Biosynthesis and structural characteristics of selenium nanoparticles by Pseudomonas alcaliphila

Zhang, Wenjie; Chen, Zhijuan; Líu, Hao; Zhang, Liang; Gao, Ping; Li, Daping

doi:10.1016/j.colsurfb.2011.06.031

Cited by 234 publications

(146 citation statements)

References 28 publications

(28 reference statements)

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“…Proteins with imino groups were easily bound to elemental Se through formation of Se-NH-bonds, which leads to highly stable SeNPs structure. 26 Similarly herein, rates of crystal growth and morphology of SeNPs are controlled by proteins in cancer cells.…”

Section: ■ Discussion Endogenous Senps Self-assembly In Cancer Cellsmentioning

confidence: 99%

Selenite-Induced Toxicity in Cancer Cells Is Mediated by Metabolic Generation of Endogenous Selenium Nanoparticles

et al. 2015

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Selenite has been a touted cancer chemopreventative agent but generates conflicting outcomes. Multiple mechanisms of selenite cytotoxicity in cancer cells are thought to be induced by metabolites of selenite. We observed that intracellular metabolism of selenite generates endogenous selenium nanoparticles (SeNPs) in cancer cells. Critical proteins that bind with high affinity to elemental selenium during SeNPs self-assembly were identified through proteomics analysis; these include glycolytic enzymes, insoluble tubulin, and heat shock proteins 90 (HSP90). Sequestration of glycolytic enzymes by SeNPs dramatically inhibits ATP generation, which leads to functional and structural disruption of mitochondria. Transcriptome sequencing showed tremendous down-regulation of mitochondrial respiratory NADH dehydrogenase (complex I), cytochrome c oxidase (complex IV), and ATP synthase (complex V) in response to glycolysis-dependent mitochondrial dysfunction. Sequestration of insoluble tubulin led to microtubule depolymerization, altering microtubule dynamics. HSP90 sequestration led to degradation of its downstream effectors via autophagy, ultimately resulting in a cell-signaling switch to apoptosis. Additionally, the surface effects of SeNPs generated oxidative stress, thus contributing to selenite cytotoxicity. Herein, we reveal that the multiple mechanisms of selenite-induced cytotoxicity are caused by endogenous protein-assisted self-assembly of SeNPs and suggest that endogenous SeNPs could potentially be the primary cause of selenite-induced cytotoxicity.

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Section: ■ Discussion Endogenous Senps Self-assembly In Cancer Cellsmentioning

confidence: 99%

Selenite-Induced Toxicity in Cancer Cells Is Mediated by Metabolic Generation of Endogenous Selenium Nanoparticles

et al. 2015

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“…E5 (Durán et al 2015), B. licheniformis (Khiralla and El-Deeb 2015), B. subtilis (Wang et al 2010), etc. Other reported aerobic strains are Pseudomonas agglomerans, (Torres et al 2012), P. aeruginosa JS-11 (Dwivedi et al 2013), P. stutzeri (Lortie et al 1992), P. alcaliphila (Zhang et al 2011), Duganella sp., Agrobacterium sp. (Bajaj et al 2012), Zooglea ramigera (Srivastava and Mukhopadhyay 2013), Streptomyces minutiscleroticus M10A62 (Ramya et al 2015), Acinetobacter sp.…”

Section: Selenite Reduction and Biogenesis Of Selenium Nanoparticlesmentioning

confidence: 99%

“…The C-O stretches of ether groups were noted at 1049 cm −1 . The involvement of proteins in the reduction and stabilization of selenium nanoparticles was reported in biosynthesis of selenium nanoparticles using bacteria Streptomyces minutiscleroticus M10A62 (Ramya et al 2015) and P. alcaliphila (Zhang et al 2011).…”

Section: Involvement Of Biomolecules In Synthesis and Stabilization Omentioning

confidence: 99%

“…HN-41 (Tam et al 2010), Bacillus sp. , Escherichia coli K-12 (Dobias et al 2011), P. alcaliphila (Zhang et al 2011), and Pantoea agglomerans (Torres et al 2012). It is well known that the selenium nanoparticles generated by biological reduction are, indeed, characteristically amorphous in nature (Torres et al 2012).…”

Section: Crystallographic Structure Of Selenium Nanoparticlesmentioning

confidence: 99%

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Bacillus cereus, selenite-reducing bacterium from contaminated lake of an industrial area: a renewable nanofactory for the synthesis of selenium nanoparticles

Kora

2018

Bioresour. Bioprocess.

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Background: An attempt was made to isolate selenite-reducing bacteria from a contaminated lake that receives industrial effluents and domestic sewage. The isolated dominant bacterial strain AJK3 was identified as Bacillus cereus, based on biochemical characterization and 16S rDNA sequencing. The time dependent selenium removal at different selenite concentrations monitored with ICP-AES indicates the substantial selenite reduction capability of the isolated strain. The selenium nanoparticles produced during the bacterial reduction of selenite were analyzed with UV-visible spectroscopy, X-ray diffraction, transmission electron microscopy, zeta potential measurement, Fourier transform infrared spectroscopy and Raman spectroscopy. Results:The nanoparticle synthesis was confirmed from the red colour emergence in culture broth and wide UV-vis peaks. The produced nanoparticles were polydisperse, spherical, size varied from 50 to 150 nm and the mean particle size was about 93 nm. The amorphous nature of the generated nanoparticles was confirmed from the Raman spectroscopy, XRD and SAED patterns. The IR data and zeta potential values substantiated the protein capping of the produced nanoparticles. Conclusions:Thus, the present study suggests that the isolated bacterial strain can be exploited as a prospective, renewable, natural, nanofactory for the bacteriogenic synthesis of nanoparticles. Also, the study has application in bioremediation of selenite from the contaminated environment.

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“…dissolution, entry into cells, surface chemistry, and reactivity) into consideration (Buchs et al, 2013;Winkel et al, 2012). Although the average particle size of nano-Se b is more than 100 nm, microbially produced Se(0) is often referred to as nano-Se in the literature (Jain et al, 2015;Srivastava & Mukhopadhyay, 2013;Zhang et al, 2011). In addition, the particles of nano-Se b formed by microorganisms are colloidal in nature (Buchs et al, 2013); hence the term nano-selenium is used in this paper.…”

Section: Introductionmentioning

confidence: 99%

A comparison of fate and toxicity of selenite, biogenically, and chemically synthesized selenium nanoparticles to zebrafish (Danio rerio) embryogenesis

et al. 2017

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Biosynthesis and structural characteristics of selenium nanoparticles by Pseudomonas alcaliphila

Cited by 234 publications

References 28 publications

Selenite-Induced Toxicity in Cancer Cells Is Mediated by Metabolic Generation of Endogenous Selenium Nanoparticles

Selenite-Induced Toxicity in Cancer Cells Is Mediated by Metabolic Generation of Endogenous Selenium Nanoparticles

Bacillus cereus, selenite-reducing bacterium from contaminated lake of an industrial area: a renewable nanofactory for the synthesis of selenium nanoparticles

A comparison of fate and toxicity of selenite, biogenically, and chemically synthesized selenium nanoparticles to zebrafish (Danio rerio) embryogenesis

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