Biosynthesis, characterization of PLGA coated folate-mediated multiple drug loaded copper oxide (CuO) nanoparticles and it’s cytotoxicity on nasopharyngeal cancer cell lines

Abstract

Cytotoxicity of CuO nanoparticles (NPs) are an impediment in utilizing them as an effective nanocarriers of chemotherapeutic drugs for targeted drug delivery in nasopharyngeal cancer. In our current study, we have designed a two-step synthesis and coating of CuO NPs with different concentrations of PLGA (polylactide-co-glycolide) to reduce the cytotoxicity. This was further conjugated with folic acid to enhance targeting to specific tissue. The multiple drugs loaded in the NPs were two potent anticancer drugs doxorubicin and docetaxel. A complete characterization studies including micrographic analysis, zeta potential measurements, polydispersity index, Fourier transform infrared spectroscopy (FTIR), encapsulation and loading efficiencies, stability and in vitro release studies were done. Cytoxicity studies were done with MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, acridine orange/ethidium bromide and DAPI (4, 6-diamidino-2-phenylindole, dihydrochloride) staining procedures. Impediametric studies were also carried out to reinforce the reduction in cytotoxicity. Finally the cellular uptake of the NPs was seen. It was evident from the results that the multiple drugs loaded CuO NPs formed with PLGA coating were uniform, non-agglomerated in size ranging from 180 to 195 nm. The FTIR revealed no major changes in drug peaks. Encapsulation and loading efficiencies showed sufficient amount of drug being loaded into the NPs. The drug loaded NPs showed no change in size or zeta potential even after a period of 30 days. The cytotoxicity studies revealed significant reduction in toxicity after coating the surface treated with PLGA as evident from the microscopic analysis of cells. Hence the current study may be prioritized and further in vivo/in vitro studies may be carried out.

Induction of mitochondria mediated apoptosis in human ovarian cancer cells by folic acid coated tin oxide nanoparticles

“…This may be due to the presence of residual free carboxylic acid groups (−COO -) of FA. Similar findings were previously mentioned for shifting the negative values of the zeta potential toward the positive one for different nanoparticles after FA conjugation [15,[69][70][71].…”

One Pot Doxorubicin Partitioning and Encapsulation on Silica Nanoparticle, Applying Aqueous Two Phase System for Preparation of pH-Responsive Nanocarriers

“…The broad band around the 3200-3800 cm -1 region indicates the formation of hydrogen bonding between the top phase components 47,79,85,86 . The peak of the carbonyl group of DOX appears at 1730 cm -1 , while the peaks standing for the OH of primary amine and NH appear at 3330 and 3540 cm -1 , respectively [87][88][89][90] . Although DOX peaks were overlapped with the signals of PEG and lysine in DOX@nanoformulation spectrum, DOX molecules can be incorporated into the complexes formed by hydrogen bonding due to the presence of phenolic, amino and hydroxyl groups in its structure, which can form hydrogen bonding, and lead to the formation of a drug-loaded nanoformulation 69,70 .…”

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“…Although the antibacterial mechanism of copper has not been fully understood, recently, this ion has been widely used due to its usage in nanoparticle fabrication. Many studies have identified the anticancer properties of CuO nanoparticles [21]. CuO nanoparticles disrupt the cellular communication network by perturbation of cell signaling pathways.…”