Biosynthesis of respiratory-tract mucins. A comparison of canine epithelial goblet-cell and submucosal-gland secretions

Stahl, Glenn H.; Ellis, Daniel B.

doi:10.1042/bj1360845

Cited by 30 publications

(8 citation statements)

References 11 publications

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“…Goblet cell mucin secretion is shown to be associated with airway inflammation but not to be under any pharmacological controls (Kim et al 1989), which is not the case in glandular secretion. Further, there are some differences between secretions from submucosal glands and those from goblet cells; i.e., goblet cells are implicated in the secretion of a more acidic mucus glycoprotein than from submucosal glands (Stahl and Ellis 1973). These facts may make it difficult to remove the obstructive impairment due to mucus accumulation in the small airways in the treatment of bronchial asthma with goblet cell hyperplasia resulting in sudden death from asthma.…”

Section: Mucus In the Peripheral Airwaymentioning

confidence: 97%

Bronchial Hyperreactivity in the Peripheral Airways.

Sasaki

Sekizawa

Yamaya

1995

Tohoku J. Exp. Med.

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Section: Mucus In the Peripheral Airwaymentioning

confidence: 97%

Bronchial Hyperreactivity in the Peripheral Airways.

Sasaki

Sekizawa

Yamaya

1995

Tohoku J. Exp. Med.

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“…it has been suggested that goblet cells secrete a more acidic mucous glycoprotein than do submucosal glands [34]. Sputum samples collected during an asthma attack have been shown to be more acidic, compared with those not under going an attack and with other patients with respiratory diseases [35].…”

Section: Discussionmentioning

confidence: 99%

Continuity of airway goblet cells and intraluminal mucus in the airways of patients with bronchial asthma

Shimura¹,

Andoh²,

Haraguchi³

et al. 1996

European Respiratory Journal

143

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The aim of this study was to elucidate the mechanism of the formation of the widespread mucous-plugging observed in autopsied lungs from patients with bronchial asthma.We performed morphometric analysis of airways of autopsied lungs from eight patients with bronchial asthma (Group BA), and compared it with those of six chronic bronchitics (Group CB) and four control patients (Control). The following parameters were measured in paraffin sections: volume proportion of bronchial glands to bronchial wall (Gland%); goblet cell granules to total epithelial layer (Goblet%); intraluminal mucus expressed as the mucus occupying ratio (MOR); volume ratio of intraluminal mucus continuous with goblet cells to total intraluminal mucus (Vc/Vtot%); and surface ratio of the contact surface of intraluminal mucus continuous with goblet cells to the total luminal surface (Sc/Stot%).Gland%, Goblet%, and MOR or inflammatory cell numbers in the airway walls both from Group BA and CB were larger than those from the Control group. However, no significant differences were observed between Group BA and CB in Gland%, Goblet%, MOR or inflammatory cell numbers, except for the eosinophil number: i.e. 23±3, 22±3 and 6±2% in Gland%; 22±9, 5±4 and 2±2% in Goblet%; 10±3, 18±3 and 0.3±0.5% in MOR; 199±68, 10±3 and 2±2 cells·mm -2 in eosinophil number of the peripheral airways from Groups BA, CB and Control, respectively. In contrast, marked and significant increases were observed both in Vc/Vtot% and Sc/Stot% in Group BA compared to Groups CB and Control both in central and peripheral airways: i.e. Vc/Vtot% in the peripheral airways was 53±5, 4±3 and 0.8±0.8% from Groups BA, CB and Control, respectively (BA vs CB or BA vs Control, p<0.01 each).These findings suggest that the continuity of goblet cells and intraluminal mucus or lack of full release of mucus, from goblet cells, is peculiar to asthmatic airways, and may contribute to the formation of mucous-plugs.

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“…In addition, the methods we have established for mucus removal from the intestine may be equally applicable to the lung. Previous reports (Sturgess and Reid, 1972;Stahl and Ellis, 1973) have demonstrated the sensitivity of mucus cells in the glands of the respiratory mucosa and submucosa to cholinergic agents. Thus, just as in the intestine, pilocarpine pretreatment (perhaps in combination with aerosol NAC) may effectively eliminate mucus from the alveoli and the airways, thus improving access of vectors to the target cells.…”

Section: Study 3 Effect Of Pilocarpine On Crypt Mucusmentioning

confidence: 98%

Improving Access to Intestinal Stem Cells as a Step Toward Intestinal Gene Transfer

Sandberg¹,

Lau²,

Jacomino³

et al. 1994

Human Gene Therapy

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In previous studies exploring the intestinal epithelium as a potential site for somatic gene therapy, we concluded that the mucus lining the intestine constitutes a significant barrier to any attempts at gene transfer via the lumenal route. The mucus problem is aggravated by the fact that the epithelial stem cells, which are the logical target for gene transfer, are located deep in the intestinal crypts. The goals of the current study were to develop procedures that would improve accessibility to the intestinal stem cells and which would effect in vivo mucus removal without damaging the underlying epithelium. Initial experiments involved evaluation of the use of distension to improve accessibility to the intestinal crypts and the use of the mucolytic agents dithiothreitol (DTT) and N-acetyl-cysteine (NAC) versus a control solution of phosphate-buffered saline (PBS) for mucus removal. Catheters were inserted in each end of 3-cm terminal ileal segments in anesthetized rats. Two milliliters of agent was instilled into the clamped segment for 2 min, removed, and repeated. Lumenal distension resulted in shortened villi with wider intervillus spacing, thereby improving crypt access. Both NAC and DTT washes removed significant mucus between the villi but failed to reach the crypt lumen. To enhance mucus release from the crypt lumen, pilocarpine was selected due to its cholinergic properties and preferential binding to muscarinic receptors on crypt goblet cells. Pilocarpine given intraperitoneally 30 min prior to the mucolytic or PBS wash resulted in significant eradication of mucus down into the crypt lumen. This effect was still evident 3-4 hr later provided the intestine remained undisturbed.

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Biosynthesis of respiratory-tract mucins. A comparison of canine epithelial goblet-cell and submucosal-gland secretions

Cited by 30 publications

References 11 publications

Bronchial Hyperreactivity in the Peripheral Airways.

Bronchial Hyperreactivity in the Peripheral Airways.

Continuity of airway goblet cells and intraluminal mucus in the airways of patients with bronchial asthma

Improving Access to Intestinal Stem Cells as a Step Toward Intestinal Gene Transfer

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