Biosynthesis of poly(3-hydroxybutyrate) (PHB) by Cupriavidus necator from various pretreated molasses as carbon source

Sen, Khok Yong; Hussin, M. Hazwan; Baidurah, Siti

doi:10.1016/j.bcab.2018.11.006

Cited by 73 publications

(26 citation statements)

References 33 publications

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“…Industrial byproducts are complex carbon sources and many times they are constituted of impurities which may impair the microbial growth, such as salts and residual methanol regarding crude glycerol from biodiesel production (Cavalheiro et al, 2009 ) or phenolic compounds and additional contaminants from raw cane juice, when sugarcane molasses is used as a complex carbon source (Sen et al, 2019 ). Sugarcane vinasse is generally a low sugar, nitrogen, and phosphorus byproduct though high levels of organic matter and cations such as potassium, calcium, and magnesium are present in this low pH effluent (Christofoletti et al, 2013 ).…”

Section: Resultsmentioning

confidence: 99%

“…Sucrose hydrolysis generally is required to improve bacteria consumption of simple sugars in molasses, and thus a pre-treatment of sugarcane molasses is necessary to release the hydrolysis products glucose and fructose prior bacterial fermentation. The sucrose hydrolysis can be accomplished via different methods using acid or alkali, enzymes such as invertases, subcritical water and cation exchange resins (Sen et al, 2019 ). The most classical PHA producer Cupriavidus necator (former Ralstonia eutropha ) is unable to assimilate sucrose as carbon source (Arikawa et al, 2017 ) and a pre-treatment of sucrose-containing feedstocks is required for bacterial cultivations in order to obtain PHA biopolymers.…”

Section: Resultsmentioning

confidence: 99%

“…The most classical PHA producer Cupriavidus necator (former Ralstonia eutropha ) is unable to assimilate sucrose as carbon source (Arikawa et al, 2017 ) and a pre-treatment of sucrose-containing feedstocks is required for bacterial cultivations in order to obtain PHA biopolymers. Sen et al ( 2019 ) reported a maximum P(3HB) production of 0.8 g/L and intracellular polymer accumulation of 27% CDW by C. necator from hydrothermal acid pre-treated molasses. In this work, B. glumae MA13 was able to produce PHAs from both sucrose and sugarcane molasses without any pre-treatment resulting in a maximum polymer production of 2.7 g/L and intracellular polymer accumulation of 46.6% CDW, which is a substantial result from a metabolic, economic, and industrial point of view.…”

Section: Resultsmentioning

confidence: 99%

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Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept

Paula

Paula-Elias

Rodrigues

et al. 2021

Front. Bioeng. Biotechnol.

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Polyhydroxyalkanoate (PHA) bioplastic was synthesized by Burkholderia glumae MA13 from carbon sources and industrial byproducts related to sugarcane biorefineries: sucrose, xylose, molasses, vinasse, bagasse hydrolysate, yeast extract, yeast autolysate, and inactivated dry yeast besides different inorganic nitrogen sources. Sugarcane molasses free of pre-treatment was the best carbon source, even compared to pure sucrose, with intracellular polymer accumulation values of 41.1–46.6% cell dry weight. Whereas, xylose and bagasse hydrolysate were poor inducers of microbial growth and polymer synthesis, the addition of 25% (v/v) sugarcane vinasse to the culture media containing molasses was not deleterious and resulted in a statistically similar maximum polymer content of 44.8% and a maximum PHA yield of 0.18 g/g, at 34°C and initial pH of 6.5, which is economic and ecologically interesting to save water required for the industrial processes and especially to offer a fermentative recycling for this final byproduct from bioethanol industry, as an alternative to its inappropriate disposal in water bodies and soil contamination. Ammonium sulfate was better even than tested organic nitrogen sources to trigger the PHA synthesis with polymer content ranging from 29.7 to 44.8%. GC-MS analysis showed a biopolymer constituted mainly of poly(3-hydroxybutyrate) although low fractions of 3-hydroxyvalerate monomer were achieved, which were not higher than 1.5 mol% free of copolymer precursors. B. glumae MA13 has been demonstrated to be adapted to synthesize bioplastics from different sugarcane feedstocks and corroborates to support a biorefinery concept with value-added green chemicals for the sugarcane productive chain with additional ecologic benefits into a sustainable model.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept

Paula

Paula-Elias

Rodrigues

et al. 2021

Front. Bioeng. Biotechnol.

