Biosynthesis of polyhydroxyalkanoates from vegetable oil under the co-expression of fadE and phaJ genes in Cupriavidus necator

Polyhydroxyalkanoates Production: A Challenge for the Plastic Industry

“…Another approach to take advantage of the metabolism of this microorganism is the modification of the routes for the intake of the carbon sources, where mutants can metabolize desired substrates. 200,201 The highest reported value of productivity for this strain is 3.1 g/L h using a semicontinuous system with biomass recirculation, which reached 148 g/L DCW with 76 wt % PHB accumulation. 202 Mathematical optimization of the five-stage system proposed by Atlićet al allowed for a PHB productivity of 2.14 g/L h and avoided substrate inhibition.…”

“…The most studied bacterium for the production of PHAs is C. necator, which grows easily on simple sugars and can accumulate polymer content of up to 90% dry weight. , This bacterium uses the so-considered simplest metabolic pathway to produce PHAs, which involves three enzymes whose genes have been identified and expressed in other microbial platforms. Another approach to take advantage of the metabolism of this microorganism is the modification of the routes for the intake of the carbon sources, where mutants can metabolize desired substrates. , The highest reported value of productivity for this strain is 3.1 g/L h using a semicontinuous system with biomass recirculation, which reached 148 g/L DCW with 76 wt % PHB accumulation . Mathematical optimization of the five-stage system proposed by Atlić et al allowed for a PHB productivity of 2.14 g/L h and avoided substrate inhibition. , The first stage of the reactor cascade was modeled according to the principle of the nutrient-balanced continuous biomass production, whereas the second one was a two-substrate-controlled process.…”

Avocado Waste Biorefinery: Towards Sustainable Development

“…Furthermore, different ratios of arabinose/avocado oil to obtain PHAs have been assessed; this is of particular importance since 0.1% (w/v) of arabinose has proven to encourage the wild-type synthase in C. necator H16 to express itself and polymerize longer-side chain monomers such as 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD) when compared to co-polymers made entirely of 3-hydroxybutyrate. This structural modification has a direct effect on the properties of the polymer; in this case, the melting temperature of the co-polymers is dramatically lowered by the inclusion of even trace amounts of 3HO and 3HD [82]. Hence, the culture medium composition should be properly optimized and aimed at producing the bioplastic with the required conditions for the target application.…”