Biosynthesis of eriodictyol in citrus waster by endowing P450BM3 activity of naringenin hydroxylation

Zhang, Xingyi; Feng, Yinghui; Hua, Yuanzhe; Zhang, Chuanxi; Fang, Bohuan; Long, Xiang; Pan, Yue; Gao, Bei; Zhang, John Z. H.; Li, Lijun; Ni, Hui; Zhang, Lujia

doi:10.1007/s00253-023-12867-9

Cited by 1 publication

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(2 S )-Eriodyctiol is a flavonoid belonging to the flavanones subclass that is widespread in citrus fruits, vegetables, and medicinally important plants, and with important antioxidant and anticancer properties ( 24 ). Eriodyctiol is an hydroxylated product of (2 S )-naringenin and therefore its production is of interest in the production of high-value molecules from agricultural waste.…”

Section: Resultsmentioning

confidence: 99%

DetSpace: a web server for engineering detectable pathways for bio-based chemical production

Martín Lázaro,

Marín Bautista,

Carbonell

2024

Nucleic Acids Research

View full text Add to dashboard Cite

Tackling climate change challenges requires replacing current chemical industrial processes through the rational and sustainable use of biodiversity resources. To that end, production routes to key bio-based chemicals for the bioeconomy have been identified. However, their production still remains inefficient in terms of titers, rates, and yields; because of the hurdles found when scaling up. In order to make production more efficient, strategies like automated screening and dynamic pathway regulation through biosensors have been applied as part of strain optimization. However, to date, no systematic way exists to design a genetic circuit that is responsive to concentrations of a given target compound. Here, the DetSpace web server provides a set of integrated tools that allows a user to select and design a biological circuit that performs the sensing of a molecule of interest by its enzymatic conversion to a detectable molecule through a transcription factor. In that way, the DetSpace web server allows synthetic biologists to easily design biosensing routes for the dynamic regulation of metabolic pathways in applications ranging from genetic circuits design, screening, production, and bioremediation of bio-based chemicals, to diagnostics and drug delivery.

show abstract

Section: Resultsmentioning

confidence: 99%