Biosynthesis of Medium Chain Length Polyhydroxyalkanoates (Mcl-Pha) by Activated Sludge: Impact of Phosphorus Limitation on Pha Production and Microbial Diversity

Alaux, Emilie; Couvreur, Marion; Marie, Bastien; Bounouba, Mansour; Hernandez-Raquet, Guillermina

doi:10.2139/ssrn.4028808

Cited by 2 publications

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“…This intermittent substrate feeding strategy generates a competitive advantage for bacteria that store PHA as carbon and electron reservoir inside their cell. A proof‐of‐principle was already established with C8, C9, C12, and C18 fatty acids (Alaux et al, 2022; Chen et al, 2018; Lee et al, 2011; Shen et al, 2015), where it was found that medium‐chain fatty acids form a suitable substrate for the enrichment of mcl‐PHA producers. The mcl‐PHA fractions of the total PHA content ranged from 0.06 to 0.88.…”

Section: Introductionmentioning

confidence: 99%

Production of medium‐chain‐length PHA in octanoate‐fed enrichments dominated by Sphaerotilus sp.

Vermeer

Depaz

Berg

et al. 2022

Biotech & Bioengineering

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Medium-chain-length polyhydroxyalkanoate (mcl-PHA) production by using microbial enrichments is a promising but largely unexplored approach to obtain elastomeric biomaterials from secondary resources. In this study, several enrichment strategies were tested to select a community with a high mcl-PHA storage capacity when feeding octanoate. On the basis of analysis of the metabolic pathways, the hypothesis was formulated that mcl-PHA production is more favorable under oxygen-limited conditions than short-chain-length PHA (scl-PHA). This hypothesis was confirmed by bioreactor experiments showing that oxygen limitation during the PHA accumulation experiments resulted in a higher fraction of mcl-PHA over scl-PHA (i.e., a PHA content

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