Biosynthesis of 11-deoxycorticosteroids by teleost ovaries and discussion of their possible role in oocyte maturation and ovulation

Colombo, Lorenzo; Bern, Howard A.; Pieprzyk, Julie; Johnson, Donald W.

doi:10.1016/0016-6480(73)90168-8

Cited by 69 publications

(18 citation statements)

References 29 publications

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“…Steroidal material which coeluted with -deoxycortisol on TLC was isolated from spotted seatrout ovaries. Interestingly, increased synthesis of 11-deoxycortisol has been reported in several other marine perciform fishes upon gonadotropin stimulation (Colombo et al 1973(Colombo et al , 1978. Previous studies have failed to establish a definite relationship between the production of 1 7 ca, 2 0/-P and the onset of FOM in several perciform species (Scott and Canario 1987).…”

Section: Discussionmentioning

confidence: 95%

“…It was suggested in a recent review that 17a,203-P is the natural MIS in most, if not all, teleosts (Scott and Canario 1987). However, convincing evidence for a physiological role for 17o,200-P in teleosts belonging to several orders, in particular some perciform species, is lacking (Colombo et al 1973(Colombo et al , 1978Thomas et al 1987). Recently,17ca,20f, has been identified in incubations of Atlantic croaker (Micropogonias undulatus) oocytes during FOM (Trant et al 1986).…”

Section: Introductionmentioning

confidence: 99%

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Evidence that 17α,20β,21-trihydroxy-4-pregnen-3-one is a maturation-inducing steroid in spotted seatrout

Thomas

Trant

1989

Fish Physiol Biochem

111

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Ovarian steroidogenesis during final oocyte maturation (FOM) in the spotted seatrout (Cynoscion nebulosus) was investigated by incubating ovarian fragments with tritiated pregnenolone, followed by chromatographic separation of the radioactive products. The major tritiated steroid produced during FOM comigrated with 17α,20β,21-trihydroxy-4-pregnen-3-one (20β-dihydro-11-deoxycortisol, 20β-S) on HPLC and TLC. Only minor amounts of radioactive material coeluted with 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-P), 11-deoxycorticosterone (DOC), estradiol-17β and testosterone standards in the HPLC system. Additional chromatography by TLC confirmed the presence of radioactive estradiol-17β and testosterone but not 17α,20β-P and DOC.All the ovarian steroids producedin vitro during FOM were assayed for their ability to induce germinal vesicle breakdown (GVBD) of spotted seatrout oocytes. Twenty grams of ovarian tissue were incubated with human chorionic gonadotropin and exogenous pregnenolone. The steroidal products were purified by HPLC and TLC. Most of the maturation-inducing activity was confined to steroidal material which comigrated in these systems with 20β-S. This material was active at a concentration of 1 ng steroid/ml medium in the GVBD assay. Smaller amounts of material which coeluted with 11-deoxycortisol, DOC, 17α,20β-P and several minor unidentified fractions induced GVBD at concentrations of 10 ng steroid(s)/ml.The structure-activity relationships of authentic steroids in inducing GVBD of spotted seatrout oocytes was investigated. Hydroxylation at the 17α, 20β or 21 positions increased potency to induce GVBD. Steroids with multiple hydroxyl groups at the 17α and 20β positions (17α, 20β-P) and at the 17α, 20β, and 21 positions (20β-S) had maximum biological activity in the GVBD bioassay. The results suggest that 20β-S is a major maturation-inducing steroid in spotted seatrout.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Evidence that 17α,20β,21-trihydroxy-4-pregnen-3-one is a maturation-inducing steroid in spotted seatrout

