Biosynthesis, Characterization of Some Combined Nanoparticles, and Its Biocide Potency against a Broad Spectrum of Pathogens

Abstract

The development of environmentally benign procedures for the synthesis of metallic nanoparticles (NPs) is a vital aspect in bionanotechnology applications for health care and the environment. This study describes the biosynthesis of Ag, Co, Ni, and Zn NPs by employing nanobiofactory Proteus mirabilis strain 10B. The physicochemical characterization UV-visible spectroscopy, scanning electron microscopy-energy-dispersive X-ray microanalysis (EDX), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS) technique including ζ potential, and polydispersity index (PDI) confirmed the formation of pure, stable monodisperse quasi-spherical oxide NPs of corresponding metals. The antimicrobial activity of biofabricated NPs was assessed against Gram-negative and Gram-positive bacteria, biofilm, yeast, mold, and algae via a well diffusion method. The results displayed significant antagonistic activity in comparison to their bulk and commercial antibiotics. Interestingly, the combined NPs exhibited promising synergistic biocide efficiency against examined pathogens which encourages their applications in adjuvant therapy and water/wastewater purification for controlling multiple drug-resistant microorganisms. To the best of our knowledge, no previous study reported the synthesis of semiconductor NPs by Proteus mirabilis and the biocide potency of combined NPs against a broad spectrum of pathogens not reported previously.

Sustainable microbial cell nanofactory for zinc oxide nanoparticles production by zinc-tolerant probiotic Lactobacillus plantarum strain TA4

“…This absorbance rate was due to the surface plasmon resonance (SPR) band of ZnO NPs. This finding was in an agreement with other studies which reported a similar absorption peak [53,54]. According to Eltarahony et al [54], it could be inferred that it was also due to the uniform particle size distribution.…”

“…This finding was in an agreement with other studies which reported a similar absorption peak [53,54]. According to Eltarahony et al [54], it could be inferred that it was also due to the uniform particle size distribution. Notably, it was also proven in this DLS analysis study that the biosynthesized ZnO NPs by TA4 cells were monodispersed in nature due to the ideal PDI value obtained at 0.369.…”

Extracellular fabrication of bio-nanostructures from Ralstonia sp. strain NS-7: Characterizations and their microbiological evaluation

“…spectroscopy. The AgNPs exhibited light yellow to brown color and it was observed due to excitation of surface plasmon vibration of the metal nanoparticles (25) . The characteristic UV-Vis absorption band maximum present at 420 nm confirmed the presence of spherical or roughly spherical AgNPs.…”

Detection of helminth ova genera using in-situ biosynthesis of gold nanoparticles

“…The conventional method for the synthesis of AuNPs involves the reduction of gold ions by reducing agents such as alkanethiols and alkylamines, or polymeric materials such as polyvinyl alcohol and gelatin to stabilise the AuNPs from aggregation [13]. However the use of capping agents for stabilisation and toxic chemicals for reductions may result in the formation of hazardous by-products, adversely affecting their use in diagnostics [14]. To overcome such limitations, various microbes [15] and biomacromolecules [16] have been used to synthesise AuNPs through redox reactions.…”