other. Finally, ∆ssg and ∆wapR-mutants that are predicted to form LPS without the O-antigen attached (Fig 3B and Fig S1) [71][72][73]--gave rise to a "clustered" phenotype. These biofilms showed random cell orientation, but fluorescent cells also formed clusters with vertical elongation in zones 2 and 3, indicating that these mutants retained some ability to arrange related cells along the y-axis (Fig 3B and Fig S1).…”

Section: Resource Availability Affects the Organization Of Cellular-a...mentioning

confidence: 99%

Cell arrangement impacts metabolic activity and antibiotic tolerance inPseudomonas aeruginosabiofilms

Dayton¹,

Kiss²,

Wei³

et al. 2023

Preprint

View full text Add to dashboard Cite

Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than they would via diffusion. Microbes growing in multicellular structures, called biofilms, are also affected by differential access to resources and we hypothesized that this is influenced by the physical arrangement of the cells. In this study, we examined the microanatomy of biofilms formed by the pathogenic bacterium Pseudomonas aeruginosa and discovered that clonal cells form striations that are packed lengthwise across most of a mature biofilm’s depth. We identified mutants, including those defective in pilus function and in O-antigen attachment, that show alterations to this lengthwise packing phenotype. Consistent with the notion that cellular arrangement affects access to resources within the biofilm, we found that while the wild type shows even distribution of tested substrates across depth, the mutants show accumulation of substrates at the biofilm boundaries. Furthermore, we found that altered cellular arrangement within biofilms affects the localization of metabolic activity, the survival of resident cells, and the susceptibility of subpopulations to antibiotic treatment. Our observations provide insight into cellular features that determine biofilm microanatomy, with consequences for physiological differentiation and drug sensitivity.

show abstract

“…The appearance of these biofilms suggests that in these mutants, related cells are fully dispersed from each other. Finally, Δssg and ΔwapRmutants that are predicted to form LPS without the O-antigen attached (Figs 3B and S5) [90][91][92]-gave rise to a "clustered" phenotype. These biofilms showed random cell orientation, but fluorescent cells also formed clusters with vertical elongation in zones 2 and 3, indicating that these mutants retained some ability to restrict movement of related cells in the x-y plane (Figs 3B and S5).…”

Section: Global Regulators and Cell Surface Components Affect Cell Pa...mentioning

confidence: 99%

Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms

Dayton,

Kiss,

Wei

et al. 2024

PLoS Biol

7

0

View full text Add to dashboard Cite

Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than they would via diffusion. Microbes growing in multicellular structures, called biofilms, are also affected by differential access to resources and we hypothesized that this is influenced by the physical arrangement of the cells. In this study, we examined the microanatomy of biofilms formed by the pathogenic bacterium Pseudomonas aeruginosa and discovered that clonal cells form striations that are packed lengthwise across most of a mature biofilm’s depth. We identified mutants, including those defective in pilus function and in O-antigen attachment, that show alterations to this lengthwise packing phenotype. Consistent with the notion that cellular arrangement affects access to resources within the biofilm, we found that while the wild type shows even distribution of tested substrates across depth, the mutants show accumulation of substrates at the biofilm boundaries. Furthermore, we found that altered cellular arrangement within biofilms affects the localization of metabolic activity, the survival of resident cells, and the susceptibility of subpopulations to antibiotic treatment. Our observations provide insight into cellular features that determine biofilm microanatomy, with consequences for physiological differentiation and drug sensitivity.

show abstract

Biosynthesis of the Pseudomonas aeruginosa common polysaccharide antigen by D-Rhamnosyltransferases WbpX and WbpY

Cited by 10 publications

References 51 publications

Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load

Role of the afferent lymph as an immunological conduit to analyze tissue antigenic and inflammatory load

Cell arrangement impacts metabolic activity and antibiotic tolerance inPseudomonas aeruginosabiofilms

Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms

Contact Info

Product

Resources

About