Biosynthesis of makisterone A and 20‐hydroxyecdysone from labeled sterols by the honey bee, Apis mellifera

Feldlaufer, Mark F.; Herbert, Elton W.; Svoboda, James A.; Thompson, M. J.

doi:10.1002/arch.940030502

Cited by 43 publications

(26 citation statements)

References 13 publications

(16 reference statements)

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“…Rachinsky et al (1990) and Robinson et al (1991) also reported that both 20E and Makisteron A were detected as major ecdysteroids in honeybee prepupa and adult queen hemolymph by HPLC-RIA. Feldlaufer et al (1986b) demonstrated, however, that [ 14 C]cholesterol injected into pupae was incorporated into 20E, suggesting that some pupa organs convert cholesterol into 20E. In our experiment, Makisterone A was not detected in the culture medium of fat bodies.…”

Section: Discussioncontrasting

confidence: 79%

Ecdysteroid biosynthesis in workers of the European honeybee Apis mellifera L.

Yamazaki

Kiuchi

Takeuchi

et al. 2011

Insect Biochemistry and Molecular Biology

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Section: Discussioncontrasting

confidence: 79%

Ecdysteroid biosynthesis in workers of the European honeybee Apis mellifera L.

Yamazaki

Kiuchi

Takeuchi

et al. 2011

Insect Biochemistry and Molecular Biology

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“…Campesterol (normally converted into 24-methyl-enecholesterol) is related in A. mellifera to a 28-carbon molting hormone: makisterone A (Feldlaufer et al 1985(Feldlaufer et al , 1986aFeldlaufer and Svoboda 1988). Because A. mellifera lacks a dealkylation mechanism and uses such an uncommon ecdysteroid, these studies suggest that a special metabolic pathway exists in this insect, probably also including 24-methylenecholesterol.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Pollen and Nectar of Arbutus unedo as a Food Source for Bombus terrestris (Hymenoptera: Apidae)

Rasmont¹,

Regali²,

Ings³

et al. 2005

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Abstract:The mineral, total amino acid, and sterol compositions of pollen collected by Apis mellifera L. were compared with the pollen of a plant consumed by Bombus terrestris (L.): Arbutus unedo L. This plant provides the predominant food resource for the main autumn generation of B. terrestris in southern France. Honey bees also forage on this plant, although only for nectar. The mineral composition of 30 pollen samples collected by honey bees is close to the presently known requirements of A. mellifera, except for Cu and Mn, which are substantially lower. The total amino acid mean composition of a set of 54 pollen samples fits the basic requirements of honey bees except for valine, isoleucine, and methionine, which are present in lower concentrations in all the samples. For pollen of A. unedo, the amino acid balance is not very different from that of the survey. The main sterolic component in pollen of A. unedo, β-sitosterol, is known to have antifeedant effects on A. mellifera. Honey bees cannot dealkylate C 29 sterols like β-sitosterol or 85-avenasterol to obtain C 27 cholesterol and ecdysteroids. Because these phytosterols as well as cholesterol are nearly absent from pollen of A. unedo, the metabolic capabilities of Apis seem unadapted to this plant. On the contrary, pollen of A. unedo is freely consumed by B. terrestris, which develops huge autumn populations solely on this food. These data indicate that the sterolic metabolisms of B. terrestris and A. mellifera differ, allowing separation in foraging activity.

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“…A report that the adult milkweed bug, Ovlcopeltus fusciutus, can convert radiolabeled cholesterol to both C27 and C28 ecdysteroids [19] should probably be reinvestigated. The honey bee, for instance, has recently been shown to convert radiolabeled campesterol to makisterone A and radiolabeled cholesterol to 20-hydroxyecdysoner although no interconversion of either c 2 8 sterol to C27 ecdysteroid, or C27 sterol to c 2 8 ecdysteroid was observed [20].…”

Section: Discussionmentioning

confidence: 98%

The neutral sterols of Megalotomus quinquespinosus (say) (hemiptera: Alydidae) and identification of makisterone a as the major free ecdysteroid

Feldlaufer

Svoboda

Aldrich

et al. 1986

Arch Insect Biochem Physiol

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Last-stage nymphs of the broad-headed bug, Megalotomus quinquespinosus contain the C28 ecdysteroid makisterone A as their major ecdysteroid. No ecdysone or 20-hydroxyecdysone was detected in whole body extracts analyzed by high performance liquid chromatography and radioimmune assay. Analyses of the neutral sterols of this phytophagous hemipteran revealed that the sterol composition of the nymphs was highly reflective of their dietary sterols. The most abundant nymphal sterols were sitosterol (46.6%), A'-stigmastenol (13.8%) and spinasterol (13.4%). Cholesterol accounted for only 0.2% of the total sterols and indicates that this species is incapable of converting 24-alkyl sterols to cholesterol.

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Biosynthesis of makisterone A and 20‐hydroxyecdysone from labeled sterols by the honey bee, Apis mellifera

Cited by 43 publications

References 13 publications

Ecdysteroid biosynthesis in workers of the European honeybee Apis mellifera L.

Ecdysteroid biosynthesis in workers of the European honeybee Apis mellifera L.

Analysis of Pollen and Nectar of <I>Arbutus unedo</I> as a Food Source for <I>Bombus terrestris</I> (Hymenoptera: Apidae)

The neutral sterols of Megalotomus quinquespinosus (say) (hemiptera: Alydidae) and identification of makisterone a as the major free ecdysteroid

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