Biosynthesis of copolyesters in Alcaligenes eutrophus H16 from carbon-13 labeled acetate and propionate

Doi, Yoshiharu; Kunioka, Masao; Nakamura, Yoshiyuki; Soga, Kazuo

doi:10.1021/ma00178a006

Cited by 160 publications

(81 citation statements)

References 5 publications

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“…P(3HB) 17 and P(3HB-co-3MP)s [10][11][12][13]15 were synthesized by using Ralstonia eutropha H16. Films for the enzymatic degradation were compression-molded at 195 C for 5 min under a pressure of 5 MPa, using Toyoseiki Mini Test Press-10, and then quenched to 30 C for crystallization of at least 4 weeks.…”

Section: Materials and Preparation Of Samplesmentioning

confidence: 99%

Enzymatic Hydrolysis of Thioester Linkages in Bacterial Poly(3-hydroxybutyrate-co-3-mercaptopropionate)s by Poly(3-hydroxybutyrate) Depolymerase Isolated from Ralstonia pickettii T1

Zhu

Tanaka

Feng

et al. 2005

Polym J

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KEY WORDSPoly(3-hydroxybutyrate-co-3-mercaptopropionate) / Enzymatic Degradation / Thioester / [DOI 10.1295/polymj.37.711] One of major approaches to modify the bacterial poly(3-hydroxybutyrate) (PHB) while reserving its biodegradability has involved the copolymerization of its monomer with some hydroxyalkanoates (HA) via bacterial fermentation, 1 comprising 3-, 4-, 5-and 6-hydroxyalkanoates with various length of the mainchain or various substituents at different positions, such as 3-hydroxypropionate (3HP), 2-5 3-hydroxyvalerate (3HV) 6,7 and so forth. Recently, Steinbüchel's group [8][9][10][11][12][13][14] has succeeded to isolate a novel class of biopolymers from the poly-(hydroxyalkanoate) (PHA)-accumulating bacterium, comprising one kind of completely different backbone constituents, that is, thioesters, such as poly(3-mercaptopropionate), poly(3-mercaptobutyrate), poly(3-mercaptovalerate), poly(3-hydroxybutyrate-co-3-mercaptopropionate) [P(3HB-co-3MP)] and poly(3-hydroxybutyrate-co-3-mercaptobutyrate).Physical properties, such as solubility, crystallinity and thermal stability, of the biodegradable polymers are modified with the introduction of thioester linkages. 8,15 However, few research have been devoted to the enzymatic hydrolysis of thioester linkages in P(3HB-co-3MP) except for the work most recently published. 16 It is found that the PHA depolymerase isolated from Schlegelella thermodepolymerans is specific for the oxoester linkages and that thioester linkages can not be cleaved by the enzyme. PHA depolymerases are known to be quite specific to the chemical structure and physical state of polyesters. It seems necessary to investigate further the enzymatic hydrolyzability of the thioester linkages with considering the possible substrate specificity of depolymerase. PHA depolymerase isolated from Ralstonia pickettii T1 (PhaZRpi) has been well purified and characterized, and thus is one ideal enzyme to study the degradation of P(3HB-co-3MP)s.In this communication, the enzymatic degradability of thioester linkages in the comonomer compositionally well-fractionated P(3HB-co-3MP)s by PHA depolymerase PhaZRpi is evidenced by analyzing degradation products. It is the first observation of the degradation of thioester linkages by PHA depolymerase. Moreover, in comparison with P(3HB-co-3HP)s, the comonomer compositional dependence of the enzymatic degradability will be established. EXPERIMENTAL Materials and Preparation of SamplesP(3HB) 17 and P(3HB-co-3MP)s 10-13,15 were synthesized by using Ralstonia eutropha H16. Films for the enzymatic degradation were compression-molded at 195 C for 5 min under a pressure of 5 MPa, using Toyoseiki Mini Test Press-10, and then quenched to 30 C for crystallization of at least 4 weeks. The enzyme was isolated from Ralstonia pickettii T1. 19 Analytical ProceduresWide-angle X-ray diffraction (WAXD) measurements were carried out on a Rigaku RINT-2000

