Biosynthesis of silver nanoparticles formation from Caesalpinia pulcherrima stem metabolites and their broad spectrum biological activities

Moteriya, Pooja; Chanda, Sumitra

doi:10.1016/j.jgeb.2017.12.003

Cited by 55 publications

(22 citation statements)

References 44 publications

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“…It is observed from this figure that, the reducing activity enhanced by increasing the amount of P. major extract or the produced biosynthesized AgNPs and a dose-dependent manner was observed in this study. The similar result also reported by other researchers and also by our research group in synthesized AgNPs using other herbal extracts 26,63 . The phytochemical constituent in the P. major extract, which is effective in the bioreduction of Ag + to Ag 0 , can be appeared by the FRAP assay.…”

Section: Stability and Reproducibility Of Nanoparticlessupporting

confidence: 91%

Central Composite Design for Optimizing the Biosynthesis of Silver Nanoparticles using Plantago major Extract and Investigating Antibacterial, Antifungal and Antioxidant Activity

Nikaeen

Yousefinejad

Rahmdel

et al. 2020

Sci Rep

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central composite design (ccD) was applied to optimize the synthesis condition of silver nanoparticles (Agnps) using the extract of Plantago major (P. major) seeds via a low cost and single-step process. the aqueous seed extract was applied as both reducing element and capping reagent for green production of Agnps. five empirical factors of synthesis including temperature (Temp), pH, volume of P. major extract (V ex), volume of Agno 3 solution (V Ag) and synthesis time were used as independent variables of model and peak intensity of Surface plasmon Resonance (SpR) originated from nps as the dependent variable. the predicted optimal conditions was determined to be: Temp = 55 °C, pH = 9.9,V ex = 1.5 mL, V Ag = 30 mL, time = 60 min. The characterization of the prepared AgNPs at these optimum conditions was conducted by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), transmission electron microscopy (TEM) and X-ray diffraction (XRD) to determine the surface biofunctionalities. Bio-activity of these Agnps against bacteria and fungi were evaluated based on its assay against Micrococcus luteus, Escherichia coli and Penicillium digitatum. furthermore, antioxidant capacity of these nps was checked using the ferric reducing antioxidant power (fRAp) assay. Nanotechnology is an important field of modern research which has been the principal of various technologies and main innovations; and is expected to be the basis of many other outstanding innovations in future. Because of importance and applications, research and development in nanoscience has been grown throughout the world during a short period of time 1-5. One of the major outputs of these studies is the introduction of new kind of materials in the nano-scale, including nonofilms, nanoparticles, nanowires and nanoclusters. Among different kinds of nanomaterials, metal nanoparticles are well-known due to their interesting properties in optical, electrical, magnetic and catalytic aspects 6. Metal nanomaterials prepared from the noble metals, in particular gold or silver, have attracted great interests due to their generated strong Surface Plasmon Resonance (SPR) 7. This fact should be noted that the SPR is a sensitive indicator of nanostructures geometry, which can be applied to follow the systematic and controlled variations in their geometry during preparation. SPR has been also widely used in sensing field which is known as "plasmonics" 8. During the past few decades, the exceptional properties of AgNPs have introduced them as reliable candidate for using in different fields such as biomedical 9 , antimicrobial and anti-infection medical device 10,11 , water

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Section: Stability and Reproducibility Of Nanoparticlessupporting

confidence: 91%

Central Composite Design for Optimizing the Biosynthesis of Silver Nanoparticles using Plantago major Extract and Investigating Antibacterial, Antifungal and Antioxidant Activity

Nikaeen

Yousefinejad

Rahmdel

et al. 2020

Sci Rep

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“…However, several studies proposed that AgNPs mode of action against bacteria could be related to following points (a) interact with the membrane surface during cell wall synthesis (b) suppression during protein synthesis (c) disruption of transcription process (d) perturbation of metabolic pathways where interact of Ag with the thiol groups of respiratory enzymes of bacteria 17,30 . Furthermore, MICs and MBCs of biogenic AgNPs exhibited good bactericidal activity at low concentrations against tested bacterial strains 78 . No available reports for MICs and MBCs for AgNPs prepared by lichens.…”

