Biosynthesis of Protein Amino Acids in Plant Tissue Culture IV Isotope Competition Experiments using Glucose-U-<sup>14</sup>C and Potential Intermediates

Dougall, Donald K.; Fulton, Michael M.

doi:10.1104/pp.42.7.941

Cited by 30 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After 9 days, INVESTIGATIONS OF many apects of cell physiology and metabolism have been facilitated by the use of liquid suspension cultures of higher plant tissue. Examples include detailed studies of cell division (Stuart and Street, 1969), cell wall synthesis (Rubery and Northcote, 1970), proteinamino acid biosynthesis (Dougall and Fulton, 1967), and acetate metabolism (Fletcher and Beevers, 1970).…”

mentioning

confidence: 99%

Formation and Dissociation of Cell Aggregates in Suspension Cultures of Paul's Scarlet Rose

Wallner

Nevins

1973

American J of Botany

View full text Add to dashboard Cite

In liquid culture stem tissue of Paul's Scarlet rose produces a suspension containing cell aggregates of extremely variable dimensions. There is, however, a definite pattern of change in the degree of cell aggregation over time. During the period of most rapid cell division large aggregates form as the result of a minimal separation of the proliferating cells. As the rate of cell division slows, the average number of cells per aggregate decreases. The dissociation of cell aggregates continues at a uniform rate after cell division has stopped. Cell separation is inhibited at low (0.1 mg/1) auxin (NAA) concentrations and by substitution of sucrose for glucose in the culture medium. Cell separation is delayed (but not greatly inhibited) by kinetin. The presence of casein hydrolysate prevents the formation of the large cell aggregates normally occurring in the early stages of the culture cycle. A variant strain which shows a much higher degree of cell separation has been isolated from stock callus tissue grown on solid medium.

show abstract

mentioning

confidence: 99%

Formation and Dissociation of Cell Aggregates in Suspension Cultures of Paul's Scarlet Rose

Wallner

Nevins

1973

American J of Botany

View full text Add to dashboard Cite

show abstract

“…Imidazoleacetol is not accepted by this enzyme as a substrate. This transaminase activity has also been found in higher plants (5). The dihydroxyacetone phosphate aminotransferase described in this report has similarities to the imidazoleacetol phosphate enzyme; that is, it uses a keto phosphate as the amino acceptor, and cannot use the keto alcohol as a substrate (Fig.…”

mentioning

confidence: 70%

Serinol Phosphate as an Intermediate in Serinol Formation in Sugarcane

Babczinski¹,

Matern²,

Strobel³

1978

Plant Physiol.

View full text Add to dashboard Cite

4To whom request for reprints should be addressed. MATERIALS AND METHODSPlants. Sugarcane (Saccharum officinarum) clones 51NG97, susceptible to H. sacchari, and H50-7209, resistant to this fungus, were obtained from R. Coleman, USDA, Beltsville, Md. The stalks were grown in large plastic pots at 22 ± 5 C under greenhouse conditions. Leaves used in this study were taken from the upper parts of the stalk after they had matured.Enzyme Preparation. Crude enzyme preparations were obtained from leaves that had been deribbed, cut into pieces (2 x 2 mm) with scissors, placed in 0.2 M K-phosphate buffer (4 g of leaf material/15 ml) (pH 8), 1 mm EDTA, 1% PVP and ground in a Sorvall homogenizer at top speed for 1 min. The crude leaf juice was centrifuged at 3,000g for 10 min. A 2-ml aliquot was then transferred to a column of Sephadex G-25 (1.5 x 6 cm) in a plastic syringe whose bottom support end was equipped with a small mesh nylon net. The whole syringe was centrifuged at 1,000g for 1 min and the fluid that passed through the column was taken as the crude enzyme preparation (1.8 mg of protein/ml). Each step in the procedure was performed at 0 to 4 C in precooled vessels. This technique effectively removed many of the harmful phenols and quinones from the preparation.Substrates. The method of Bublitz and Kennedy (3) was used for the preparation of [14C]dihydroxyacetone phosphate after several modifications. The reaction mixture contained 42.2 ,umol of dihydroxyacetone and 5 units of glycerol kinase, (EC 1.7.1.30) which had been desalted immediately before use. At 7-min intervals on four separate occasions, 10 ,umol of ATP(Na salt) and 10 Jumol of MgCl2 were added. The final preparation of [14C]dihydroxyacetone phosphate possessed a specific radioactivity of 0.78 ,uCi/,umol. Serinol was prepared by the method of Szammer (17)

show abstract

“…The most direct relationship of these 3 amino acids is to be found in their sequential biosynthesis. Each is a metabolic derivative of aspartic acid, and their formation can be schematically written as: (8,13). If lysine and threonine act to prevent the synthesis of methionine, then the fact that homoserine can effectively replace methionine in negating the inhibitory effects of lysine-threonine suggests that the site of action of Lysine and threonine were both present in all experiments at concentrations of 100 uM, either alone (0) or with added methionine between 2 and 40 pM (0).…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effects of Metabolically Related Amino Acids on the Growth of a Multicellular Plant

Dunham

Bryan

1969

Plant Physiol.

View full text Add to dashboard Cite

The effects of several amino acids related to the metabolism of aspartic acid on the growth and development of gemmalings of the liverwort Marchantia polymorpha were investigated under axenic conditions. Lysine and theonine synergistically inhibit the growth of these plants and cause a loss of normal pigmentation at concentrations as low as 1 mum. These effects are highly specific for this pair of amino acids, are partially reversible upon removal of the effectors, and can be prevented by low concentrations of methionine or its metabolic precursor, homoserine. Alterations in the growth and development of gemmalings in the presence of natural amino acids are discussed in relation to metabolic regulatory mechanisms which have been well established in microorganisms.

show abstract

Biosynthesis of Protein Amino Acids in Plant Tissue Culture IV Isotope Competition Experiments using Glucose-U-¹⁴C and Potential Intermediates

Cited by 30 publications

References 28 publications

Formation and Dissociation of Cell Aggregates in Suspension Cultures of Paul's Scarlet Rose

Formation and Dissociation of Cell Aggregates in Suspension Cultures of Paul's Scarlet Rose

Serinol Phosphate as an Intermediate in Serinol Formation in Sugarcane

Synergistic Effects of Metabolically Related Amino Acids on the Growth of a Multicellular Plant

Contact Info

Product

Resources

About

Biosynthesis of Protein Amino Acids in Plant Tissue Culture IV Isotope Competition Experiments using Glucose-U-14C and Potential Intermediates

Cited by 30 publications

References 28 publications

Formation and Dissociation of Cell Aggregates in Suspension Cultures of Paul's Scarlet Rose

Formation and Dissociation of Cell Aggregates in Suspension Cultures of Paul's Scarlet Rose

Serinol Phosphate as an Intermediate in Serinol Formation in Sugarcane

Synergistic Effects of Metabolically Related Amino Acids on the Growth of a Multicellular Plant

Contact Info

Product

Resources

About

Biosynthesis of Protein Amino Acids in Plant Tissue Culture IV Isotope Competition Experiments using Glucose-U-¹⁴C and Potential Intermediates