Biosynthesis of gold nanoparticles assisted by the intracellular protein extract of Pycnoporus sanguineus and its catalysis in degradation of 4-nitroaniline

Abstract

The development of green procedure for the synthesis of gold nanoparticles (AuNPs) has gained great interest in the field of nanotechnology. Biological synthetic routes are considered to be environmentally benign and cost-effective. In the present study, the feasibility of AuNPs’ synthesis via intracellular protein extract (IPE) of Pycnoporus sanguineus was explored. The characteristics of generated particles of formation, crystalline nature, and morphology and dimension were analyzed by UV-vis spectroscopy, X-ray diffraction (XRD) and transmission electron microscopy (TEM), respectively. UV-vis spectra exhibited strong absorption peaks in 520 to 560 nm, indicating the formation of AuNPs. XRD analysis revealed that the formed AuNPs were purely crystalline in nature. TEM observation showed that AuNPs with various shapes including spherical, pseudo-spherical, triangular, truncated triangular, pentagonal, and hexagonal, ranging from several to several hundred nanometers, were synthesized under different conditions. The average size of AuNPs decreased from 61.47 to 29.30 nm as the IPE addition increased from 10 to 80 mL. When the initial gold ion concentration changed from 0.5 to 2.0 mM, the average size rose from 25.88 to 51.99 nm. As in the case of solution pH, the average size was 84.29 nm with solution pH of 2.0, which diminished to 6.07 nm with solution pH of 12.0. Fourier transform infrared (FTIR) analysis implied that the functional groups including hydroxyl, amine, and carboxyl were involved in the reduction of gold ions and stabilization of AuNPs. The catalysis results showed that 0.019 mg of AuNPs with average size of 6.07 nm could catalyze the complete degradation of 12.5 μmol of 4-nitroaniline within 6 min and the degradation rate increased drastically with the addition of AuNPs. All the results suggested that the IPE of P. sanguineus could be potentially applied for the eco-friendly synthesis of AuNPs.Electronic supplementary materialThe online version of this article (doi:10.1186/s11671-015-0856-9) contains supplementary material, which is available to authorized users.

Green biosynthesis of gold nanoparticles by Lilium casa blanca petals and evaluation of catalytic activity

“…Meanwhile, the mixture discoloured from yellow to colourless as shown in photographs. In contrast, the blank control without the addition of AuNPs showed no change in either colour or absorption peaks even after several days, thus implied the required role of AuNPs in this reduction process, which was in accordance with previous reports [20]. Fig.…”

Biointeraction of Erythrocyte Ghost Membranes with Gold Nanoparticles Fluorescents

“…Synthesized AuNPs have a spherical shape with a normal and narrow particle size distribution and a face-centered cubic crystalline structure; the average diameter and crystalline structure obtained are typical for those obtained by the chemical reduction method, where a ruby red solution is obtained [ 42 , 43 ]. The solution color is indicative of the relationship between the SPR and AuNPs size in contact with visible light; depending on the area of the metallic nanoparticle, visible light diffracts within the electromagnetic spectrum range.…”

Encapsulation of Aconitine in Self-Assembled Licorice Protein Nanoparticles Reduces the Toxicity In Vivo

“…When exposed to biological medium or multiple composition dispersions like herbal decoction, protein corona formed on the nanoparticles (NPs) diversely alters the biological fate and pharmaceutical efficacy of particles [ 10 ]. The intracellular protein extracts of a fungal strain Pycnoporus sanguineus was used as reducing and stabilizing agents to synthesize AuNPs with various shapes and dimensions, which present good catalyzing ability on the degradation of 4-nitroaniline [ 11 ].…”