The Earth is adorned with a rich and varied array of flora. It has been estimated that the botanical diversity in India contributes significantly, comprising about 11.4% of the total global species diversity. In a paradigm shift towards safer and more diverse alternatives, plants, known for their absence/least side effects, are emerging as readily available substitutes for commercial drugs. Traditional pharmaceuticals, often laden with toxicity, high costs, and adverse reactions, are being challenged by the exploration of plant-derived alternatives. This study centers on the synthesis and characterization of silver nanoparticles (AgNPs) derived from the methanol leaf extract of Santalum album. Silver nitrate and leaf methanol extracts are employed separately as the precursor and capping reducing agent. The characterization of the synthesized silver nanoparticles is conducted through various techniques, including UV-Visible Spectrophotometry (UV), Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and Fourier-transform Infrared Spectroscopy (FT-IR). Antimicrobial assessments involve testing against both gram-positive bacteria (Escherichia coli and Klebsiella pneumoniae) and gram-negative bacteria (Staphylococcus aureus and Bacillus subtilis), as well as two fungal species (Aspergillus niger and Candida albicans) using the agar well diffusion method. Furthermore, the AgNPs from Santalum album methanol leaf extract exhibit in vitro antioxidant activity, assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Their potential in combating cancer is explored through the evaluation of anticancer properties against MCF-7 cell lines (Breast Cancer cell lines) using the MTT assay and the determination of nuclear morphology via AO/PI staining. The findings of this study suggest that silver nanoparticles from Santalum album methanol leaf extract may serve as a promising natural drug for both the treatment and prevention of cancer progression.