Biosynthesis of polyunsaturated fatty acids by two newly cold-adapted Egyptian marine yeast

Adel, A.; El-Baz, Ashraf F.; Shetaia, Yousseria M.; Sorour, Noha M.

doi:10.1007/s13205-021-03010-4

Cited by 10 publications

(18 citation statements)

References 58 publications

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“…The third important parameter influencing the fatty acid profile was the yeast incubation temperature. In Adel et al [ 56 ], a study conducted at three temperatures of 7 °C, 15 °C, and 26 °C, it was shown that the most unsaturated acids 63.4% are obtained at 15 °C, compared with 57.4% at 7 °C and 43.5% at 26 °C. All our experiments regarding FAME analyses were carried out at the temperature of 25 °C.…”

Section: Resultsmentioning

confidence: 99%

Fungistatic Effect of Phthalide Lactones on Rhodotorula mucilaginosa

et al. 2023

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Currently, there is an increasing number of cases of fungal infections caused by opportunistic strains of the yeast Rhodotorula mucilaginosa, mainly in immunocompromised patients during hospitalization. The excessive use of antibiotics and azole compounds increases the risk of resistance to microorganisms. A new alternative to these drugs may be synthetic phthalide lactones with a structure identical to or similar to the natural ones found in celery plants, which show low toxicity and relatively high fungistatic activity. In the present study, the fungistatic activity of seven phthalide lactones was determined against R. mucilaginosa IHEM 18459. We showed that 3-n-butylidenephthalide, the most potent compound selected in the microdilution test, caused a dose-dependent decrease in dry yeast biomass. Phthalide accumulated in yeast cells and contributed to an increase in reactive oxygen species content. The synergistic effect of fluconazole resulted in a reduction in the azole concentration required for yeast inhibition. We observed changes in the color of the yeast cultures; thus, we conducted experiments to prove that the carotenoid profile was altered. The addition of lactones also triggered a decline in fatty acid methyl esters.

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Section: Resultsmentioning

confidence: 99%

Fungistatic Effect of Phthalide Lactones on Rhodotorula mucilaginosa

et al. 2023

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“…1). The marine environment has always been attractive to researchers, because of its diversity of living organisms that can be exploited to produce various compounds, such as therapeutic metabolites, antibiotics, and nanoparticles (Adel et al 2021;Kumar et al 2021). In this regard, Ulva fasicata (genus Ulva), a genus of green algae (family Ulvaceae), represents one of the natural marine sustainable resources that contain various bioactive compounds, commonly used in nutraceutical and pharmaceutical industries (Namvar et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Biomediated nanosized ZnS using Ulva fasciata and Citrus japonica: A new bio-photocatalyst for textile wastewater treatment

Nady¹,

Abbas²,

Sorour³

2022

Rend. Fis. Acc. Lincei

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The aquatic ecosystems face excessive stress and depletion due to many pollutants impacting water quality. Biosynthesis of nanoparticles (NPs) using the green route represents a cost-effectively and eco-friendly approach, with significant applicability in various fields. In this regard, ZnS-NPs with improved photocatalytic and antimicrobial activities were biosynthesized for the first time from Ulva fasciata and Citrus japonica aqueous extracts. Biosynthesized ZnS-NPs were characterized and compared with chemically synthesized ones using UV–Vis spectroscopy, FTIR, TEM, XRD, and EDAX. TEM micrographs and XRD confirmed the formation of polygonal and spherical-shaped ZnS blend sphalerite nanocrystals with an average diameter between 7 and 31 nm. The optical properties of the produced ZnS-NPs showed higher band-gap energy of 5.63 eV and 4.76 eV for chemically and biosynthesized ZnS-NPs, respectively, as compared to ZnS macromolecules (3.77 eV). Biosynthesized ZnS-NPs showed excellent photocatalytic activity toward textile wastewater and two organic dyes (crystal violet and safranin), with significant degradation efficiency of 82–96.8, 82.5–98.12, and 84–95.9%, respectively. Biosynthesized ZnS-NPs showed high stability up to three subsequent cycles of photodegradation and exhibited promising antimicrobial activity against different Gram-positive and Gram-negative bacteria and Candida albicans. Biosynthesized ZnS-NPs have a less cytotoxic effect (IC50 > 300 μg/mL) than chemically synthesized ZnS-NPs (IC50 88 μg/mL) on human skin fibroblast normal cell lines. The photocatalytic efficiency of the biosynthesized ZnS-NPs has some advantages, such as non-toxic products, cost-effectiveness, and antimicrobial activity, which give them superiority to be used as a safe bio-photocatalyst for water treatment. Graphical abstract

