Biosynthesis of silver nanoparticles from <i>Catharanthus roseus</i> leaf extract and assessing their antioxidant, antimicrobial, and wound-healing activities

Al-Shmgani, Hanady S.; Mohammed, Wasnaa Hatif; Sulaiman, Ghassan M.; Saadoon, Ali H.

doi:10.1080/21691401.2016.1220950

Cited by 120 publications

(85 citation statements)

References 25 publications

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“…Antimicrobial actions of BSNPs exhibited dose dependency and all clearance zones can be attributed to phenols, flavonoid and alkaloids present in PCFE that capped the BSNPs [41]. Effective clearance zones for bacterial and fungal strains obtained with BSNPs make them versatile nanobactericide and fungicides [42].…”

Section: Discussionmentioning

confidence: 99%

Augmented photocatalytic, antibacterial and antifungal activity of prunosynthetic silver nanoparticles

Jaffri

Ahmad

2017

Artificial Cells, Nanomedicine, and Biotechnology

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The present study has demystified the first and single step prunosynthesis of the spherical silver nanoparticles (AgNPs) from aqueous fruit extract of angiospermic plant, Prunus cerasifera, which has remarkable fusion of reducing cum stabilizing bioactive components (phenols, anthocyanins, carotenoids, flavonoids, organic acids, tannins and vitamins). Highly stable prunosynthetic AgNPs with 2.04 nm average crystallite size were synthesized in dark and in sunlight at optimized condition of temperature, time and P. cerasifera concentration. Synthesized nanoparticles were characterized through UV-Vis, FTIR, XRD, TGA, SEM and GCMS. Photocatalytic activity of prunosynthetic AgNPs was evaluated for methyl red, erichrome black, methyl blue, bromophenol blue and bromocresol green via UV-Vis. Degradation was achieved (<15 minutes) and expressed as pseudo-first-order kinetics. Prunosynthetic AgNPs demonstrated broad spectrum dose-dependent inhibition (in vitro) in comparison to standard antimicrobial drugs against pathogenic strains X. citri, P. syringae, A. niger, A. flavus, A. fumigatus, A. terreus, P. chrysogenum, F. solani and L. theobromae. Photocatalytic degradation results show the nanobioremediation potential of prunosynthetic AgNPs in indemnifying the persistent environmental pollutants. From the inherently higher inhibition rates for biomimetic prunosynthetic AgNPs, it is envisioned that these can be commercialized as future "green" nanobactericide and nanofungicide at industrial scale economically from nontoxic phytoconstituents.

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Section: Discussionmentioning

confidence: 99%

Augmented photocatalytic, antibacterial and antifungal activity of prunosynthetic silver nanoparticles

Jaffri

Ahmad

2017

Artificial Cells, Nanomedicine, and Biotechnology

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“…The presence of such functional groups increased the stability of the synthesized nanoparticles by preventing the clotting and aggregation of the nanoparticles. The similarities between the spectra, before and after synthesis of nanoparticles, with some marginal shifts in peak positions indicated the presence of residuals from the different plant extracts in the sample which acted as a capping agent to the synthesized AgNPs [44,47]. Therefore, it can be concluded that these secondary metabolites are responsible for the effective stabilization and capping of the synthesized nanoparticles.…”

Section: Characterization Of Agnps Using Ftirmentioning

confidence: 90%

The synergistic effect of biosynthesized silver nanoparticles from a combined extract of parsley, corn silk, and gum arabic: in vivo antioxidant, anti-inflammatory and antimicrobial activities

