1980
DOI: 10.1042/bj1920507
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Biosynthesis of rat liver pyruvate kinase. Measurement of enzyme lifetime and the rate of synthesis at weaning

Abstract: Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of immunoprecipitates of liver cytosol with anti-(L-type pyruvate kinase) serum revealed proteins of mol.wt. 56 000 and 42 000 in addition to the heavy and light chains. The ratio of the 56 000 mol.wt. to the 42 000 mol.wt. protein increased under dietary conditions that resulted in an increase in the apparent specific activity of hepatic pyruvate kinase. The 42 000 mol.wt. protein was removed from immunoprecipitates if the liver cytosol was partially … Show more

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Cited by 14 publications
(20 citation statements)
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“…After this the rate was restored slightly just before weaning to about 75 of that at birth. Weaning dramatically boosted the synthesis of the L isozyme, which was 44 higher than that at birth and 11 times of that on day 3 which was in accordance with that observed by Hopkirk and Bloxham [20].…”
Section: Apparent Rate Of Synthesis Ojthe Isozymes During Developmentsupporting
confidence: 80%
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“…After this the rate was restored slightly just before weaning to about 75 of that at birth. Weaning dramatically boosted the synthesis of the L isozyme, which was 44 higher than that at birth and 11 times of that on day 3 which was in accordance with that observed by Hopkirk and Bloxham [20].…”
Section: Apparent Rate Of Synthesis Ojthe Isozymes During Developmentsupporting
confidence: 80%
“…Our anti-(L-pyruvate kinase) antibodies also demonstrated at least two distinct parallel arcs upon diffusion against the crude liver extract which was in agreement with the finding of Kohl and Cottam [23]. In the present study this problem was bypassed by subjecting the immunoprecipitate to sodium dodecyl sulphate/polyacrylamide gel electrophoresis followed by slicing and counting the radioactivity resided in the region corresponding to the subunit of the L-type pyruvate kinase according to the procedure of Hopkirk and Bloxham [20]. In all cases the sufficiency of antibody was established by an absence of a precipitin line upon double diffusion of the supernatant after immunoprecipitation against fresh antibodies.…”
Section: Spec$city Of' Antibodiessupporting
confidence: 79%
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