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“…In fed-batch SSF fed with 2.5% substrate, the highest lipid concentration of 26.74 g L −1 was reached at low enzyme loading. In another study conducted by Santek et al [50], they also evaluated the yeast Cutaneotrichosporon oleaginosum (formerly Trichosporon oleaginosus) cultured in a lignocellulosic hydrolysate. On the other hand, Kamal et al [76] utilized hydrolysates from the microalga Arthrospira platensis that were converted into lipids by the oleaginous yeast Cutaneotrichosporon curvatum ATCC 20509 (formerly Apiotrichum curvatum).…”

Section: Discussionmentioning

confidence: 99%

“…Table 1 shows the physicochemical characterization of the residue. SO 4 , 1%%; yeast extract, 0.05%; and glucose, 6.0%; adjusted to pH 6.0 [50]. Previously, the yeast strains were inoculated in 100 mL of SMM for 36 h at 150 rpm and 25 • C (preculture).…”

Section: Industrial Residual Substrate Vinasse (Irv) P5mentioning

confidence: 99%

Lipid Production from Native Oleaginous Yeasts Isolated from Southern Chilean Soil Cultivated in Industrial Vinasse Residues

Díaz-Navarrete,

Marileo,

Madrid

et al. 2023

Microorganisms

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In this research, six strains of oleaginous yeasts native to southern Chile were analyzed for their biotechnological potential in lipid accumulation. For this purpose, the six strains, named PP1, PP4, PR4, PR10, PR27 and PR29, were cultivated in a nitrogen-deficient synthetic mineral medium (SMM). Then, two strains were selected and cultivated in an industrial residual “vinasse”, under different conditions of temperature (°C), pH and carbon/nitrogen (C/N) ratio. Finally, under optimized conditions, the growth kinetics and determination of the lipid profile were evaluated. The results of growth in the SMM indicate that yeasts PP1 and PR27 presented biomass concentrations and lipid accumulation percentages of 2.73 and 4.3 g/L of biomass and 36.6% and 45.3% lipids, respectively. Subsequently, for both strains, when cultured in the residual vinasse under optimized environmental conditions, biomass concentrations of 14.8 ± 1.51 g/L (C/N 80) and 15.83 ± 0.57 g/L (C/N 50) and lipid accumulations of 28% and 30% were obtained for PP1 and PR27, respectively. The composition of the triglycerides (TGs), obtained in the culture of the yeasts in a 2 L reactor, presented 64.25% of saturated fatty acids for strain PR27 and 47.18% for strain PP1. The saturated fatty acid compositions in both strains are mainly constituted of fatty acids, myristic C 14:0, heptadecanoic C 17:0, palmitic C 16:0 and stearic C 18:0, and the monounsaturated fatty acids constituted of oleic acid C 18:1 (cis 9) (28–46%), and in smaller amounts, palmitoleic acid and heptadecenoic acid. This work demonstrates that the native yeast strains PP1 and PR27 are promising strains for the production of microbial oils similar to conventional vegetable oils. The potential applications in the energy or food industries, such as aquaculture, are conceivable.

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Biosynthesis of polyunsaturated fatty acids by two newly cold-adapted Egyptian marine yeast

Cited by 10 publications

References 58 publications

Fungistatic Effect of Phthalide Lactones on Rhodotorula mucilaginosa

Fungistatic Effect of Phthalide Lactones on Rhodotorula mucilaginosa

Biomediated nanosized ZnS using Ulva fasciata and Citrus japonica: A new bio-photocatalyst for textile wastewater treatment

Lipid Production from Native Oleaginous Yeasts Isolated from Southern Chilean Soil Cultivated in Industrial Vinasse Residues

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