Biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus using oxygen vector and optimum impeller tip speed

Lai, Zee Wei; Rahim, Raha Abdul; Ariff, Arbakariya; Mohamad, Rosfarizan

doi:10.1016/j.jbiosc.2012.04.011

Cited by 28 publications

(25 citation statements)

References 26 publications

(34 reference statements)

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“…Reducing the n- the addition of n-hexadecane to 0.5% v/v, which maximized k L a (Lai et al, 2012). Reducing the n- the addition of n-hexadecane to 0.5% v/v, which maximized k L a (Lai et al, 2012).…”

Section: Vector Concentration Affects Ha Productionmentioning

confidence: 99%

“…Compounds such as n-heptane, n-hexadecane, and perfluorodecalin were preferred as they had been successfully applied to either microbial HA production (Lai et al, 2012; or biopolymer production in B. subtilis (Zhang, Feng, Li, Chen, & Xu, 2012). In the preliminary evaluation, each compound was added to the cultivation medium at a concentration of 1% v/v 2 hr after inoculation.…”

Section: Preliminary Evaluation Of Potential Oxygen Vectorsmentioning

confidence: 99%

“…For example, a substantial increase in viscosity (up to 500-fold) is observed as HA titers exceed 5 g/L in microbial cultivations (Widner et al, 2005). However, shearing due to high agitation and aeration rates may reduce the quality (i.e., MW) of HA (Duan, Yang, Zhang, & Tan, 2008;Gao et al, 2003;Lai, Rahim, Ariff, & Mohamad, 2012), and supplementing pure oxygen can be detrimental to microorganisms as exposure to reactive oxygen species such as H 2 O 2 , O 2 −, and OH• can result in DNA instability, and protein and lipid denaturation (Farr & Kogoma, 1991). Oxygen transfer in microbial cultivations can be improved by increasing agitation and aeration rates, and, if necessary, pure oxygen supplementation.…”

mentioning

confidence: 99%

“…The addition of n-dodecane (Liu & Yang, 2009) and n-hexadecane (Lai et al, 2012) to S. zooepidemicus cultivations resulted in improvements to both the HA titer and MW, while perfluorodecalin also increased the HA titer in S. zooepidemicus cultivations relative to a three-stage agitation operation in which the agitation speed was increased in a step-wise manner . The addition of n-dodecane (Liu & Yang, 2009) and n-hexadecane (Lai et al, 2012) to S. zooepidemicus cultivations resulted in improvements to both the HA titer and MW, while perfluorodecalin also increased the HA titer in S. zooepidemicus cultivations relative to a three-stage agitation operation in which the agitation speed was increased in a step-wise manner .…”

mentioning

confidence: 99%

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Application of hydrocarbon and perfluorocarbon oxygen vectors to enhance heterologous production of hyaluronic acid in engineered Bacillus subtilis

Westbrook

Ren

Moo‐Young

et al. 2018

Biotech & Bioengineering

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In microbial cultivations for hyaluronic acid (HA) production, oxygen can be a limiting substrate due to its poor solubility in aqueous medium and the substantial increase in culture viscosity at relatively low HA titers. Shear stress due to the high agitation and aeration rates required to overcome oxygen limitation may reduce the quality (i.e., molecular weight) of HA, and production costs associated with power consumption and supplemental oxygen may be excessive. Here, we report the application of oxygen vectors to the heterologous production of HA in engineered Bacillus subtilis, leading to significantly improved culture performance. We first derived an improved HA-producing strain of B. subtilis through engineering of the promoter driving coexpression of seHas and tuaD, leading to high-level HA production. Out of seven potential oxygen vectors evaluated in a preliminary screening, significant improvements to the HA titer and/or cell density were observed in cultures containing n-heptane, n-hexadecane, perfluoromethyldecalin, and perfluoro-1,3-dimethylcyclohexane. Adjustments to the vector concentration, timing of vector addition, and the agitation rate resulted in further enhancements, with the HA titer reaching up to 4.5 g/L after only 10 hr cultivation. Moreover, our results indicate that certain vectors may alter the functional expression of Class I hyaluronan synthase (HAS) in B. subtilis, and that higher shear rates may drive more carbon flux through the HA biosynthetic pathway without negatively affecting the MW. Our study demonstrates the efficacy of oxygen vectors to enhance heterologous HA production in B. subtilis, and provides valuable insight for future bioprocess development in microbial HA production.

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Section: Vector Concentration Affects Ha Productionmentioning

confidence: 99%

Section: Preliminary Evaluation Of Potential Oxygen Vectorsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Application of hydrocarbon and perfluorocarbon oxygen vectors to enhance heterologous production of hyaluronic acid in engineered Bacillus subtilis

Westbrook

Ren

Moo‐Young

et al. 2018

Biotech & Bioengineering

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“…oxygenation agent, culture medium and continuously metabolising cells). Water-immiscible liquid gas carriers such as saturated hydrocarbons (Lai et al, 2012;Li et al, 2012;da Silva et al, 2006), oils (Dumont et al, 2006;Han et al, 2009), haemoglobin derivatives Lowe et al, 2001) or perfluorinated compounds (Dumont et al, 2006;Menge et al, 2001;Ntwampe et al, 2010;Pilarek and Szewczyk, 2005) characterised by O 2 /CO 2 solubility much higher than that in water, are alternatives to conventional aeration systems applied in bioreactors and other small (i.e. micro-and milliliter-) scale culture systems.…”

Section: Introductionmentioning

confidence: 99%

Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects

Pilarek¹

2014

Chemical and Process Engineering

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Fully synthetic, biochemically inert and water-immiscible liquid perfluorochemicals (PFCs) are recognised as flexible liquid carriers/scavengers of gaseous compounds (respiratory gases mainly, i.e. O 2 and CO 2 ) and increasingly applied in bioprocess engineering. A range of unmatched physicochemical properties of liquid PFCs, i.e. outstanding chemo-and thermostability, extremely low surface tension, simultaneous hydro-and lipophobicity, which result from carbon chain substitution with fluorine atoms (the most electronegative chemical element) and the presence of intramolecular C-F bonds (the strongest single bond known in organic chemistry) have been described in detail. Exceptional propensity to solubility of respiratory gases in liquid perfluorinated compounds has been widely discussed. Advantages and disadvantages of bioprocess applications of liquid PFCs in the form of a pure PFC as well as in an emulsified form have been pointed out. A liquid PFC-mediated mass transfer intensification in various types of microbial, plant cell and animal cell culture systems: from miniaturised microlitre-scale cultures, via biomaterial-based scaffolds containing culture systems, to litre-scale bioreactors, has been reviewed and elaborated on bearing in mind the benefits of bioprocesses.

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Enhancement of antroquinonol production during batch fermentation using pH control coupled with an oxygen vector

et al. 2018

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Biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus using oxygen vector and optimum impeller tip speed

Cited by 28 publications

References 26 publications

Application of hydrocarbon and perfluorocarbon oxygen vectors to enhance heterologous production of hyaluronic acid in engineered Bacillus subtilis

Application of hydrocarbon and perfluorocarbon oxygen vectors to enhance heterologous production of hyaluronic acid in engineered Bacillus subtilis

Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects

Enhancement of antroquinonol production during batch fermentation using pH control coupled with an oxygen vector

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