Helmy

El-Shazly

Seleem

et al. 2020

Mater. Res. Express

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Microbial resistance, oxidative stress, and inflammatory conditions are among the leading causes of death worldwide. In the current work, silver nanoparticles (AgNPs) were biosynthesized using the aqueous extracts of parsley, corn silk (CS), gum Arabic (GA) or combination of the three extracts. The formed nanoparticles were characterized using three techniques including transmission electron microscopy (TEM), UV-visible spectrophotometer and Fourier-transform infrared spectroscopy (FTIR). The antioxidant, anti-inflammatory, and antimicrobial activities were tested for the formed nanoparticles, the aqueous extracts of each of the three plants and their combination. Oxidative stress was induced by alloxan which promoted the development of diabetes mellitus in rats. Inflammation was induced by injecting carrageenan in rats' paws. Pathogenic microorganisms causing serious urinary tract infection (UTI) were selected for the antimicrobial assay. All aqueous extracts and the biosynthesized AgNPs showed variable degrees of antioxidant, anti-inflammatory and antimicrobial activities, however, the AgNPs biosynthesized by the combination of the three aqueous extracts was the most effective one. LC/MS was done to identify the compounds present in the crude extracts that may be responsible for the observed biological activities. LC/MS resulted in the identification of 13 compounds. Docking experiments on COX-1 (cyclooxygenase-1) and COX-2 (cyclooxygenase-2) were performed to determine the compounds responsible for the antiinflammatory activity of the extracts. The results showed that silver nanoparticles synthesized by the combination of the three aqueous extracts are considered promising candidates for the development of antioxidant, anti-inflammatory and antimicrobial agents. photochemical [10], electrochemical [7, 11], reverse micelle [12], thermal decomposition [13], radiation [7,14] and microwave-assisted [7] methods. Most of these methods require the use of hazardous chemicals and high energy for the preparation of nanoparticles. Biological synthesis of nanoparticles involves the use of natural materials such as plants, bacteria, fungi [1,5,15]. The use of plants for the synthesis of nanoparticles have an advantage over other biological methods as it does not involve the use of cell culture, and does not need longer incubation time required for the reduction of metal ions [16,17]. Plants are known to contain various secondary metabolites such as alkaloids, terpenoids, flavonoids and tannins which provide suitable reducing and surface agents for the nanoparticle synthesis and stabilization. Biopolymers such as cellulose, chitosan, alginate, dextran and tree gums are another family of natural sources which were used for the reduction and stabilization of nanoparticles [17,18].Recently, pathogenic bacteria and fungi such as Staphylococci spp., Enterococci spp., Klebsiella pneumoniae, and Pseudomonas spp. demonstrated resistance to commercially available antimicrobial agents at an increasing rate and has become a global thr...

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“…The antioxidant activity was determined by using DPPH according to Al-Shmgani et al [31]. Briefly, 1 mL of the samples was mixed with an equal volume of the DPPH solution (60 µM).…”

Section: Hesperidin Antioxidant Activity Measurementmentioning

confidence: 99%

In Vivo and In Vitro Evaluation of the Protective Effects of Hesperidin in Lipopolysaccharide-Induced Inflammation and Cytotoxicity of Cell

et al. 2020

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(1) Background: Plant flavonoids are efficient in preventing and treating various diseases. This study aimed to evaluate the ability of hesperidin, a flavonoid found in citrus fruits, in inhibiting lipopolysaccharide (LPS) induced inflammation, which induced lethal toxicity in vivo, and to evaluate its importance as an antitumor agent in breast cancer. The in vivo experiments revealed the protective effects of hesperidin against the negative LPS effects on the liver and spleen of male mice. (2) Methods: In the liver, the antioxidant activity was measured by estimating the concentration of glutathione (GSH) and catalase (CAT), whereas in spleen, the concentration of cytokines including IL-33 and TNF-α was measured. The in vitro experiments including MTT assay, clonogenity test, and sulforhodamine 101 stain with DAPI (4′, 6-diamidino-2-phenylindole) were used to assess the morphological apoptosis in breast cancer cells. (3) Results: The results of this study revealed a significant increase in the IL-33 and TNF-α cytokine levels in LPS challenged mice along with a considerable elevation in glutathione (GSH); moreover, the catalase (CAT) level was higher compared to that of the control group. Cytotoxicity of the MCF-7 cell line revealed significant differences among the groups treated with different concentrations when compared to the control groups, in a concentration-dependent manner. Hesperidin significantly inhibited the colony formation of MCF7 cells when compared to that of control. Clear changes were observed in the cell shape, including cell shrinkage and chromatin condensation, which were associated with a later apoptotic stage. (4) Conclusion: The results indicate that hesperidin might be a potential candidate in preventing diseases.

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Biosynthesis of silver nanoparticles from Catharanthus roseus leaf extract and assessing their antioxidant, antimicrobial, and wound-healing activities

Cited by 120 publications

References 25 publications

Augmented photocatalytic, antibacterial and antifungal activity of prunosynthetic silver nanoparticles

Augmented photocatalytic, antibacterial and antifungal activity of prunosynthetic silver nanoparticles

The synergistic effect of biosynthesized silver nanoparticles from a combined extract of parsley, corn silk, and gum arabic: in vivo antioxidant, anti-inflammatory and antimicrobial activities

In Vivo and In Vitro Evaluation of the Protective Effects of Hesperidin in Lipopolysaccharide-Induced Inflammation and Cytotoxicity of Cell

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