Biosynthesis of silver nanoparticles with antibacterial, antioxidant, anti-inflammatory properties and their burn wound healing efficacy

Abstract

The current study aims to develop a novel burn wound ointment consisting of sheep’s tail ointment loaded with AgNP. The AgNP in the ointment serves as an antibacterial, antioxidant and anti-inflammatory agent. The AgNP was developed via the biological method with the assistance of the medicinal plant Rhodiola rosea. The characterization of AgNP was assessed using UV-Vis spectroscopy, FTIR, Zeta Potential, XRD, PCCS, SEM, and EDX techniques. The formation of AgNP was confirmed by UV-Vis spectrum at the absorbance of ∼430 nm, and the biomolecules responsible for reducing and capping the AgNP were characterized by FTIR analysis. The stability of AgNP was determined with Zeta potential, which revealed a highly stable colloidal solution with a surface charge of −68.38 ± 3.4 mV. The synthesized AgNP had a face-centered cubic structure with a crystallite size of 23 nm and average grain size of 67.5 nm. The SEM image showed a fairly monodisperse 20 nm-sized spherical-shaped AgNP. The synthesized AgNP contained high purity of the silver, and a low concentration of AgNP inhibited both Gram-positive and Gram-negative bacteria. Moreover, the scavenging activity of AgNP was investigated using DPPH and H2O2 scavenging assay, and the results revealed a dose-dependent antioxidant activity with the highest activity at a concentration of 450 μg/ml. Finally, the burn wound healing effect was evaluated by applying the AgNP-loaded ointment to the wound site of BALB/c mice. The in-vivo studies confirmed that AgNP-loaded ointment reduced the wound size, decreased the epidermis layer, and lowered mast cell migration compared to untreated burn wounds. And the synthesized AgNP regulated both pro-inflammatory and anti-inflammatory gene expression, thereby promoting burn wound closure on BALB/c mice. The developed AgNP-loaded ointment has the potential to be applied in the biomedical field.

Exploring the biological application of Penicillium fimorum-derived silver nanoparticles: In vitro physicochemical, antifungal, biofilm inhibitory, antioxidant, anticoagulant, and thrombolytic performance

“…The color change from pale yellow to dark brown following the fabrication of AgNPs was attributed to a phenomenon named surface plasmon resonance (SPR) resulting from the collective oscillation of free electrons of AgNPs in response to light in the visible region. These findings were in accordance with previous studies [ [30] , [31] , [32] ]. The exact mechanism of fungus-mediated synthesis of AgNPs has not been fully understood.…”

“…Other EDX signals were attributed to the X-ray emission of the elements in the structure of the biomolecules that were present in the supernatant and acted as reducing and capping/stabilizing agents. Similar findings were reported in previous studies [ 31 , 32 , 35 ].…”

A systematic review on green synthesis of silver nanoparticles, characterization and applications

“…The treatment involving biosynthesized silver nanoparticles showed promise in modulating the expression of key cytokines, reducing pro-inflammatory signals, and promoting an anti-inflammatory environment, which could be beneficial for the wound healing process after burn injuries (90,91).…”

Viridibacillus culture derived silver nanoparticles exert potent anticancer action in 2D and 3D models of lung cancer via mitochondrial depolarization-mediated apoptosis

“…Owing to this corona, these AgNPs show excellent stability over longer periods of times [ [14] , [15] , [16] , [17] ]. Several reports show that such “green AgNPs” have antibacterial, anti-inflammatory, and anticancer activities [ 18 ]. It has been shown that the AgNPs show antibacterial activity by interacting with bacterial cell membrane, bacterial proteins, and bacterial nucleic acids; whereas, they show anti-inflammatory properties by reducing the expression of pro-inflammatory cytokines such as interleukin-6, interleukin-1β [ 18 ].…”

“…Several reports show that such “green AgNPs” have antibacterial, anti-inflammatory, and anticancer activities [ 18 ]. It has been shown that the AgNPs show antibacterial activity by interacting with bacterial cell membrane, bacterial proteins, and bacterial nucleic acids; whereas, they show anti-inflammatory properties by reducing the expression of pro-inflammatory cytokines such as interleukin-6, interleukin-1β [ 18 ]. According to the extensive review published by Mariana Morais et al the green AgNPs have been proven to be active against breast cancer, lung cancer, head and neck cancer, gynecologic cancer, urologic cancer, digestive system cancer, skin cancer, brain cancer, blood cancer, and brain cancer [ 19 ].…”