Biosynthesis, characterization and enzymatic transesterification of single cell oil of Mucor circinelloides – A sustainable pathway for biofuel production

Carvalho, Ana Karine Furtado de; Rivaldi, Juan Daniel; Barbosa, Jayne C.S.; Castro, Heizir F. de

doi:10.1016/j.biortech.2014.12.110

Cited by 87 publications

(62 citation statements)

References 34 publications

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“…The lipid content achieved by CA is among the highest [10,11,13,44,45,62,63]. 3), being these values within the range referenced by other authors [46]. The lipid yield, glucose consumption, and lipid content showed that in CA approach, the consumed glucose was actively channelled for lipid biosynthesis.…”

Section: Combination Of High Aeration and 1% Of N-dodecane In Lipid Asupporting

confidence: 67%

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A new combined approach to improved lipid production using a strictly aerobic and oleaginous yeast

Guerreiro

Constantino

Lima-Costa

et al. 2018

Engineering in Life Sciences

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Microbial lipids have potential applications in energy, and food industry, because most of those lipids are triacylglycerol with long‐chain fatty‐acids that are comparable to conventional vegetable oils and can be obtained without arable land requirement. Rhodosporidium toruloides is a strictly aerobic strain, where oxygen plays a crucial role in growth, maintenance, and metabolite production, such as lipids and carotenoids. Dissolved oxygen concentration is one of the major factors affecting yeast physiological and biochemical characteristics. In this context, different approaches have been developed to increase available oxygen by the increasing the aeration and the addition of an oxygen‐vector. The growth of R. toruloides in 2‐L mechanical stirred tank reactor equipped with 1 or 2 porous spargers and a 70 C/N ratio, revealed a lipid content of 0.47 and 0.52 g/g and a lipidic productivity of 0.16 and 0.17 g/L day, respectively. The oxygen‐vector addition, increased the lipidic productivity for 0.20 g/L day and a lipid contend of 0.51 g of lipids/g of biomass. The combined approach, combining high aeration (AA), and 1% of n‐dodecane addition (DA), produced a significant improvement in the lipid accumulation (62%, w/w), when compared with the DA (51%, w/w) and the AA (52%, w/w) approaches. The increasing of lipids accumulation and smaller culture time are key factors for the success of scale‐up and profitability of a bioprocess.

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Section: Combination Of High Aeration and 1% Of N-dodecane In Lipid Asupporting

confidence: 67%

“…R. obliquus[64], A. pullulans[65] and M. circinelloides URM 4182[46]. obliquus[64], A. pullulans[65] and M. circinelloides URM 4182[46].…”

mentioning

confidence: 99%

A new combined approach to improved lipid production using a strictly aerobic and oleaginous yeast

Guerreiro

Constantino

Lima-Costa

et al. 2018

Engineering in Life Sciences

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“…The lipid content of M . circinelloides is typically around 25% [65], although gravimetric lipid yields up to 44% also have been reported [66]. The FTIR spectrum of the M .…”

Section: Resultsmentioning

confidence: 99%

FTIR Spectroscopy for Evaluation and Monitoring of Lipid Extraction Efficiency for Oleaginous Fungi

et al. 2017

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To assess whether Fourier Transform Infrared (FTIR) spectroscopy could be used to evaluate and monitor lipid extraction processes, the extraction methods of Folch, Bligh and Lewis were used. Biomass of the oleaginous fungi Mucor circinelloides and Mortierella alpina were employed as lipid-rich material for the lipid extraction. The presence of lipids was determined by recording infrared spectra of all components in the lipid extraction procedure, such as the biomass before and after extraction, the water and extract phases. Infrared spectra revealed the incomplete extraction after all three extraction methods applied to M.circinelloides and it was shown that mechanical disruption using bead beating and HCl treatment were necessary to complete the extraction in this species. FTIR spectroscopy was used to identify components, such as polyphosphates, that may have negatively affected the extraction process and resulted in differences in extraction efficiency between M.circinelloides and M.alpina. Residual lipids could not be detected in the infrared spectra of M.alpina biomass after extraction using the Folch and Lewis methods, indicating their complete lipid extraction in this species. Bligh extraction underestimated the fatty acid content of both M.circinelloides and M.alpina biomass and an increase in the initial solvent-to-sample ratio (from 3:1 to 20:1) was needed to achieve complete extraction and a lipid-free IR spectrum. In accordance with previous studies, the gravimetric lipid yield was shown to overestimate the potential of the SCO producers and FAME quantification in GC-FID was found to be the best-suited method for lipid quantification. We conclude that FTIR spectroscopy can serve as a tool for evaluating the lipid extraction efficiency, in addition to identifying components that may affect lipid extraction processes.

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“…On the other hand, the ability of Metschnikowia sp. to produce PUFAs at a concentration of > 10 % is an interesting feature that could be of use in lipid applications in food, and in pharmaceutical and cosmetic formulations (Carvalho et al 2015). On the other hand, the use of low-cost lipid production by Metschnikowia yeast in non-sterile conditions (Santamauro et al 2014), makes the cultivation of this oleaginous yeast in crude glycerol very attractive.…”

Section: Fatty Acid Compositionmentioning

confidence: 99%

Conversion of raw glycerol to microbial lipids by new Metschnikowia and Yarrowia lipolytica strains

et al. 2016

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Nine Metschnikowia spp. strains and six Yarrowia lipolytica strains were tested for lipid production on raw glycerol from a biodiesel plant. Metschnikowia sp. 271 and Y. lipolytica 347 were selected for biomass and lipid production, and optimized using central composite factorial design (CCD) and response surface methodology (RSM). The lipid production (2.60 g/L) of Y. lipolytica 347 was maximized at C/N 118, time 144 h and 90 g/L glycerol, while the maximum lipid production (0.49 g/L) of Metschnikowia sp. 271 was obtained under similar conditions but after a further incubation for 7 days at 16°C. The fatty acids profile of Y. lipolytica exhibited a considerable amount of C18:1n7 (~36 %), C18:1n9 (~16 %), and C16:0 (~16 %), whereas Metschnikowia sp. produced mainly C18:1n9 (~33 %), C16:0 (~21 %), and C16:1n7 (~21 %). Both yeasts showed similar amounts of unsaturated fatty acids (~70 %). However, considerable amounts of polyunsaturated fatty acid (PUFAs) were exhibited only by Metschnikowia sp. (~12 %).

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Biosynthesis, characterization and enzymatic transesterification of single cell oil of Mucor circinelloides – A sustainable pathway for biofuel production

Cited by 87 publications

References 34 publications

A new combined approach to improved lipid production using a strictly aerobic and oleaginous yeast

A new combined approach to improved lipid production using a strictly aerobic and oleaginous yeast

FTIR Spectroscopy for Evaluation and Monitoring of Lipid Extraction Efficiency for Oleaginous Fungi

Conversion of raw glycerol to microbial lipids by new Metschnikowia and Yarrowia lipolytica strains

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