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Section: Materials and Preparation Of Samplesmentioning

confidence: 99%

Enzymatic Hydrolysis of Thioester Linkages in Bacterial Poly(3-hydroxybutyrate-co-3-mercaptopropionate)s by Poly(3-hydroxybutyrate) Depolymerase Isolated from Ralstonia pickettii T1

Zhu

Tanaka

Feng

et al. 2005

Polym J

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“…The main reasons, which have until now hampered greater diffusion of PHA, are the high cost of the selection of the microorganism and of the substrate (Anderson and Dawes, 1990). Indeed, most processes for PHA production are based on pure cultures of particular microorganisms (e.g., Ralstonia eutropha) grown on well-defined nutrient-deficient synthetic media (Doi et al, 1987;Lee, 1996). There is therefore a potential for widening market for PHAs, provided that their cost decreases.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable polymers from organic acids by using activated sludge enriched by aerobic periodic feeding

Dionisi

Majone²,

Papa³

et al. 2004

Biotech & Bioengineering

174

100

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This article describes a new process for the production of biopolymers (polyhydroxyalkanoates, PHAs) based on the aerobic enrichment of activated sludge to obtain mixed cultures able to store PHAs at high rates and yields. Enrichment was obtained through the selective pressure established by feeding the carbon source in a periodic mode (feast and famine regime) in a sequencing batch reactor. A concentrated mixture of acetic, lactic, and propionic acids (overall concentration of 8.5 gCOD L(-1)) was fed every 2 h at 1 day(-1) overall dilution rate. Even at such high organic load (8.5 gCOD L(-1) day(-1)), the selective pressure due to periodic feeding was effective in obtaining a biomass with a storage ability much higher than activated sludges. The immediate biomass response to substrate excess (as determined thorough short-term batch tests) was characterized by a storage rate and yield of 649 mgPHA (as COD) g biomass (as COD)(-1) h(-1) and 0.45 mgPHA (as COD) mg removed substrates (as COD(-1)), respectively. When the substrate excess was present for more than 2 h (long-term batch tests), the storage rate and yield decreased, whereas growth rate and yield significantly increased due to biomass adaptation. A maximum polymer fraction in the biomass was therefore obtained at about 50% (on COD basis). As for the PHA composition, the copolymer poly(beta-hydroxybutyrate/beta-hydroxyvalerate) with 31% of hydroxyvalerate monomer was produced from the substrate mixture. Comparison of the tests with individual and mixed substrates seemed to indicate that, on removing the substrate mixture for copolymer production, propionic acid was fully utilized to produce propionylCoA, whereas the acetylCoA was fully provided by acetic and lactic acid.

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“…The use of nonliving bacteria for biosorption process has the obvious advantages of being inexpensive and easy to handle (17). In addition, purple bacteria have recently become very promising tools for purifying waste waters and gases (18)(19)(20)(21), and hydrogen bacteria have been used for the production of biodegradable polyester (22)(23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

Biosorption of Heavy Metal Ions onRhodobacter sphaeroidesandAlcaligenes eutrophusH16

Seki

Suzuki

Mitsueda

1998

Journal of Colloid and Interface Science

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Biosynthesis of copolyesters in Alcaligenes eutrophus H16 from carbon-13 labeled acetate and propionate

Cited by 160 publications

References 5 publications

Enzymatic Hydrolysis of Thioester Linkages in Bacterial Poly(3-hydroxybutyrate-co-3-mercaptopropionate)s by Poly(3-hydroxybutyrate) Depolymerase Isolated from Ralstonia pickettii T1

Enzymatic Hydrolysis of Thioester Linkages in Bacterial Poly(3-hydroxybutyrate-co-3-mercaptopropionate)s by Poly(3-hydroxybutyrate) Depolymerase Isolated from Ralstonia pickettii T1

Biodegradable polymers from organic acids by using activated sludge enriched by aerobic periodic feeding

Biosorption of Heavy Metal Ions onRhodobacter sphaeroidesandAlcaligenes eutrophusH16

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