Biosynthesis of gibberellic acid from milk permeate in repeated batch operation by a mutant Fusarium moniliforme cells immobilized on loofa sponge

Meleigy, Samir A.; Khalaf, Mahmoud

doi:10.1016/j.biortech.2008.06.024

Cited by 72 publications

(52 citation statements)

References 17 publications

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“…For the subsequent cycles, the total FOS concentration obtained at the end of each 24-h cycle was lower, with similar values for cycles 4-8. A similar decreasing trend in the Wnal production level was also observed during the repeated batch fermentation for gibberellic acid production by immobilized cells of Fusarium moniliforme [13].…”

Section: Cells Immobilizationsupporting

confidence: 69%

“…Repeated batch cultivation is a well-known method for enhancing the productivity of microbial cultures because it skips the turnaround time and the lag phase, thus increasing the process productivity [17,18]. This method has been a very useful approach to increase the yields of several processes, such as the production of gibberellic acid [13], hyaluronic acid [18], biohydrogen [19], and lipase [20], and may also be a promising mode for FOS fermentation.…”

Section: Introductionmentioning

confidence: 98%

“…Moreover, immobilized cell systems have an additional advantage in that they promote an increase in the fermentor cell density that consequently contributes to increased productivity [10][11][12]. Therefore, such immobilized cell systems have been used in industrial-scale fermentation processes to obtain a number of diVerent products, such as organic acids, edulcorant, beer, among others [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus

Mussatto

Rodrigues

Teixeira

2009

J Ind Microbiol Biotechnol

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The fungus Aspergillus japonicus ATCC 20236 was immobilized in vegetal fiber and used in repeated batch fermentations of sucrose (200 g/l) for the production of beta-fructofuranosidases (FFase). The assays were performed during eight consecutive cycles that were completed in a total period of 216 h. After each 24-h cycle of fermentation (except for the first cycle, which lasted 48 h), the fermented broth was replaced by fresh medium, and the FFase activity was determined in the replaced medium. The average value of FFase activity was a constant 40.6 U/ml at the end of the initial seven cycles, but had decreased by 22% at the end of the eighth cycle. Concurrent with these high and constant FFase values, the hydrolyzing activity of this enzyme increased during the cycles, while the transfructosylating activity decreased. As a consequence, the maximum production of fructooligosaccharides of 134.60 g/l observed in the initial 30 h of fermentation (first cycle) had gradually decreased by the end of the subsequent cycles, reaching approximately 23% of this value during cycles 4-8. Based on these results, we conclude that the present immobilization system has a great potential for application in a semi-continuous process for the production of FFase, but further studies are necessary to maintain the FFase transfructosylation activity at high levels during the overall process.

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Section: Cells Immobilizationsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus

Mussatto

Rodrigues

Teixeira

2009

J Ind Microbiol Biotechnol

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“…After that period, FOS production (and consequently the Y P/S and Q P results) decreased more sharply, reaching similar values at the two last cycles (86-96 h), lower than 15 g/l. This can be due to an accumulation of dead cells, along the 96 h of process, caused by the biomass reuse [34]. Another possible explanation is the accumulation of secondary products resulting from cellular metabolism, such as ethanol or acetic acid (not quantified in this work), which could negatively influence the subsequent fermentation cycles.…”

Section: Repeated Batch Fermentation Assaysmentioning

confidence: 87%

Production of fructooligosaccharides and β-fructofuranosidase by batch and repeated batch fermentation with immobilized cells of Penicillium expansum

Mussatto

Prata

Rodrigues

et al. 2012

Eur Food Res Technol

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The production of fructooligosaccharides (FOS) and b-fructofuranosidase (FFase) by immobilized cells of Penicillium expansum was evaluated. In an initial stage, different low-cost materials including synthetic fiber, polyurethane foam, stainless steel sponge, loofah sponge, and cork oak were tested as carrier for the fungus immobilization. Additionally, the influence of the inoculum age (1 or 3 weeks) on cells immobilization, FOS and FFase production was also verified. Synthetic fiber and polyurethane foam were the best materials for P. expansum immobilization (2.21 and 1.98 g/g carrier, respectively) and FOS production (120.3 and 104.8 g/l), and gave also high results of FFase activity (23.01 and 32.42 U/ml). Then, the production of FOS and FFase by repeated batch fermentation with P. expansum immobilized on synthetic fiber was studied, aiming to improve the batch fermentation results. The results obtained in this stage were very promising with FOS yields of 87, 72, and 44 %, in the 3 initial cycles (60 h), respectively; the FFase activity was constant throughout the process (6 cycles, 96 h). Repeated batch fermentation with immobilized cells of P. expansum was found as being a technology with great potential for FOS and FFase production on industrial scale.

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“…However, Lale and Gadre (2010) also reported GA production at 20 h by a mutant of G. fujikuroi. Maximum GA yields are reported to be between days 4 and 8 of fermentation for F. moniliforme (Kumar and Lonsane, 1990;Meleigy and Khalaf, 2009;Rangaswamy, 2012;Omojasola and Benu, 2016) and day 6 and 12 for A. niger (Bilkay et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Gibberellic Acid Production by Fusarium moniliforme and Aspergillus niger Using Submerged Fermentation of Banana Peel

Omojasola

Adejoro

2018

Not Sci Biol

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The present study aimed to produce gibberellic acid through fermentation using banana (Musa sapientum) peel waste as substrate. Banana peel, a domestic and industrial waste, constitutes a potential source of cheap fermentable substrate for the production of other value-added products. Fusarium moniliforme ATCC 10052 and Aspergillus niger CBS 513.88 were used as fermenting organisms. The substrate was dried, ground and its proximate composition determined. The powdered substrate was added to a modified CzapekDox broth (a semisynthetic medium), with Carboxyl methylcellulose (CMC) as control. The fermentation conditions were: pH 5.5; inoculum size 1% (5 × 10 5 spores/mL F. moniliforme) (2 × 10 6 spores/mL A. niger); substrate concentration 2 g; temperature 25 ± 2 o C; fermentation time 7 days. The fermentation was optimized by varying pH, inoculum size, substrate concentration and fermentation time. The extracted GA was subjected to infra-red spectroscopy using FT-IR. The parameters which gave the highest GA yields were thereafter combined in a single fermentation. The results of proximate analysis of banana peel substrate revealed 8.65% moisture, 9.54% protein, 5.40% lipids, 11.45% ash, 22.34% crude fibre, and 42.62% carbohydrate. The GA yields of 13.55 g/L and 12.44 g/L were produced from the banana peel substrate and 3.62 and 2.61 g/L from the CMC control by F. moniliforme and A. niger respectively. Under optimized conditions, F. moniliforme produced 17.48 g/L GA, while A. niger produced 13.50 g/L. Extracted GA was similar to standard GA sample and the present results support the potential use of banana peel for fermentative GA production.

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Biosynthesis of gibberellic acid from milk permeate in repeated batch operation by a mutant Fusarium moniliforme cells immobilized on loofa sponge

Cited by 72 publications

References 17 publications

β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus

β-Fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus

Production of fructooligosaccharides and β-fructofuranosidase by batch and repeated batch fermentation with immobilized cells of Penicillium expansum

Gibberellic Acid Production by Fusarium moniliforme and Aspergillus niger Using Submerged Fermentation of Banana Peel

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