Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant Escherichia coli from glucose

Hokamura, Ayaka; Wakida, Izumi; Miyahara, Yuki; Tsuge, Takeharu; Shiratsuchi, Hideki; Tanaka, Kenji; Matsusaki, Hiromi

doi:10.1016/j.jbiosc.2015.01.022

Cited by 18 publications

(7 citation statements)

References 36 publications

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“…[ 113 ] More recently, poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyalkanoate) containing different fractions of MCL‐3HA units of C8, C10, C12, and C14, has been synthesized to achieve desirable physical properties. [ 114 ]…”

Section: Biodegradable Biopolymersmentioning

confidence: 99%

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Sohn

Kim

Baritugo

et al. 2020

Biotechnology Journal

112

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Advances in scientific technology in the early twentieth century have facilitated the development of synthetic plastics that are lightweight, rigid, and can be easily molded into a desirable shape without changing their material properties. Thus, plastics become ubiquitous and indispensable materials that are used in various manufacturing sectors, including clothing, automotive, medical, and electronic industries. However, strong physical durability and chemical stability of synthetic plastics, most of which are produced from fossil fuels, hinder their complete degradation when they are improperly discarded after use. In addition, accumulated plastic wastes without degradation have caused severe environmental problems, such as microplastics pollution and plastic islands. Thus, the usage and production of plastics is not free from environmental pollution or resource depletion. In order to lessen the impact of climate change and reduce plastic pollution, it is necessary to understand and address the current plastic life cycles. In this review, "sustainable biopolymers" are suggested as a promising solution to the current plastic crisis. The desired properties of sustainable biopolymers and bio-based and bio/chemical hybrid technologies for the development of sustainable biopolymers are mainly discussed.

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Section: Biodegradable Biopolymersmentioning

confidence: 99%

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Sohn

Kim

Baritugo

et al. 2020

Biotechnology Journal

112

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“…The detected molecular mass of 124.3 kDa was in the range of that of PHA MCL(-co-SCL) produced by different recombinant strains in previous studies (3,(45)(46)(47), but was lower than PHA MCL commonly produced by the Pseudomonas wild-type strains (2,48). The quantities of PhaC molecules, which catalyze polymerization, have been shown to be related to the number of polymer chains and consequently influence the individual molecular weight of each chain.…”

Section: Discussionmentioning

confidence: 66%

Synthesis Gas (Syngas)-Derived Medium-Chain-Length Polyhydroxyalkanoate Synthesis in Engineered Rhodospirillum rubrum