Section: Identification Of Lichens Secondary Metabolites By (Gc-ms)mentioning

confidence: 93%

Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

Alqahtani

Othman

Mohammed

2020

Sci Rep

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Recently, increase bacterial resistance to antimicrobial compounds issue constitutes a real threat to human health. One of the useful materials for bacterial control is Silver nanoparticles (AgNPs). Researchers tend to use biogenic agents to synthesize stable and safe AgNPs. The principal aim of this study was to investigate the ability of lichen in AgNPs formation and to find out their suppression ability to MDR bacteria as well as their cytotoxic activity. In the current study, lichens (Xanthoria parietina, Flavopunctelia flaventior) were collected from the south of the Kingdom of Saudi Arabia. Lichens methanolic extracts were used for conversion of Ag ions to AgNPs. Prepared biogenic AgNPs were characterized by Ultraviolet–Visible (UV–Vis) Spectroscopy, Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) and Zeta potential and Energy-Dispersive X-ray Spectroscopy (EDS). Lichens Secondary metabolites were determined by Fourier-Transform Infrared Spectroscopy (FTIR) and Gas Chromatography–Mass Spectrometry (GC–MS). The antibacterial activity and synergistic effect of AgNPs were evaluated against pathogenic bacteria, including gram-positive; Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus (VRE), and gram-negative; (Pseudomonas aeruginosa, Escherichia coli) as well as the reference strains (ATCC) using the agar disk diffusion method. Cytotoxic effect of biogenic AgNPs was tested against HCT 116 (Human Colorectal Cancer cell), MDA-MB-231 (Breast cancer cell), and FaDu (Pharynx cancer cell) by MTT test. TEM imaging showed well-dispersed spherical particles of 1–40 nm size as well as zeta size showed 69–145 nm. Furthermore, FTIR and GC–MS identified various lichen chemical molecules. On the other hand, the highest antibacterial activity of AgNPs was noticed against P. aeruginosa, followed by MRSA, VRE, and E. coli. AgNPs influence on gram-negative bacteria was greater than that on gram-positive bacteria and their synergistic effect with some antibiotics was noted against examined microbes. Moreover, higher cytotoxicity for biogenic AgNPs against FaDu and HCT 116 cell line in relation to MDA-MB-231 was noted. Given the current findings, the biogenic AgNPs mediated by lichens had positive antibacterial, synergistic and cytotoxic powers. Therefore, they might be considered as a promising candidate to combat the multi-drug resistance organisms and some cancer cells.

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“…Therefore, 5 mM concentration of AgNO 3 solution was used for further set of experiments. AgNO 3 at 5 mM has been optimised for AgNPs synthesis through Argemone Mexicana, while 1 mM AgNO 3 has also been optimised for AgNPs synthesis through Ocimum tenuiflorum, Solanum tricobatum, Syzygium cumini, Centella asiatica, Caesalpinia pulcherrima and Citrus sinensis [30][31][32]. After 4 h the brown colour solution was centrifuged and the received pellet was analysed under UV-Vis spectrophotometer after resuspending in DI water.…”

Section: Resultsmentioning

confidence: 99%

Unveiling the cytotoxicity of phytosynthesised silver nanoparticles using Tinospora cordifolia leaves against human lung adenocarcinoma A549 cell line

Mittal

Pal

Sharma

et al. 2020

IET nanobiotechnol.

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Biosynthesis of silver nanoparticles (AgNPs) using plant extract is a cheap, easily accessible and natural process in which the phyto-constituents of the plants act as capping, stabilising and reducing agent. The present study explored the biosynthesis of AgNPs using aqueous leaf extract of Tinospora cordifolia and characterised via various techniques such as Fourier transform infrared, scanning electron microscopy, transmission electron microscopy (TEM), energy dispersive X-ray analysis and X-ray diffraction. Here, TEM confirmed the spherical morphology with 25-50 nm size of synthesised AgNPs. Further, anticancer efficiency of AgNPs synthesised using T. cordifolia leaves were evaluated against human lung adenocarcinoma cell line A549 by MTT, trypan blue assay, apoptotic morphological changes using Annexin V-FITC and Propidium iodide (PI), nuclear morphological changes by DAPI (4, 6-diamidino-2-phenylindole dihydrochloride) staining, reactive oxygen species generation and mitochondrial membrane potential determination. Results confirmed the AgNPs synthesised using T. cordifolia leaves are found to be highly toxic against human lung adenocarcinoma cell line A549.

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Biosynthesis of silver nanoparticles formation from Caesalpinia pulcherrima stem metabolites and their broad spectrum biological activities

Cited by 55 publications

References 44 publications

Central Composite Design for Optimizing the Biosynthesis of Silver Nanoparticles using Plantago major Extract and Investigating Antibacterial, Antifungal and Antioxidant Activity

Central Composite Design for Optimizing the Biosynthesis of Silver Nanoparticles using Plantago major Extract and Investigating Antibacterial, Antifungal and Antioxidant Activity

Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

Unveiling the cytotoxicity of phytosynthesised silver nanoparticles using Tinospora cordifolia leaves against human lung adenocarcinoma A549 cell line

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