Biosynthesis of Riboflavin, L. Bulgaricus Factor, and Other Growth Factors. Laboratory and Pilot Plant Studies of Biosynthesis by A. Gossypii Cultivated on Grain Stillage Media

Smiley, K. L.; Sobolov, Murray; Austin, F. L.; Rasmussen, R. A.; Smith, Michael B.; Lanen, J. M. Van; Stone, L.; Boruff, C. S.

doi:10.1021/ie50498a033

Cited by 10 publications

(8 citation statements)

References 7 publications

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“…al., personal communication). Crude nitrogen and vitamin sources, like animal steep liquor, corn steep liquor and distillers" solubles have, however, been preferably employed in large-scale riboflavin production (Pfeifer et al, 1950;Smiley et al, 1951;Tanner et al, 1949).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Aguiar

Silva

Domingues

2015

Biotechnology Advances

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The filamentous fungus Ashbya gossypii has been safely and successfully used for more than two decades in the commercial production of riboflavin (vitamin B2). Its industrial relevance combined with its high genetic similarity with Saccharomyces cerevisiae together promoted the accumulation of fundamental knowledge that has been efficiently converted into a significant molecular and in silico toolbox for its genetic engineering. This synergy has enabled a directed and sustained exploitation of A. gossypii as an industrial riboflavin producer. Although there is still room for optimizing riboflavin production, the recent years have seen an abundant advance in the exploration of A. gossypii for other biotechnological applications, such as the production of recombinant proteins, single cell oil and flavour compounds. Here, we will address the biotechnological potential of A. gossypii beyond riboflavin production by presenting (a) a physiological and metabolic perspective over this fungus; (b) the molecular toolbox available for its manipulation; and (c) commercial and emerging biotechnological applications for this industrially important fungus, together with the approaches adopted for its engineering.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Ashbya gossypii beyond industrial riboflavin production: A historical perspective and emerging biotechnological applications

Aguiar

Silva

Domingues

2015

Biotechnology Advances

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“…Consequently, a fermentation process incorporating continuous sterilization may be successful and profitable, whereas the same process carried out with batch sterilization might give product yield so much lower that the operation would be unprofitable. This is true in riboflavin production by fermentation with the yeastlike organism, Ashbya gossypii {14, 19), in which yields from continuously sterilized media are from three to ten times the yields from batch sterilized media. Carnarius (3) has described the savings in labor and materials that are possible with a continuous sterilization installation.…”

Section: 303mentioning

confidence: 99%

Continuous Sterilization of Media in Biochemical Processes

Pfeifer¹,

Vojnovich²

1952

Ind. Eng. Chem.

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“…Maximum shields were 1.8 mg. per ml., somewhat lower than the maximal yields reported for E. ashbyii. In addition to riboflavin, significant quantities of vitamin Bl2, Lactobacillus bulgaricus factor, and other growth factors were produced in A. gossypii fermentations (368). Media containing stillage and animal proteins such as meat stick have been recommended (368, Sp90).…”

Section: Vitaminsmentioning

confidence: 99%

“…Media containing stillage and animal proteins such as meat stick have been recommended (368, Sp90). Best results were obtained when "flash sterilization" techniques were used in the preparation of the media (313,368).…”

Section: Vitaminsmentioning

confidence: 99%