Biosynthesis of silver nanoparticle using extract of Zea mays (corn flour) and investigation of its cytotoxicity effect and radical scavenging potential

Abstract

Silver nanoparticles (AgNPs) possesses a number of exceptional pharmaceutical properties and applications as compared with other types of metallic nanoparticles. Currently, AgNPs was biosynthesized using an aqueous extract of Zea mays L. (corn flour) powder. Further, the effect of concentration of reagents, extract, temperature and time of synthesis was also studied along with the cytotoxicity and radical scavenging potential. UV-vis spectra of AgNPs gave a surface plasmon resonance at~420 nm. The absorption peak became sharp with the increase in time. AgNPs with monodispersed and aggregated spherical shape was observed by SEM image followed by its confirmation via strong signal in silver region of EDX spectrum. The XRD spectra confirmed its crystallinity and face-centered cubic structure. FT-IR spectra reveal the presence of phytocompounds in the synthesis of AgNPs. Further, the AgNPs exhibited strong cytotoxicity potential against HepG2 cells and its viability declined with an increase in the concentration of AgNP with respect to the control cells. It also demonstrated reasonable radical scavenging potential in terms of DPPH and ABTS scavenging, and reducing power tests. Taken together, these results of the current investigation stated that AgNPs could be beneficial in biomedical applications particularly for treatment of cancer disease along with its applications in pharmaceutical industries for the formulation of new drugs.

<p>Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of <em>Ipomoea batatas</em> (L.) Lam</p>

“…These results might arise from the interference of several functional groups existing in the Ib1 and Ib2 extracts, which might have played a vital part in the capping and stabilization procedure during Ib1-AgNPs and Ib2-AgNPs synthesis. 22,72,80 The Ib1-AgNPs and Ib2-AgNPs showed higher scavenging effect against DPPH-free radicals in comparison to ABTS radical cations, as evident from less IC 50 values (Table 1), which is consistent with previous reports. 22,72,81 However, Ib2-AgNPs demonstrated slightly more potent antioxidant potential than the Ib1-AgNPs in entire four assays (Table 1), which may be related with the combination of additional oxidants on the external surface area of Ib2-AgNPs.…”

“…22,72,80 The Ib1-AgNPs and Ib2-AgNPs showed higher scavenging effect against DPPH-free radicals in comparison to ABTS radical cations, as evident from less IC 50 values (Table 1), which is consistent with previous reports. 22,72,81 However, Ib2-AgNPs demonstrated slightly more potent antioxidant potential than the Ib1-AgNPs in entire four assays (Table 1), which may be related with the combination of additional oxidants on the external surface area of Ib2-AgNPs. 22,43,72 The reducing power of Ib1-AgNPs and Ib2-AgNPs may possibly be due to the presence of functional groups on the external surface of the particles ( Figure 7C).…”

“…NOx is an essential bioregulatory molecule in the immune, nervous and cardiovascular systems. 22,71,72 In the present study, the biosynthesized Ib1-AgNPs and Ib2-AgNPs displayed a concentration-dependent radical scavenging potential ( Figure 7C). The relationships between AgNPs and NOx under anhydrous, anaerobic conditions and at room temperature help acceptance of electron from AgNPs.…”

“…22,72,81 However, Ib2-AgNPs demonstrated slightly more potent antioxidant potential than the Ib1-AgNPs in entire four assays (Table 1), which may be related with the combination of additional oxidants on the external surface area of Ib2-AgNPs. 22,43,72 The reducing power of Ib1-AgNPs and Ib2-AgNPs may possibly be due to the presence of functional groups on the external surface of the particles ( Figure 7C). NOx is an essential bioregulatory molecule in the immune, nervous and cardiovascular systems.…”

“…The current result is similar with a previously reported result. 10,22,72 The cytotoxicity potential of Ib1-AgNPs and Ib2-AgNPs can be credited to the initiation of intracellular oxidative stress. 22,26 According to the earlier studies, the cytotoxicity activity of Ib1-AgNPs and Ib2-AgNPs might be the end result of oxidative stress induced in the cells rather than the toxic effect of Ag + ions.…”