Biosynthesis “debugged”: Novel bioproduction strategies

Guterl, Jan‐Karl; Sieber, Volker

doi:10.1002/elsc.201100231

Cited by 53 publications

(31 citation statements)

References 112 publications

(182 reference statements)

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“…Electrosynthesis of methanol, ethanol, butanol, acetate, fumerate, etc., using enzymes cascades should be focused. Similarly, the conversion of waste glycerol, a major intermediate from biodiesel industry [310], to dihydroxyacetone phosphate, synthesis of polyhydroxyalkanoates using volatile acid straems also has higher commercial viability.…”

Section: Enzymes For Electrosynthesismentioning

confidence: 99%

“…Recent report on the methanol production from CO 2 [265], has shown the possibility of using CO 2 as precursor for the synthesis of useful chemicals and fuels at cathode in e-BES. Various novel routes for the product synthesis through enzymatic/chemo-enzymatic processes were reported extensively [310]. Adapting these enzymatic processes at e-BES cathode will have an added advantage of simultaneous power generation and co-factor regeneration.…”

Section: Enzymes For Electrosynthesismentioning

confidence: 99%

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Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Satyawali¹

2013

J Microbial Biochem Technol

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Recent interest in the field of biocommodities production through bioelectrochemical systems has generated interest in the enzyme catalyzed redox reactions. Enzyme catalyzed electrodes are well established as sensors and power generators. However, a paradigm shift in recent science towards the production of useful chemicals has changed the face of biofuel cells, keeping the fuels or chemicals production in the upfront. This review article comprehensively presents the progress in the field of enzyme-electrodes for the production of electricity, fuels and chemicals with an aim to represent a practical outline for understanding the use of single or multiple redox enzymes as electrocatalysts for their electron transfer onto electrodes. It also provides the state-of-the-art information regarding the different existing processes to fabricate enzyme electrodes. Successfully-achieved electroenzymatic anodic and cathodic reactions are further discussed, together with their potential applications. Particular focus was made on the novel single/multiple enzyme systems towards product synthesis and other applications. Finally, techno-economic and environmental elements for industrial processing with enzyme catalyzed bioelectrochemical system (e-BES) are anticipated, in order to provide useful strategies for further development of this technology.

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Section: Enzymes For Electrosynthesismentioning

confidence: 99%

Section: Enzymes For Electrosynthesismentioning

confidence: 99%

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Satyawali¹

2013

J Microbial Biochem Technol

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“…Recently cell-free biosystems for biomanufacturing (CFB2) have been suggested to produce low-value biocommodities as an alternative to whole-cell fermentation [9,[16][17][18][19]. In these cell-free biosystems, a number of enzymes and/or coenzymes are put together in one reactor or even separated by a selective cellular or synthetic membrane for implementing desired biotransformation (Fig.…”

Section: Cell-free Biosystems For Biomanufacturingmentioning

confidence: 99%

Cell-free Biosystems in the Production of Electricity and Bioenergy

Zhu

Tam²,

Zhang

2013

Fundamentals and Application of New Bioproduction Systems

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: Increasing needs of green energy and concerns of climate change are motivating intensive R&D efforts toward the low-cost production of electricity and bioenergy, such as hydrogen, alcohols, and jet fuel, from renewable sugars. Cell-free biosystems for biomanufacturing (CFB2) have been suggested as an emerging platform to replace mainstream microbial fermentation for the cost-effective production of some biocommodities. As compared to whole-cell factories, cell-free biosystems comprised of synthetic enzymatic pathways have numerous advantages, such as high product yield, fast reaction rate, broad reaction condition, easy process control and regulation, tolerance of toxic compound/product, and an unmatched capability of performing unnatural reactions. However, issues pertaining to high costs and low stabilities of enzymes and cofactors as well as compromised optimal conditions for different source enzymes need to be solved before cell-free biosystems are scaled up for biomanufacturing. Here, we review the current status of cell-free technology, update recent advances, and focus on its applications in the production of electricity and bioenergy.

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“…Biotechnological approaches for conversion of biomass into some of these molecules are well established by fermentation [1], [2]. However, these microbial processes show several limitations such as reduced product yield due to by-product formation, maintenance of the cells metabolism and, more importantly, low productivity and product titer due to the toxicity of the products formed [3]. One solution to these problems, which has recently found strong interest, is the elimination of the cell as production vehicle and the application of synthetic enzymatic cascades instead [4], [5], [6], [7], [8].…”

Section: Introductionmentioning

confidence: 99%

“…The enzymes used in the artificial cascade tolerate organic solvents to higher levels than cell based systems, and remain active up to 4%v/v isobutanol. At this concentration, microbial hosts are unable to survive or maintain active metabolism [1], [2], [3], [10]. In contrast to fermentative approaches, the artificial cell-free process is based on thermostable enzymes and hence is optimized to be functional at higher temperatures, leading to faster conversion and a higher product yield.…”

Section: Introductionmentioning

confidence: 99%

Refolding of a Thermostable Glyceraldehyde Dehydrogenase for Application in Synthetic Cascade Biomanufacturing

Steffler

Sieber

2013

PLoS ONE

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The production of chemicals from renewable resources is gaining importance in the light of limited fossil resources. One promising alternative to widespread fermentation based methods used here is Synthetic Cascade Biomanufacturing, the application of minimized biocatalytic reaction cascades in cell free processes. One recent example is the development of the phosphorylation independent conversion of glucose to ethanol and isobutanol using only 6 and 8 enzymes, respectively. A key enzyme for this pathway is aldehyde dehydrogenase from Thermoplasma acidophilum, which catalyzes the highly substrate specific oxidation of d-glyceraldehyde to d-glycerate. In this work the enzyme was recombinantly expressed in Escherichia coli. Using matrix-assisted refolding of inclusion bodies the yield of enzyme production was enhanced 43-fold and thus for the first time the enzyme was provided in substantial amounts. Characterization of structural stability verified correct refolding of the protein. The stability of the enzyme was determined by guanidinium chloride as well as isobutanol induced denaturation to be ca. −8 kJ/mol both at 25°C and 40°C. The aldehyde dehydrogenase is active at high temperatures and in the presence of small amounts of organic solvents. In contrast to previous publications, the enzyme was found to accept NAD+ as cofactor making it suitable for application in the artificial glycolysis.

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Biosynthesis “debugged”: Novel bioproduction strategies

Cited by 53 publications

References 112 publications

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Cell-free Biosystems in the Production of Electricity and Bioenergy

Refolding of a Thermostable Glyceraldehyde Dehydrogenase for Application in Synthetic Cascade Biomanufacturing

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