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“…Likewise, production of PHAs from other low‐cost wastes as carbon source has been extensively studied for other bacteria: Pseudomonas hydrogenovora , from cheese whey (25·4 g per 100 g DCW) (Koller et al ); R. eutropha from molasses (27·0 g per 100 g DCW) (Sen et al ), and Thermos thermophilus HB8 , from lactose (35·0 g per 100 g DWC) (Jiang et al ).…”

Section: Resultsmentioning

confidence: 99%

Forest soil bacteria able to produce homo and copolymers of polyhydroxyalkanoates from several pure and waste carbon sources

Clifton-García

González-Reynoso

Robledo‐Ortíz

et al. 2020

Lett Appl Microbiol

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Two bacterial strains able to produce polyhydroxyalkanoates (PHAs) from a wide variety of pure carbon sources (dextrose, xylose, sucrose, lactose and glycerol) were isolated from forest soils and identified as Achromobacter mucicolens and Stenotrophomonas rhizophila. Achromobacter mucicolens also produced poly(3‐hydroxybutyrate) (PHB) from different wastes (cheese whey, molasses, agave bagasse hydrolysate, nejayote and mango waste pulp). Stenotrophomonas rhizophila, produced the copolymer poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHB‐co‐HV) from glycerol (7·7 mol% of HV), and from sucrose with addition of propionic or valeric acid (4·5 and 25 mol% of HV, respectively). The copolymers presented a lower melting point (145, 156 and 127°C) and crystallinity (23, 26 and 16%) than PHB. The maximum biopolymer accumulation (PHB) for each strain growing in pure carbon source was as follows: 31·3 g per 100 g dry cell weight (DCW) for A. mucicolens from xylose; and 13·7 g per 100 g DCW for S. rhizophila from sucrose. Regarding the waste carbon sources, the highest PHB accumulation was obtained from agave bagasse hydrolysate (20·4 g per 100 g DCW) by A. mucicolens. The molecular weights of the biopolymers obtained ranged from 200 to 741 kDa. Significance and Impact of the Study The economic cost of the carbon source for the culture of polyhydroxyalkanoates (PHAs)‐producing microorganisms is one of the main process limitations. Therefore, it is vital to find versatile microorganisms able to grow and to accumulate homo and copolymers of PHAs from low‐cost substrates. In this research, we report two bacterial strains that produce poly(3‐hydroxybutyrate), poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) or both from at least five pure and five waste carbon sources. These results, by such bacterial strains have not been reported, especially the production of copolymer from glycerol without addition of precursors by Stenotrophomonas rhizophila and the production of PHB from xylose and agave bagasse hydrolysate by Achromobacter mucicolens.

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“…Consequently, rather than decompose, these plastics tend to accumulate in landfills, oceans, and the natural environment. Replacement of petroleum-derived plastics with bioplastic such as polyhydroxyalkanoates (PHAs) and poly(lactic acid) (PLA) has attracted much attention due to their beneficial properties, which are similar to conventional polymers and, additionally, they possess other important benefits such as biodegradability, biocompatibility, and non-toxic behaviour [ 9 , 10 ]. These unique properties of biopolymers gave rise to diverse applications in industries ranging from biodegradable packaging materials to biocompatible medical devices and tissue engineering [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

A Review of the Applications and Biodegradation of Polyhydroxyalkanoates and Poly(lactic acid) and Its Composites

et al. 2021

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Overconsumption of plastic goods and improper handling of petroleum-derived plastic waste have brought a plethora of negative impacts to the environment, ecosystem and human health due to its recalcitrance to degradation. These drawbacks become the main driving force behind finding biopolymers with the degradable properties. With the advancement in biopolymer research, polyhydroxyalkanoate (PHA) and poly(lacyic acid) (PLA) and its composites have been alluded to as a potential alternative to replace the petrochemical counterpart. This review highlights the current synthesis process and application of PHAs and PLA and its composites for food packaging materials and coatings. These biopolymers can be further ameliorated to enhance their applicability and are discussed by including the current commercially available packaging products. Factors influencing biodegradation are outlined in the latter part of this review. The main aim of this review article is to organize the scattered available information on various aspects of PHAs and PLA, and its composites for packaging application purposes. It is evident from a literature survey of about 140 recently published papers from the past 15 years that PLA and PHA show excellent physical properties as potential food packaging materials.

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Biosynthesis of poly(3-hydroxybutyrate) (PHB) by Cupriavidus necator from various pretreated molasses as carbon source

Cited by 73 publications

References 33 publications

Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept

Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept

Forest soil bacteria able to produce homo and copolymers of polyhydroxyalkanoates from several pure and waste carbon sources

A Review of the Applications and Biodegradation of Polyhydroxyalkanoates and Poly(lactic acid) and Its Composites

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