Thomas

Trant

1989

Fish Physiol Biochem

111

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“…Although it was presumed that such steroids were of interrenal origin, because salmon gonadotrophin was more effective in inducing GVBD in co-culture of ovarian and interrenal tissue than in ovarian tissue alone (Goswami et al, 1974), studies on other species (Microgadus proximus (Gadidae), Leptocottus armatus (Cottidae), D icentrarchus labrax (Serranidae), Diplodus annularis (Sparidae), G illichthys mirabilis (Gobiidae), Gobius jozo (Gobiidae) and Solea impar (Soleidae)) indicated that steroids such as ll-deoxycorticosterone and ll-deoxycortisol (see Fig. 4 for steroid structures) could also be synthesized from exogenous radiolabelled precursors by ovarian tissue (Colombo et al, 1973(Colombo et al, , 1978. The progestogen 17,20fl-dihydroxy-4-pregnen-3-one (17,20tiP) was first identified in plasma of Pacific salmon (Oncorhynchus nerka, Salmonidae) by Idler et al (1960), and later studies showed it to be the most effective steroid in inducing GVBD in pike (Esox lucius, (Jalabert, 1976;Goetz and Bergman, 1978;Nagahama et al, 1983).…”

Section: Androgensmentioning

confidence: 99%

?Classical? and ?non-classical? reproductive steroids in fish

Kime

1993

Rev Fish Biol Fisheries

293

183

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“…Cortisol production by ovary has been reported in human (Yong et al 2000), frog (Gobbetti & Zerani 1993) and sturgeon (Webb et al 2002). Colombo et al (1973) reported that ovarian corticosteroids might act as local hormones mediating pituitary gonadotrophin-induced oocyte …”

Section: Discussionmentioning

confidence: 99%

Effects of gonadotrophin in vivo and 2-hydroxyoestradiol-17β in vitro on follicular steroid hormone profile associated with oocyte maturation in the catfish Heteropneustes fossilis

Mishra¹,

Joy²

2006

Journal of Endocrinology

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An HPLC method was used to tentatively identify progesterone (P 4 ) and its metabolites (17-hydroxyprogesterone (17-P 4 ) and 17,20 -dihydroxy-4-pregnen-3-one (17,20 -P)), corticosteroids (cortisol and corticosterone) and testosterone in ovary/follicular preparations of the catfish Heteropneustes fossilis associated with in vivo or in vitro oocyte maturation/ovulation. A single i.p. injection of human chorionic gonadotrophin (100 IU/fish, sampled at 0, 8 and 16 h) induced oocyte maturation and ovulation, which coincided with significant and progressive increases in 17,20 -P, and P 4 and 17-P 4 , the precursors of the former. Both cortisol and corticosterone also increased significantly. Conversely, testosterone decreased significantly and progressively over time. Under in vitro conditions, incubation of post-vitellogenic (intact) follicles or follicular envelope (layer) with 2-hydroxyoestradiol (2-OHE 2 , 5 µM for 0, 6 and 24 h) elicited a sharp significant increase in 17,20 -P, the increase being higher in the follicular envelope incubate. P 4 and 17-P 4 also registered significant increases over the time with the peak values at 24 h. Cortisol and corticosterone increased significantly in the intact follicle, but not in the follicular envelope incubate. Testosterone decreased significantly in the intact follicle, but increased significantly (24 h) in the follicular envelope incubate. Coincident with these changes, the percentage of germinal vesicle breakdown (GVBD) increased over the time in the intact follicle incubate (48·9% at 6 h and 79·8% at 24 h). Denuded oocytes on incubation with 2-OHE 2 (5 µM) did not produce any significant change in the percentage of GVBD or in the steroid profile. While corticosterone and 17,20 -P were undetected, P 4 , 17-P 4 , cortisol and testosterone were detected in low amounts. The results show that the 2-OHE 2 -induced GVBD response seems to be mediated through the production of 17,20 -P and corticosteroids. It is suggested that hydroxyoestrogens seem to be a component in the gonadotrophin cascade of regulation of oocyte maturation/ovulation in the catfish.

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Biosynthesis of 11-deoxycorticosteroids by teleost ovaries and discussion of their possible role in oocyte maturation and ovulation

Cited by 69 publications

References 29 publications

Evidence that 17α,20β,21-trihydroxy-4-pregnen-3-one is a maturation-inducing steroid in spotted seatrout

Evidence that 17α,20β,21-trihydroxy-4-pregnen-3-one is a maturation-inducing steroid in spotted seatrout

?Classical? and ?non-classical? reproductive steroids in fish

Effects of gonadotrophin in vivo and 2-hydroxyoestradiol-17β in vitro on follicular steroid hormone profile associated with oocyte maturation in the catfish Heteropneustes fossilis

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