Biosynthesis of Polyhydroxyalkanoates (PHA) from Vegetable Oils and Their By-Products by Wild-Type and Recombinant Microbes

Lakshmanan, Manoj; Zainab-L, Idris; Chee, Jiun Yee; Sudesh, Kumar

doi:10.1201/9780429296611-12

Cited by 3 publications

(4 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When neglecting the water content in the substrate of 47.2% (Saad et al, 2021 ), an even higher conversion yield of 0.70 g PHA g dry FPE −1 was achieved. Such a yield is very similar to PHA yields reported for other cultivations with oleaginous (waste) feedstock (Lakshmanan et al, 2020 ; Riedel & Brigham, 2019 , 2020 ).…”

Section: Resultssupporting

confidence: 88%

“…Such valorization is a great opportunity to accelerate a transition towards a circular bioeconomy. In particular, oleaginous residuals represent a very promising feedstock due to the high carbon content, low culture dilutions in fed‐batch processes, high conversion rates to PHA and the possibility to produce short‐chain length ( scl , C‐atoms <6) and medium‐chain length ( mcl , 5 < C‐atoms < 15) PHA or copolymers thereof (Lakshmanan et al, 2020 ; Riedel et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polyhydroxyalkanoate production from animal by‐products: Development of a pneumatic feeding system for solid fat/protein‐emulsions

Gutschmann

Högl

Huang

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

Fat‐containing animal by‐product streams are locally available in large quantities. Depending on their quality, they can be inexpensive substrates for biotechnological processes. To accelerate industrial polyhydroxyalkanoate (PHA) bioplastic production, the development of efficient bioprocesses that are based on animal by‐product streams is a promising approach to reduce overall production costs. However, the solid nature of animal by‐product streams requires a tailor‐made process development. In this study, a fat/protein‐emulsion (FPE), which is a by‐product stream from industrial‐scale pharmaceutical heparin production and of which several hundred tons are available annually, was evaluated for PHA production with Ralstonia eutropha . The FPE was used as the sole source of carbon and nitrogen in shake flask and bioreactor cultivations. A tailored pneumatic feeding system was built for laboratory bioreactors to facilitate fed‐batch cultivations with the solid FPE. The process yielded up to 51 g L −1 cell dry weight containing 71 wt% PHA with a space–time yield of 0.6 g PHA L −1 h −1 without using any carbon or nitrogen sources other than FPE. The presented approach highlights the potential of animal by‐product stream valorization into PHA and contributes to a transition towards a circular bioeconomy.

show abstract

Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoate production from animal by‐products: Development of a pneumatic feeding system for solid fat/protein‐emulsions

Gutschmann

Högl

Huang

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

“…They can be produced by industrial fermentation from various renewable resources and biogenic waste streams (Andreasi Bassi et al, 2021 ; Bhatia et al, 2021 ; Riedel & Brigham, 2019 , 2020 ; Taguchi & Matsumoto, 2021 ; Wang et al, 2021 ). Oleaginous feedstocks are particularly attractive for PHA production because of their high carbon contents, high conversion rates, and lower culture dilution in fed‐batch processes compared with feeding sugar‐based solution (Lakshmanan et al, 2020 ). Specifically, based on stoichiometric estimations, 1 g of linoleic acid could yield 1.38 g of polyhydroxybutyrate (PHB), whereas glucose theoretically yields 0.48 g PHB g Glucose −1 (Akiyama et al, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory‐ and pilot‐scale

Gutschmann

Simões

Schiewe

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

Bioconversion of waste animal fat (WAF) to polyhydroxyalkanoates (PHAs) is an approach to lower the production costs of these plastic alternatives. However, the solid nature of WAF requires a tailor‐made process development. In this study, a double‐jacket feeding system was built to thermally liquefy the WAF to employ a continuous feeding strategy. During laboratory‐scale cultivations with Ralstonia eutropha Re2058/pCB113, 70% more PHA (45 g PHA L −1 ) and a 75% higher space–time yield (0.63 g PHA L −1 h −1 ) were achieved compared to previously reported fermentations with solid WAF. During the development process, growth and PHA formation were monitored in real‐time by in‐line photon density wave spectroscopy. The process robustness was further evaluated during scale‐down fermentations employing an oscillating aeration, which did not alter the PHA yield although cells encountered periods of oxygen limitation. Flow cytometry with propidium iodide staining showed that more than two‐thirds of the cells were viable at the end of the cultivation and viability was even little higher in the scale‐down cultivations. Application of this feeding system at 150‐L pilot‐scale cultivation yielded in 31.5 g PHA L −1 , which is a promising result for the further scale‐up to industrial scale.

show abstract

“…PHA is a family of microbial polyesters that is completely biodegradable and possesses the thermal and mechanical properties of some commonly used conventional plastics. The environmentally friendly PHA is an intracellular product synthesized by Gram-positive, Gram-negative bacteria, and Archaebacteria via the fermentative process on a wide range of substrates such as vegetable oils, sugars, fatty acids, and glycerol 3 , 4 . Though PHA is a desirable material, the high cost of carbon substrate and downstream recovery has become the major restriction for its commercialization.…”

Section: Introductionmentioning

confidence: 99%

Potential of mealworms used in polyhydroxyalkanoate/bioplastic recovery as red hybrid tilapia (Oreochromis sp.) feed ingredient

Zainab-L

Sudesh

2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHAs) are bio-based polymers produced in bacterial cells to replace some petrochemical plastics. It has always been a challenge to commercialise PHA due in part to the costly recovery processes of the PHA granules from the bacterial cells. The biological approach of using mealworms, Tenebrio molitor, for the recovery of PHA from the bacterial cells is a newly established method that is at the scale-up stage. On the other hand, the aquaculture feed industry needs a low-cost mealworm meal as a protein source. We aimed at studying the nutritional value of the mealworms (which are by-products) used for the poly(3-hydroxybutyrate) (PHB) (the most common type of PHA) recovery from the bacterial and examining the effect of the mealworms on the growth performance, and feed utilization efficiency of red hybrid tilapia (Oreochromis sp.). The cells were fed to the mealworms to digest the proteinaceous cellular materials and excrete the PHB granules in the form of fecal pellets. The resulting mealworms were used as fishmeal replacement to formulate five isonitrogenous (35% crude protein) and isolipidic (8% lipid) diets at mealworm meal (MwM) inclusion levels of 0% (MwM0/control diet), 25% (MwM25), 50% (MwM50), 75% (MwM75) or 100% (MwM100). The results showed good nutritive value mealworms [high protein (75%), low-lipid (10%)] and up to 75% MwM inclusion diet was good in supplying satisfactory nutrients and energy to the red hybrid tilapia. This approach is beneficial in a way that minimal cost was involved in recovering kilograms of PHB and the proteins, lipids, and minerals from the bacterial cells do not end up as wastes but in turn, are used as nutrition by the larvae.

show abstract

Biosynthesis of Polyhydroxyalkanoates (PHA) from Vegetable Oils and Their By-Products by Wild-Type and Recombinant Microbes

Cited by 3 publications

References 1 publication

Polyhydroxyalkanoate production from animal by‐products: Development of a pneumatic feeding system for solid fat/protein‐emulsions

Polyhydroxyalkanoate production from animal by‐products: Development of a pneumatic feeding system for solid fat/protein‐emulsions

Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory‐ and pilot‐scale

Potential of mealworms used in polyhydroxyalkanoate/bioplastic recovery as red hybrid tilapia (Oreochromis sp.) feed ingredient

Contact Info

Product

Resources

About