Biosynthesis of C¹⁴-Specifically Labeled Cellulose by Acetobacter xylinum. I. From D-Glucose-1-C¹⁴ with and without Ethanol¹

Synthesis of cellulose by Acetobacter xylinum. 2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose

“…While the possibility cannot be excluded that carbon from an unknown endogenous source participated in cellulose synthesis, it is clear that the major carbon source was the exogenous glucose. Synthesis of radioactive cellulose from [14C]glucose has been described previously in the cotton boll (Greathouse, 1953) and in growing cultures of A. xylinum (Minor, Greathouse, Shirk, Schwartz & Harris, 1954) and A. acetigenum (Bourne & Weigl, 1954).…”

Metabolic13C-Labeling of An Antitumor (1→3)-β-D-Glucan, SSG, fromSclerotinia sclerotiorumIFO 9395

“…Secondly, in order to understand the migration of the 13C-nucleus in glucose, 13C-NMR studies of the mycelium were done. Figures 7 A and 7B are spectra of mycelium cultured in the presence of [1-13C] or [2][3][4][5][6][7][8][9][10][11][12][13] CJglucose. In both spectra, most signals corresponded to remaining glucose (Fig.…”

“…For cellulose and starch, several investigations of their biosynthesis using 13C_ and 14C-Iabeled glucose have been reported. 11 17) As to (1-+ 3)-f3-o-glucan, only the biosynthesis pathway of curdlan, a linear (1-+ 3)-f3-o-g1ucan, has been reported. 18 ) In this paper, we report the glucose use of the mycelium to produce SSG by using two different culture conditions, in which production of SSG is high and low, assessed by using 13C-nuclear magnetic resonance spectroscopy.…”

Acetic Acid Bacteria

“…'Vith glucose-l-C14 as the sole carbon source, most of the activity (70 per cent) was found in position 1 of the constituent glucose units of the synthe sised cellulose, the rest in C-3 and C-4 (173). 'Vith glucose-l-C14 as the sole carbon source, most of the activity (70 per cent) was found in position 1 of the constituent glucose units of the synthe sised cellulose, the rest in C-3 and C-4 (173).…”

“…Cleavage of the glucose mole cule into two Ca pieces can be inferred, since 3-C and 4-C also become labelled, but the E-M-P pathway is excluded since the 6-C does not become labelled (173). In the presence of ethanol, there is an increased yield of cellulose from glucose (173) or mannitol (174), but neither ethanol nor acetate is significantly incorpo rated into cellulose (175). In the presence of ethanol, there is an increased yield of cellulose from glucose (173) or mannitol (174), but neither ethanol nor acetate is significantly incorpo rated into cellulose (175).…”