Biosynthesis of oleandomycin by Streptomyces antibioticus: influence of nutritional conditions and development of resistance

Abstract

The influence of different nutritional compounds on oleandomycin biosynthesis by Streptomyces antibioticus was studied, resulting in the design of a chemically defined medium for production of the antibiotic. Of the variety of carbon and nitrogen compounds tested, fructose and aspartic acid (carbon and nitrogen sources, respectively) supported the highest oleandomycin titres. Addition of propionate but not acetate, both precursors of the skeleton of the macrolide lactone ring, stimulated the biosynthesis of the antibiotic. Oleandomycin biosynthesis was repressed by glucose but not by phosphate. S. antibioticus develops oleandomycin resistance shortly before the antibiotic begins to be synthesized, showing a triphasic pattern of resistance: spores and producing mycelium are resistant, while non-producing mycelium is sensitive.

Identification and characterization of phosphoenolpyruvate: fructose phosphotransferase systems in three Streptomyces species

“…Growth responses to various carbon sources were recorded using salts medium 63 (Miller, 1972;Sabater e t al., 1972) containing 1 % (w/v) carbohydrate and 15 mM asparagine (Vilches et al, 1990). Of the carbon sources tested, all three Streptomyces strains were found to utilize fructose and glucose most avidly.…”

“…bacteria, it is important to understand the pathways responsible for sugar catabolism (Martin & Demain, 1980;Hodgson, 1982;Vilches et al, 1990; Delic e t al., 1 992).…”

Investigation of Siderophore production and Antifungal activity against Phytophthora capsici as related to Iron (III) nutrition by Lysobacter antibioticus HS124

“…This indicated that the synthesis of antifungal metabolites depends on the amount and type of medium constituents. In fact, it has been shown that the carbon and other nutrient sources strongly affect antibiotic production in different organisms, and glucose in particular, increased antibiotic production potential in the rich medium (Cruz et al, 1999;Vilches et al, 1990).…”

Antibacterial Activity of Streptomyces Tunisiensis Mg520500 Isolated from Soil Samples

“…So, this medium was used to determine the optimal conditions that enhance antibacterial productivity by Streptomyces tunisiensis SA-13. The key factors that strongly affect microbial metabolites are carbon and nitrogen sources [34]. The results showed that antimicrobial activity was higher in riched medium having sugar and peptone as carbon and nitrogen source respectively.…”