Biosynthesis of biotin-vitamers by family enterobacteriaceae.

Ohsugi, Masahiro; Imanishi, Yasuko; Teraoka, Tomomi; Nishimura, Kumiko; Nakao, Shoko

doi:10.3177/jnsv.36.447

Cited by 4 publications

(6 citation statements)

References 22 publications

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“…So, for the closest B7 precursor, dethiobiotin, this conjecture is supported by their uptake competition observed in E. coli (Prakash and Eisenberg, 1974). The reported excretion of B7 vitamers by prototrophic strains of enterobacteria (Ohsugi et al, 1990) supports the hypothesis about their potential role in cross-feeding in microbial communities.…”

Section: Resultssupporting

confidence: 69%

Micronutrient Requirements and Sharing Capabilities of the Human Gut Microbiome

et al. 2019

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The human gut microbiome harbors a diverse array of metabolic pathways contributing to its development and homeostasis via a complex web of diet-dependent metabolic interactions within the microbial community and host. Genomics-based reconstruction and predictive modeling of these interactions would provide a framework for diagnostics and treatment of dysbiosis-related syndromes via rational selection of therapeutic prebiotics and dietary nutrients. Of particular interest are micronutrients, such as B-group vitamins, precursors of indispensable metabolic cofactors, that are produced de novo by some gut bacteria (prototrophs) but must be provided exogenously in the diet for many other bacterial species (auxotrophs) as well as for the mammalian host. Cross-feeding of B vitamins between prototrophic and auxotrophic species is expected to strongly contribute to the homeostasis of microbial communities in the distal gut given the efficient absorption of dietary vitamins in the upper gastrointestinal tract. To confidently estimate the balance of microbiome micronutrient biosynthetic capabilities and requirements using available genomic data, we have performed a subsystems-based reconstruction of biogenesis, salvage and uptake for eight B vitamins (B1, B2, B3, B5, B6, B7, B9, and B12) and queuosine (essential factor in tRNA modification) over a reference set of 2,228 bacterial genomes representing 690 cultured species of the human gastrointestinal microbiota. This allowed us to classify the studied organisms with respect to their pathway variants and infer their prototrophic vs. auxotrophic phenotypes. In addition to canonical vitamin pathways, several conserved partial pathways were identified pointing to alternative routes of syntrophic metabolism and expanding a microbial vitamin “menu” by several pathway intermediates (vitamers) such as thiazole, quinolinate, dethiobiotin, pantoate. A cross-species comparison was applied to assess the extent of conservation of vitamin phenotypes at distinct taxonomic levels (from strains to families). The obtained reference collection combined with 16S rRNA gene-based phylogenetic profiles was used to deduce phenotype profiles of the human gut microbiota across in two large cohorts. This analysis provided the first estimate of B-vitamin requirements, production and sharing capabilities in the human gut microbiome establishing predictive phenotype profiling as a new approach to classification of microbiome samples. Future expansion of our reference genomic collection of metabolic phenotypes will allow further improvement in coverage and accuracy of predictive phenotype profiling of the human microbiome.

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Section: Resultssupporting

confidence: 69%

Micronutrient Requirements and Sharing Capabilities of the Human Gut Microbiome

et al. 2019

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“…Dietary reference intakes for Japanese (2015) specified by the Minister of Health, Labour and Welfare set the adequate intake (AI) of biotin at 4 μg/day for infants in the first 6 months of life, based on the average biotin intake of breast‐fed infants . AI was extrapolated to 10 μg/day for infants aged 6–11 months, 20 μg/day for 1–5‐year‐old children, and gradually increased to 50 μg/day for adults, although a small amount of biotin is synthesized by intestinal bacteria and absorbed . Recently in Japan, biotin deficiency has been reported in infants fed special formulas for medical reasons, including those with milk allergy and congenital metabolic disease, because these formulas contain little biotin .…”

mentioning

confidence: 99%

“…2 AI was extrapolated to 10 μg/day for infants aged 6-11 months, 20 μg/day for 1-5-year-old children, and gradually increased to 50 μg/day for adults, 2 although a small amount of biotin is synthesized by intestinal bacteria and absorbed. 3 Recently in Japan, biotin deficiency has been reported in infants fed special formulas for medical reasons, including those with milk allergy and congenital metabolic disease, [4][5][6][7][8][9][10][11][12][13] because these formulas contain little biotin. 14 Biotin deficiency can lead to reduced methylcrotonyl-CoA carboxylase activity, which causes an increase in urinary 3-hydroxyisovaleric acid (3HIA).…”

