Biosynthesis, characterization, and investigation of antimicrobial and cytotoxic activities of silver nanoparticles using Solanum tuberosum peel aqueous extract

Xu, Jiajun; Yıldıztekin, Mahmut; Han, Dayong; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Eftekhari, Aziz; Ava, Canan Aytuğ; Kandemir, Sevgi İrtegün; Cebe, Deniz Bariş; Dağ, Beşir; Beilerli, Aferin; Khalilov, Rovshan

doi:10.1016/j.heliyon.2023.e19061

Cited by 24 publications

(4 citation statements)

References 51 publications

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“…Cell viability was measured by MTT Cell Viability Assay kit and cytotoxicity was measured by LDH Cytotoxicity Assay Kit. All steps were performed in strict accordance with the instructions [ 53 ] (Xu et al, 2023b).…”

Section: Methodsmentioning

confidence: 99%

“…In a clinical study, the researchers pointed out that remote ischemic preconditioning did not improve the clinical outcomes of patients who received elective on-pump coronary artery bypass surgery (with or without valve surgery) [ 9 ] (Dj et al, 2015). New researches indicate that the new nano silver particles have good antibacterial and anti-cellular oxidation effects, but whether they have therapeutic effects on perioperative myocardial ischemia–reperfusion injury is still unknown [ 2 , 52 ]). Therefore, more effective treatments are imperative.…”

Section: Introductionmentioning

confidence: 99%

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Mild therapeutic hypothermia upregulates the O-GlcNAcylation level of COX10 to alleviate mitochondrial damage induced by myocardial ischemia–reperfusion injury

Deng,

Chen,

Zhang

et al. 2024

J Transl Med

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Objective Mild therapeutic hypothermia (MTH) is an important method for perioperative prevention and treatment of myocardial ischemia–reperfusion injury (MIRI). Modifying mitochondrial proteins after protein translation to regulate mitochondrial function is one of the mechanisms for improving myocardial ischemia–reperfusion injury. This study investigated the relationship between shallow hypothermia treatment improving myocardial ischemia–reperfusion injury and the O-GlcNAcylation level of COX10. Methods We used in vivo Langendorff model and in vitro hypoxia/reoxygenation (H/R) cell model to investigate the effects of MTH on myocardial ischemia–reperfusion injury. Histological changes, myocardial enzymes, oxidative stress, and mitochondrial structure/function were assessed. Mechanistic studies involved various molecular biology methods such as ELISA, immunoprecipitation (IP), WB, and immunofluorescence. Results Our research results indicate that MTH upregulates the O-GlcNACylation level of COX10, improves mitochondrial function, and inhibits the expression of ROS to improve myocardial ischemia–reperfusion injury. In vivo, MTH effectively alleviates ischemia–reperfusion induced cardiac dysfunction, myocardial injury, mitochondrial damage, and redox imbalance. In vitro, the OGT inhibitor ALX inhibits the OGT mediated O-GlcNA acylation signaling pathway, downregulates the O-Glc acylation level of COX10, promotes ROS release, and counteracts the protective effect of MTH. On the contrary, the OGA inhibitor ThG showed opposite effects to ALX, further confirming that MTH activated the OGT mediated O-GlcNAcylation signaling pathway to exert cardioprotective effects. Conclusions In summary, MTH activates OGT mediated O-glycosylation modified COX10 to regulate mitochondrial function and improve myocardial ischemia–reperfusion injury, which provides important theoretical basis for the clinical application of MTH.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mild therapeutic hypothermia upregulates the O-GlcNAcylation level of COX10 to alleviate mitochondrial damage induced by myocardial ischemia–reperfusion injury

Deng,

Chen,

Zhang

et al. 2024

J Transl Med

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show abstract

“…Both 3-dimensional and plane images were obtained to achieve a complete overview of the prepared nanoparticles. The measurements of the particle such as diameter and height were determined using NOVA 1.1.0.1921 software [ 30 ].…”

Section: Meterials and Methodsmentioning

confidence: 99%

“…Standard runs using a 30 kV voltage, a 20 mA current. The nanoformulations were analyzed at a 2θ angle which ranges from 5° to 40° at a scanning speed of 2° min–1 [ 30 ].…”

Section: Meterials and Methodsmentioning

confidence: 99%