Biosynthesis of phosphatidyl ethanolamine in Mycobacterium 607

Nandedkar, Arvind K.N.

doi:10.1016/0006-2944(75)90102-7

Cited by 6 publications

(10 citation statements)

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“…Enzyme activities (nmol/min per mg of protein) Bacterium E. carotovora Escherichia coli F. rhenanum C. aquaticum K. aerogenes No evidence supported the proposal that ethanolamine was incorporated directly into phosphatidylethanolamine via the CDP-ethanolamine pathway in the case of Mycobacterium 607 (Nandedkar, 1974(Nandedkar, , 1975 or any other bacterium. It seems certain that phosphatidylserine is an obligatory precursor of phosphatidylethanolamine in bacteria and that labelling of the phosphatidyl base from [14C]ethanolamine occurs by an indirect route.…”

Section: Discussionmentioning

confidence: 99%

“…Intramolecular localization of radioactivity in phospholipids Although ['4C]ethanolamine clearly gave rise to lipids other than phospholipids in some bacteria, it was possible that it could be utilized directly to provide the base moiety of phosphatidylethanolamine, as suggested by Nandedkar (1974Nandedkar ( , 1975, particularly in the case of E. carotovora. This bacterium metabolized ethanolamine to acetaldehyde, which was excreted and did not appear to be further metabolized (Jones & Turner, 1971;.…”

Section: Bacteriummentioning

confidence: 99%

“…Although bacteria are believed to synthesize phosphatidylethanolamine via phosphatidylserine, by the CDP-diacylglycerol route (Kanfer & Kennedy, 1964), a role for ethanolamine O-phosphateand CDPethanolamine in phospholipid biosynthesis by a species of Mycobacterium has been proposed (Nandedkar, 1974(Nandedkar, , 1975. The possibility that this Materials and Methods Micro-organisms and media E. carotovora (N.C.P.P.B.…”

mentioning

confidence: 99%

“…The possibility that this Materials and Methods Micro-organisms and media E. carotovora (N.C.P.P.B. 1280) was obtained from the National Collection of Plant Pathogenic Bacteria, Ministry ofAgriculture, Fisheries and Food, Harpenden, Herts., U.K. M. smegmatis ('Mycobacterium 607' of Nandedkar, 1974Nandedkar, , 1975 was obtained as N.C.T.C. 7017 (A.T.C.C.…”

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confidence: 99%

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Microbial metabolism of amino alcohols. Biosynthetic utilization of ethanolamine for lipid synthesis by bacteria

Shukla¹,

Turner²

1980

Biochemical Journal

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1. Ten bacteria utilizing [2-14C]ethanol-2-amine as the sole or major source of nitrogen for growth on glycerol + salts medium incorporated radioactivity into a variety of bacterial substances. A high proportion was commonly found in lipid fractions, particularly in the case of Erwinia carotovora. 2. Detailed studies of [14C]ethanolamine incorporation into lipids by five bacteria, including E. carotovora, showed that all detectable lipids were labelled. Even where phosphatidylethanolamine was the major lipid labelled, radioactivity was predominantly in the fatty acid rather than the base moiety. The labelled fatty acids were identified in each case. 3. The addition of acetate to growth media decreased the incorporation of radioactivity from ethanolamine into both fatty acid and phosphatidyl-base fragments of lipids from all the bacteria except Mycobacterium smegmatis. Experiments with [3H]ethanolamine and [14C]acetate confirmed that unlabelled acetate decreased the incorporation of both radioactive isotopes into lipids, except in the case of M. smegmatis. 4. Enzyme studies suggested one of two metabolic routes between ethanolamine and acetyl-CoA for each of four bacteria. A role for ethanolamine O-phosphate was not obligatory for the incorporation of [14C]ethanolamine into phospholipids, but correlated with CoA-independent aldehyde dehydrogenase activity.

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Section: Discussionmentioning

confidence: 99%

Section: Bacteriummentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Microbial metabolism of amino alcohols. Biosynthetic utilization of ethanolamine for lipid synthesis by bacteria

Shukla¹,

Turner²

1980

Biochemical Journal

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“…Ethanolamine kinase: The assay system for ethanolamine kinase was that described by Nandedkar [17], and consisted of 40 mM Tris-HC1, pH 8-6, 10 mM MgC12, 10 mM cysteine, 2 mM (1,2-~H)-ethanolamine (8.8 mCi mmol-1) and 125-150/zg of enzyme protein in a final volume of 0.25 ml. Phosphorylethanolamine was eluted on Dowex-1-CH3COO-x 8 (100-200 mesh) by the method of Weinhold & Reithy [24].…”

Section: Enzyme Assaysmentioning

confidence: 99%

Subcellular localization of enzymes of phospholipid metabolism inCandida albicans

Mago

Khuller

1990

Med Mycol

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The intracellular location of various enzymes involved in the metabolism of phospholipids of Candida albicans was studied. Among the biosynthetic enzymes, phosphatidylserine synthetase was found to be localized in the microsomes; choline kinase and ethanolamine kinase were cytosolic; acyltransferase was localized in the paniculate fraction and glycerol kinase and phosphatidic acid phosphatase were distributed in both the microsomal and cytosolic fractions. Phospholipase A and phospholipase C were abundant in the microsomes and phospholipase C was also detected in the cytosol. Lysophospholipase and glycerophosphocholine diesterase were distributed mainly in the mitochondria. Lipase activity was also detected in this fungus. Based on the enzymes detected in this study we have postulated pathways of phospholipid metabolism in C. albicans.Phospholipids are vital structural and functional entities of biomembranes [6] and their metabolism is related to the formation and functions of membrane components [ 1 ]. Biosynthesis of phospholipids occurs mainly via the Kennedy and CDP-diacylglycerol pathways, in addition to alternate routes such as base exchange, methylation, acylation and deacylation reactions. Phospholipid degradation takes place by three main pathways. The first includes phospholipase A, lysophospholipase and glycerophosphocholine diesterase, the second involves phospholipase C, and the third involves phospholipase D and results in the formation of phosphatidic acid. Phosphatidic acid is used either to synthesize triglycerides, or is degraded by lipase for conversion to glycerol-3-phosphate by glycerol kinase. The presence of these pathways has been established in mammalian systems, and to some extent in fungi [8,12,23], but in Candida albicans the information is restricted to extracellular phospholipases [4,19]. This communication describes the identification and subcellular distribution of enzymes of phospholipid metabolism in C. albicans. METHODS ChemicalsRadiolabelled (3H)-glycerol (sp. act. 2-50 mCi mmol-1), (3H)-ethanolamine (sp. act.

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Biosynthesis of major phospholipids of Microsporum gypseum

Kasinathan

Khuller

1983

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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Biosynthesis of phosphatidyl ethanolamine in Mycobacterium 607

Cited by 6 publications

References 11 publications

Microbial metabolism of amino alcohols. Biosynthetic utilization of ethanolamine for lipid synthesis by bacteria

Microbial metabolism of amino alcohols. Biosynthetic utilization of ethanolamine for lipid synthesis by bacteria

Subcellular localization of enzymes of phospholipid metabolism inCandida albicans

Biosynthesis of major phospholipids of Microsporum gypseum

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