Biosynthesis of the lantibiotic nisin: genomic organization and membrane localization of the NisB protein

Abstract

Nisin produced by Lactococcus lactis 6F3 is used as a food preservative and is the most important member of a group of peptide-antibiotics containing lanthionine bridges (lantibiotics) (N.

Genetics of bacteriocins produced by lactic acid bacteria

“…Primer extension mapping using nisA probes indicate that nisA and nisB are transcribed as part of a polycistronic operon, which has been proposed to begin at least 4 kb upstream of nisA and end at the rho-independent terminator located behind nisB [102,113]. There is no obvious promoter upstream of niLz4 [113,129] and the 5'-end of nisA transcript was 'ragged', implicating mRNA processing at a site approximately 40-43 bp upstream from the initiation codon of nisA [113]. The 3'-end of the nisA transcript ended at the inverted repeat positioned between nisA and nisB, suggesting that this structure provides a mRNA processing site [105,113,129].…”

“…There is no obvious promoter upstream of niLz4 [113,129] and the 5'-end of nisA transcript was 'ragged', implicating mRNA processing at a site approximately 40-43 bp upstream from the initiation codon of nisA [113]. The 3'-end of the nisA transcript ended at the inverted repeat positioned between nisA and nisB, suggesting that this structure provides a mRNA processing site [105,113,129]. In contrast, Engelke et al [129] concluded that nisAB is transcribed as a monocistronic mRNA from an initiation point just upstream of nisA, and subjected to processing at the IR to form two primary mRNA species of 0.3 kb and 3 kb.…”

“…The 3'-end of the nisA transcript ended at the inverted repeat positioned between nisA and nisB, suggesting that this structure provides a mRNA processing site [105,113,129]. In contrast, Engelke et al [129] concluded that nisAB is transcribed as a monocistronic mRNA from an initiation point just upstream of nisA, and subjected to processing at the IR to form two primary mRNA species of 0.3 kb and 3 kb. The possibility of mRNA processing upstream of nisA was not ruled out since no concensus promoter was found before nisA.…”

Microbial quorum sensing: a tool or a target for antimicrobial therapy?

“…The complex biosynthesis of nisin is mediated by 11 genes organized in a cluster, i.e. nisABTCIPRKFEG (nisA-nisG) [90][91][92][93][94][95]. The first of these genes, nisA, encodes a precursor peptide of 57 amino acid residues called nisin A. Nisin A is post-translationally modified by enzymes encoded by nisB and nisC.…”

Progress and potential in the biotechnology of lactic acid bacteria

“…The prenisin structural gene nisA is followed by a large gene nisB and nisT which has a translocation function. The next gent nisC has no known role, but nisl is involved in immunity, nisP is the leader peptidase and nisR probably has a regulatory role [128][129][130][131][132].…”