Biosynthesis of cytidine diphosphate diglyceride by human platelets

Call, Frank L.; Williams, William J.

doi:10.1172/jci106248

Cited by 13 publications

(8 citation statements)

References 49 publications

(28 reference statements)

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“…On the one hand, PI-specific phospholipase C is inhibited by calcium removal (19,27), leading to less PI breakdown. On the other, active resynthesis is stimulated because the platelet enzymes involved in this process are magnesium-and/or manganese-dependent and are in fact inhibited by calcium (28,(40)(41)(42). Resynthesis of PI implies that platelet stimulation leads to operation of the classical PI-cycle (43) in its entirety.…”

Section: Resultsmentioning

confidence: 99%

Phospholipid Metabolism in Stimulated Human Platelets

Broekman¹,

Ward²,

Marcus³

1980

J. Clin. Invest.

344

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Section: Resultsmentioning

confidence: 99%

Phospholipid Metabolism in Stimulated Human Platelets

Broekman¹,

Ward²,

Marcus³

1980

J. Clin. Invest.

344

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“…Recently, in fact, the sequences leading to CDPDG (16) and MPI (17) have been shown in human platelets. Further branching of this pathway could account for the 'P labeling of PG and DPG (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…More recently it has been found that the addition of succinate stimulates incorporation of 'Pi into phosphoinositide in vitro (15), thus establishing a link between oxidative phosphorylation and the phosphorylation of this lipid. Further, it has been shown lately that human platelet homogenates can convert PA to CDPDG (16) and the latter to MPI (17).…”

Section: Introductionmentioning

confidence: 99%

Quantification of human platelet inositides and the influence of ionic environment on their incorporation of orthophosphate-32P

Cohen¹,

Broekman²,

Verkley³

et al. 1971

J. Clin. Invest.

112

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A B S T R A C T Platelets are a rich source for the study of inositol lipids in man. The substitution of an EDTAKCl solution for the water component of the Bligh and Dyer procedure permitted quantitative extraction of polyphosphoinositides. The latter, with monophosphoinositide, were found to comprise, on a molar basis, 6.7% of total platelet phospholipids. Study of the incorporation of orthophosphate-'P into platelet phospholipids was further simplified by separating eight 'P-labeled lipids, including the inositides, with a single chromatographic development on formaldehyde-treated paper. Particular attention was paid to the influence of ionic environment on the pattern and degree of labeling.In 300 mOsm media major phospholipids other than the inositides were not labeled. Small amounts of label appeared in certain trace phospholipids, notably phosphatidic acid. In 150 mOsm media, labeling of inositides was moderately increased, that of trace phospholipids enormously so. The increased labeling was not solely due to thrombocytolysis since (a) platelet disruption by sonication or freeze-thawing abolished 'P incorporation into phospholipids and (b) in timed studies, restoration of osmolarity to 300 mOsm by addition of hypertonic sorbitol blunted the enhancement effect of previous 150 mOsm exposure. Lowering K and compensatorily increasing Na concentration of 300 mOsm media also stimulated aP labeling of inositides and, to a lesser extent, the trace phospholipids. However, the pattern and degree of stimulation were not as strikingly altered as in the osmolarity studies.

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“…Human platelets were prepared and washed as previously described (9). Platelets, leukocytes, and erythrocytes were counted by routine techniques (13,14).…”

Section: Separation Of Blood Cells and Preparation Of Homogenatesmentioning

confidence: 99%

“…Previous work in this laboratory has demonstrated that human platelets can synthesize cytidine diphosphate diglyceride (CDP diglyceride) from cytidine triphosphate and phosphatidic acid (9). When myoinositol was present during this reaction, CDP diglyceride accumulation decreased, and myoinositol-14C was incorporated into a chloroform-soluble product.…”

Section: Introductionmentioning

confidence: 99%