Biosynthesis of 2H-labeled Photochrome Trans-membrane Protein Bacteriorhodopsin by Halobacterium Halobacterium Halobium
Abstract:The semi-preparative biosynthesis of photochrome trans-membrane protein bacteriorhodopsin (output 810 mg), labeled with deuterium on functionally important amino acid residues [2,3,4,5,6-2 H5]phenylalanine, [3,5-2 H2]tyrosine, and [2,4,5,6,7-2 H5]tryptophan was carried out with using a photo-organotrophic halobacterium Halobacterium halobium. The protein was isolated from purple membranes by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.