Biosynthesis of tamoxifen coated chitosan biopolymer, its characterization, DNA damage study and anticancer study against breast cancer cell line

Abstract

Tamoxifen has a broad spectrum of anticancer activity. The main aim of the study is to determine the biosynthesis of tamoxifen coated chitosan biopolymer which can be characterized by SEM and FTIR. The loading efficiency of oyster shell chitosan shows higher value than that of the shrimp shell chitosan. The ampicillin and E-coli culture were introduced into tamoxifen drug coated with biopolymer results in the isolation of DNA and plasmid. It determines the DNA damage activity of tamoxifen drug coated biopolymer (chitosan). The in vitro drug release study plays an important role in expressing the penetration activity of tamoxifen drug from the dialysis bag into the water and the drug releasing capacity is 48%. DMEM provides enough nutrients for the breast cancer cell line (MCF -7) to grow properly. The mixed proportion (10µl and 20µl) of Tamoxifen drug coated biopolymer introduced into 96 microtitre well plates besides breast cancer cell lines (MCF 7) in CO 2 incubator for 72 hours. Readings taken from ELISA reader shows that Cell death in 10µl sample of oyster shell chitosan coated tamoxifen drug was 59.41%. 10µl sample of oyster shell chitosan coated tamoxifen has shown high anticancer activity in MCF -7 cell lines.