mentioning

confidence: 99%

Serum biotin in Japanese children: Enzyme‐linked immunosorbent assay measurement

Wakabayashi

Kodama

Ogawa

et al. 2016

Pediatrics International

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Background: Biotin deficiency has been reported in Japanese infants fed special formulas for medical reasons, including those with milk allergy and congenital metabolic diseases, because these formulas contain little biotin. Serum biotin measurement is useful for diagnosing biotin deficiency. We applied a simple and rapid method to analyze serum biotin, and established normal ranges for children and adults. Methods: Serum biotin in 188 healthy Japanese children aged 0-4 years and in 25 healthy adults was analyzed using a Biotin ELISA Kit (immundiagnostik). The effects of various conditions on the measurement of serum biotin were also examined.Results: Median biotin in children aged 0-4 years was 10.4 ng/dL (IQR, 7.9-13.4 ng/dL), and that in adults was 12.9 ng/dL (IQR, 10.8-15.8 ng/dL). Normal range was 4.7-22.0 ng/dL in children and 8.4-20.5 ng/dL in adults (calculated using twosided 95%CI). Measurements obtained with this method were not affected by frozen storage, freeze-thaw, or hemolysis, indicating that serum biotin can be analyzed accurately under these conditions, with a possible application to plasma samples. Conclusions: Serum biotin was significantly lower in children than in adults, with the normal range being 4.7-22.0 ng/dL in children and 8.4-20.5 ng/dL in adults. This simple and accurate enzyme-linked immunosorbent assay method is useful for diagnosing biotin deficiency.

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“…The precursors of biotin are well-studied. Glutaric acid as well as pimelic acid are reported as biotin biosynthesis precursors [110,111,112,113,114].…”

Section: Precursors and Enhancers Of Biotin Productionmentioning

confidence: 99%

“…Pimelic acid is known to be an important precursor of the intermediate dethiobiotin in biotin biosynthesis. Hence, the addition of pimelic acid resulted in significant increase of the formation of biotin-vitamers for Erwinia aroideae IFO 3830 and Klebsiella pneumoniae IFO 3318 [114]. Moreover, when pimelic acid was not added to the media, biotin-vitamers were not synthesized according to the research of Iwahara et al [115].…”

Section: Precursors and Enhancers Of Biotin Productionmentioning

confidence: 99%

Seleção de leveduras potencialmente produtoras de vitaminas do complexo B e estudo do processo fermentativo visando aplicação em alimentação animal

Suzuki¹

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Scientists have been researching not only on vitamin properties but also its production, longing for facilitating human's life, as global demand on micronutrients boost, either to supply diet habits deficiency, or owing to expansion of application in industry. Microorganisms are capable of producing vitamins as metabolites, which are essential nutrients, working as cofactors or coenzymes in the energy production. So, microbial production has been researched for years. Few microorganisms may produce B-group vitamins, amongst them, Bacillus subtilis and Eremothecium ashbyii, whose vitamin is considered GRAS by U.S. Food and Drug Administration (FDA). Fermentative production has been replacing synthetic vitamin synthesis, leading to a better product, higher productivity and, most importantly, minimizing environmental impact. However, many factors affect each vitamin production since each one has its own physical and chemical characteristics. Thus, it is true that it is essential to overcome many drawbacks faced by the vitamin industry; most significantly, a process that is able to produce high amounts of vitamin per liter in a cheap substrate media so as to be an efficient cost-effective and competitive fermentation process to produce vitamin. Here, an overview of the microbial B-vitamin production is shown, seeing that it has been replacing the chemical production in most cases, because of the need for a clean, environmental-friendly process.

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Biosynthesis of biotin-vitamers by family enterobacteriaceae.

Cited by 4 publications

References 22 publications

Micronutrient Requirements and Sharing Capabilities of the Human Gut Microbiome

Micronutrient Requirements and Sharing Capabilities of the Human Gut Microbiome

Serum biotin in Japanese children: Enzyme‐linked immunosorbent assay measurement

Seleção de leveduras potencialmente produtoras de vitaminas do complexo B e estudo do processo fermentativo visando aplicação em alimentação animal

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