Heinrich

Raberg

Fricke

et al. 2016

Appl Environ Microbiol

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The purple nonsulfur alphaproteobacterium Rhodospirillum rubrum S1 was genetically engineered to synthesize a heteropolymer of mainly 3-hydroxydecanoic acid and 3-hydroxyoctanoic acid [P(3HD-co-3HO)] from CO-and CO 2 -containing artificial synthesis gas (syngas). For this, genes from Pseudomonas putida KT2440 coding for a 3-hydroxyacyl acyl carrier protein (ACP) thioesterase (phaG), a medium-chain-length (MCL) fatty acid coenzyme A (CoA) ligase (PP_0763), and an MCL polyhydroxyalkanoate (PHA) synthase (phaC1) were cloned and expressed under the control of the CO-inducible promoter P cooF from R. rubrum S1 in a PHA-negative mutant of R. rubrum. P(3HD-co-3HO) was accumulated to up to 7.1% (wt/wt) of the cell dry weight by a recombinant mutant strain utilizing exclusively the provided gaseous feedstock syngas. In addition to an increased synthesis of these medium-chain-length PHAs (PHA MCL ), enhanced gene expression through the P cooF promoter also led to an increased molar fraction of 3HO in the synthesized copolymer compared with the P lac promoter, which regulated expression on the original vector. The recombinant strains were able to partially degrade the polymer, and the deletion of phaZ2, which codes for a PHA depolymerase most likely involved in intracellular PHA degradation, did not reduce mobilization of the accumulated polymer significantly. However, an amino acid exchange in the active site of PhaZ2 led to a slight increase in PHA MCL accumulation. The accumulated polymer was isolated; it exhibited a molecular mass of 124.3 kDa and a melting point of 49.6°C. With the metabolically engineered strains presented in this proof-of-principle study, we demonstrated the synthesis of elastomeric second-generation biopolymers from renewable feedstocks not competing with human nutrition. IMPORTANCEPolyhydroxyalkanoates (PHAs) are natural biodegradable polymers (biopolymers) showing properties similar to those of commonly produced petroleum-based nondegradable polymers. The utilization of cheap substrates for the microbial production of PHAs is crucial to lower production costs. Feedstock not competing with human nutrition is highly favorable. Syngas, a mixture of carbon monoxide, carbon dioxide, and hydrogen, can be obtained by pyrolysis of organic waste and can be utilized for PHA synthesis by several kinds of bacteria. Up to now, the biosynthesis of PHAs from syngas has been limited to short-chain-length PHAs, which results in a stiff and brittle material. In this study, the syngas-utilizing bacterium Rhodospirillum rubrum was genetically modified to synthesize a polymer which consisted of medium-chain-length constituents, resulting in a rubber-like material. This study reports the establishment of a microbial synthesis of these so-called medium-chain-length PHAs from syngas and therefore potentially extends the applications of syngas-derived PHAs. Biodegradable and biocompatible polyhydroxyalkanoates (PHAs) are naturally occurring polyesters, which are synthesized by various bacteria. Depending on their...

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“…However, most of the researches for microbial production of PHAs are investigated on organic acids, fatty acid and plant oil as the substrate, especially by using recombinants of R.eutropha or Escherichia coli with improved the intracellular content and the monomer composition of polyester in recent years [12][13][14][15]. Biosynthesis of copolymer from "unrelated carbon source" like glucose is also studied using engineered strains [16][17][18][19][20][21][22]. There are only several researches reported for the autotrophic production from CO 2 [23].…”

Section: Discussionmentioning

confidence: 99%

Autotrophic Growth of Paracoccus denitrificans in Aerobic Condition and the Accumulation of Biodegradable Plastics from CO<sub>2</sub>

Tanaka¹,

Mori²,

Hirata³

et al. 2016

eer

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The cell growth on H 2 and O 2 as the energy source and CO 2 as the sole carbon source in the autotrophic culture condition was tested for the gram-negative bacteria Paracoccus spp. The aerobic growth in the autotrophic condition was only observed in Paracoccus denitrificans NBRC13301 and P.pantotrophus NBRC102493. Both strains were sensitive to O 2 in particular the growth of P.pantotrophus was completely inhibited at the concentrations above 2% O 2. P.denitrificans grew until 15% O 2 however the optimum O 2 concentration was 5%. The growth characteristic of P.denitrificans was investigated by pH-controlled batch culture with supplying the gas mixture of H 2 , O 2 and CO 2. The specific growth rate was 0.12 h-1 at 5% O 2 , 30°C and pH7.0. The growth was much slower than other hydrogen-oxidizing bacteria however P.denitrificans accumulated biodegradable plastic, polyhydroxybutyrate, PHB with the content of 57.3% w/w in the cell under nitrogen limitation. Under DO limitation, the cell concentration increased to 25 g/L without accumulating PHB if NH 3 solution as the nitrogen source was fed sufficiently.

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Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant Escherichia coli from glucose

Cited by 18 publications

References 36 publications

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Recent Advances in Sustainable Plastic Upcycling and Biopolymers

Synthesis Gas (Syngas)-Derived Medium-Chain-Length Polyhydroxyalkanoate Synthesis in Engineered Rhodospirillum rubrum

Autotrophic Growth of Paracoccus denitrificans in Aerobic Condition and the Accumulation of Biodegradable Plastics from CO<sub>2</sub>

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